Apparatus and method for fast quantitative bacteria detection

Abstract

The invention provides a device and a method for rapid quantitative detection of bacteria. The method comprises the following steps: the immune technique is adopted for marking bacteria in a sample with a fluorescent substance (such as FITC, fluorescence quantum dots, and the like); as a sample solution flows through a set of filtering device, the bacteria marked with the fluorescent substance settle on the surface of a filtering membrane; the fluorescent substance on the bacteria emits fluorescence by adopting laser to induce and arouse fluorescence; and the number of the bacteria in the sample can be obtained quickly and accurately by detecting the signal strength of all the fluorescence on the filtering membrane. The entire detecting process lasts for less than 30 minutes.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a device and method for rapid quantitative detection of bacteria. Background technique [0002] Bacteria, especially pathogenic bacteria, pollute human drinking water and food, and bring potential danger to human health. For example, enterohemorrhagic Escherichia coli can cause hemorrhagic enteritis and even death in humans. In 1999, a large-scale outbreak also occurred in my country. [0003] The routine detection methods for the total number of bacteria mainly include the national standard method of counting bacterial colonies by using the plate count method after culturing the sample in a petri dish for 24-48 hours. These tests require a number of complicated analysis tests and preparations in the laboratory The culture medium requires a long time due to the need for cultivation, and special equipment is needed for counting, and due to the lack of specificity, it is imposs...

AI Technical Summary

Problems solved by technology

[0003] The routine detection methods for the total number of bacteria mainly include the national standard method of counting bacterial colonies by using the plate count method after culturing the sample in a petri dish for 24-48 hours. These tests require a number of complicated analysis tests and preparations in the laboratory Culture medium, due to the need for cultivation, takes a long time, and requires special equipment to count, and because it is not specific, it cannot distinguish pathogenic bacteria with similar shapes

The above process often requires professional and technical personnel to complete, and it is difficult to achieve on-site rapid detection

At present, some more rapid and sensitive bacterial detection methods are being researched and developed at home and abroad. There are already complete sets of kits or test strips on the market, mainly to estimate the number of bacteria in the sample by comparing the color changes of the test strips. Although this method The detection speed is fast, but the sensitivity is low, and it is difficult to achieve quantitative detection

The ATP method is also used on the market to quickly detect the total number of bacteria in the sample. This is a bioluminescence method for the detection of the total number of bacteria, but this method is not specific and cannot distinguish the bacterial species in the sample.

In addition, nucleic acid amplification technology and microfluidic chip technology are currently extensively researched detection methods, but these technologies are still in the laboratory research stage and cannot meet the actual needs of rapid on-site detection of bacteria

Images