Disease resistance evaluation method of cultured shrimps and crabs

A technology for traits and shrimp and crabs, applied in the field of evaluation of disease resistance traits of cultured shrimp and crabs, can solve problems such as unclear evaluation indicators of disease resistance traits of cultured shrimp and crabs, achieve accurate evaluation results, and overcome limitations and uncertainties

Active Publication Date: 2016-10-26
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for evaluating the disease resistance traits of cultured shrimp and crabs, so as to solve the problem that the evaluation indicators of the disease resistance traits of cultured shrimp and crabs in my country are not clear.

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  • Disease resistance evaluation method of cultured shrimps and crabs
  • Disease resistance evaluation method of cultured shrimps and crabs
  • Disease resistance evaluation method of cultured shrimps and crabs

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Embodiment 1

[0022] A method for evaluating the disease resistance traits of cultured shrimp and crabs. Different cultured shrimps and crabs are infected with the same amount of pathogens according to the muscle weight ratio, and a control group is set up. One hour after infection, the hepatopancreas of each species are collected for the determination of the pathogen content in the body, so as to obtain Pathogen removal ability of infected subjects; 24 hours after infection, hemolymph and hepatopancreas were collected for the determination of blood cell count, hemocyanin content, plasma AKP activity, and 5 immune gene indexes of ALF, crustin, lysozyme, cathepsin B, and LGBP. After standardizing the values ​​of 9 indicators including clearance, blood cell count, hemocyanin content, plasma AKP activity, ALF, crustin, lysozyme, cathepsin B and LGBP, the disease resistance index f of cultured shrimp and crabs was calculated by principal component analysis. The larger the f value, the stronger t...

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Abstract

The invention discloses a disease resistance evaluation method of cultured shrimps and crabs, and belongs to the technical field of aquatic products. Infection with equivalent pathogens is performed according to muscle weight proportions of the cultured shrimps and crabs, nine indexes of the cultured shrimps and crabs are measured including pathogen clearance power, blood cell quantities, haemocyanin contents, blood plasma AKP vitality, ALF, crustin, lysozyme, cathepsin B and LGBP, a disease resistance exponent f of an infected object is obtained by use of principal component analysis, it is indicated that the bigger the f value is, the stronger the disease resistance is, and the smaller the f value is, the weaker disease resistance is. A set of exponents adapted to integrate evaluation of the disease resistance of the cultured shrimps and crabs is constructed for solving the problem of lack of similar technologies in the field, the evaluation result is more accurate, and restrictions and uncertainties of the mode of evaluating the disease resistance through a single immunization index are overcome.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for evaluating disease resistance traits of cultured shrimp and crabs. Background technique [0002] The annual output of shrimp and crab farming is nearly 4 million tons, which occupies an important position in my country's aquaculture. The cultured shrimps mainly include Litopenaeus vannamei, Penaeus monodon, Penaeus chinensis, Penaeus japonicus in seawater, green shrimp, Macrobrachium rosenbergii and Procambarus clarkii in freshwater. The cultured crabs mainly include Portunus trituberculatus in seawater, blue crabs in burrows and Chinese mitten crabs in freshwater. [0003] Bacteria and viruses are the two most harmful infectious pathogens in shrimp and crab farming, which can cause outbreaks of shrimp and crab diseases and cause huge economic losses. Among them, Vibrio among bacteria is the most common pathogenic bacteria, most of which are conditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李健葛倩倩刘萍李吉涛
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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