Disease resistance evaluation method of cultured shrimps and crabs
A technology for traits and shrimp and crabs, applied in the field of evaluation of disease resistance traits of cultured shrimp and crabs, can solve problems such as unclear evaluation indicators of disease resistance traits of cultured shrimp and crabs, achieve accurate evaluation results, and overcome limitations and uncertainties
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[0022] A method for evaluating the disease resistance traits of cultured shrimp and crabs. Different cultured shrimps and crabs are infected with the same amount of pathogens according to the muscle weight ratio, and a control group is set up. One hour after infection, the hepatopancreas of each species are collected for the determination of the pathogen content in the body, so as to obtain Pathogen removal ability of infected subjects; 24 hours after infection, hemolymph and hepatopancreas were collected for the determination of blood cell count, hemocyanin content, plasma AKP activity, and 5 immune gene indexes of ALF, crustin, lysozyme, cathepsin B, and LGBP. After standardizing the values of 9 indicators including clearance, blood cell count, hemocyanin content, plasma AKP activity, ALF, crustin, lysozyme, cathepsin B and LGBP, the disease resistance index f of cultured shrimp and crabs was calculated by principal component analysis. The larger the f value, the stronger t...
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