Ractopamine immunogen, coating antigen and use thereof in colloid-gold test paper

A technology of ractopamine and immunogen, which is applied in the field of immunology, can solve the problem that the detection limit cannot meet the national limit requirements, and achieve the effect of improving immunogenicity
CN101993488AActive Publication Date: 2011-03-30深圳市三方圆生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
深圳市三方圆生物科技股份有限公司
Publication Date
2011-03-30

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Abstract

The invention relates to a ractopamine immunogen, a ractopamine coating antigen and use thereof in colloid-gold test paper and belongs to the technical field of immunology. The immunogen and coating antigen are products of the coupling of the hapten of the connecting arm of a hydroxyl terminal far away from a nitrogen atom of the ractopamine with a carrier protein. The hapten is similar to a ractopamine counterpart in terms of molecular structure, stereochemistry and electronic distribution. The molecule of the hapten has an active group for coupling with a protein carrier, and the existence of the active group exerts on influences on the electronic distribution of the molecules of a material to be tested. When coupled with hemocyanin, the hapten enables an organism to produce a high-potency and high-specificity antibody against ractopamine and be absorbed onto a glass cellulose membrane; and the coating antigen constructed by coupling with human haemocyanin can be absorbed onto a nitrocellulose membrane for constructing the colloid-gold test paper.
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Description

technical field

[0001] The invention relates to a ractopamine immunogen and a coater, belonging to the technical field of immunology. Background technique

[0002] Ractopamine hydrochloride is a pharmaceutical raw material, a cardiotonic drug that can be used to treat congestive heart failure. It can also be used to treat muscular dystrophy, increase muscle and reduce fat accumulation. At the same time, it can increase the daily weight gain of animals, improve the utilization rate of feed, and increase the protein content of animals. Ractopamine is a beta stimulant, which can improve the growth rate and efficiency of lean tissue when added to pig feed, and the skin is smooth and smooth, and looks good. The poisoning phenomenon of ractopamine is similar to the poisoning phenomenon of "clenbuterol" when people consume ractopamine. Symptoms such as rapid heartbeat, tremor, and palpitations may occur, and more serious ones may cause human teratogenicity and carcinogenesis. Th...

Claims

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