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Preparations and uses of streptomycin-carrier protein coupled product and streptomycin antibody

A carrier protein, streptomycin technology, applied in chemical instruments and methods, ovalbumin, albumin peptides, etc., can solve the problems of complicated operation, expensive instruments, high cost, and achieve the effects of simple and convenient operation, simple and effective methods

Inactive Publication Date: 2009-04-15
ZHEJIANG UNIV
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Problems solved by technology

[0003] At present, the detection of streptomycin residues mainly includes instrumental analysis and immunoassay. Among them, instrumental analysis mainly uses high-performance liquid chromatography (HPLC) and gas chromatography (GC). , high cost, incapable of large-scale on-site testing, etc., also requires technicians to have certain operation and analysis skills, so it cannot be well popularized and applied

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  • Preparations and uses of streptomycin-carrier protein coupled product and streptomycin antibody
  • Preparations and uses of streptomycin-carrier protein coupled product and streptomycin antibody

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Embodiment Construction

[0024] The invention provides an immunological method for synthesis of streptomycin artificial antigen, preparation of antibody and detection of its residue in food. Use the prepared immunogen to prepare streptomycin with strong specificity, high sensitivity, good stability, and mass-produced monoclonal antibodies, and use these monoclonal antibodies to establish a highly specific, sensitive and highly specific method for detecting streptomycin residues in food. The rapid diagnostic method of correctness—competitive ELISA and immunochromatographic test strips can be effectively used in the detection of streptomycin residues in animal products and serve the food safety of our country.

[0025] 1. a preparation method of streptomycin and carrier protein coupling product comprises the steps:

[0026] a) Dissolve 50-1600mg streptomycin and 30-300mg carboxymethylhydroxylamine in 2-5ml Milli-Q ultrapure water or double distilled water containing 5-20mg sodium acetate, stir for 15-30...

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Abstract

The invention discloses a coupling product of streptomycin and carrier protein, and a preparation method of a streptomycin antibody and an application thereof, which relate to a coupling method of the carrier protein such as keyhole limpet hemocyanin, human serum albumin, cow serum albumin, ovalbumin and the like with streptomycin, and immune BALB / C mouse spleen cells are fused with SP2 / 0 mouse myeloma cells by streptomycin immunogens coupled with the carrier protein, and the streptomycin coupled with the carrier protein is used as a coating antigen for screening positive hybridoma and hybridoma that can steady passage and excrete anti-specificity streptomycin monoclonal antibody can be obtained by the cell clone, and ascites monoclonal antibody is prepared. The prepared monoclonal antibody is used for building a direct competition ELISA method with high specificity, sensitivity and accuracy to the streptomycin and immunity colloidal gold test strips. The coupling of the streptomycin and the carrier protein and the preparation method of the streptomycin monoclonal antibody can provide services for detecting streptomycin residue in foods quickly.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and biology, and in particular relates to a preparation method and application of a streptomycin-carrier protein coupling product and a streptomycin antibody. Background technique [0002] Streptomycin is an aminoglycoside antibiotic, which was successfully developed by the Waxman Institute in the United States in 1945. It is widely used in the treatment of human and animal diseases: human streptomycin is used for tuberculosis, plague and Gram-negative The treatment of diseases caused by bacteria; Streptomycin on animals is effective for various diseases of chickens, sheep, cattle, and pigs, such as chicken infectious rhinitis, pig acute bronchitis, swine pullorum, dairy cow endometritis, colibacillosis, etc. Very good curative effect, so it is often used as a feed additive. But streptomycin has many side effects: 1) allergic reaction; 2) damage to the eighth cranial nerve; 3) inhibition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K14/77C07K14/765C07K16/00C07K16/44G01N33/577
Inventor 吴建祥张少恩陈正贤
Owner ZHEJIANG UNIV
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