D-dimer detection kit and preparation method thereof

Abstract

The invention relates to the technical field of biology, and discloses a D-dimer detection kit and a preparation method thereof. The kit comprises a buffer solution, D-dimer, a coagulation accelerator, a stabilizing agent, and an antiseptic; wherein the coagulation accelerator is polyvinylpyrrolidone (PVP). By adding polyvinylpyrrolidone (PVP) taken as a coagulation accelerator into the D-dimer detection kit, the combination between an antigen and an antibody is promoted to form a compound rapidly, at the same time, the dissociation of immune complex is inhibited, the precipitate appears rapidly, the detection speed is accelerated; moreover, the non-specific range is not enlarged, and the wide linear range of the D-dimer detection kit is maintained. Furthermore, PVP can fix D-dimer monoclonal antibodies on latex particles, the change of turbidity is specifically enhanced, and thus the detection sensitivity of the kit is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a D-dimer detection kit and a preparation method thereof. Background technique [0002] The fibrinolytic system is the most important anticoagulant system in the human body. It plays an important role in maintaining the normal permeability of the blood vessel wall, maintaining blood flow and tissue repair. The system is mainly composed of 4 parts: plasminogen, Plasminogen activator, plasmin, plasmin inhibitor. When a fibrin clot forms, in the presence of plasminogen activator, plasminogen is activated and converted into plasmin, fibrin begins to dissolve, and plasmin degrades fibrinogen and cross-links fibrin to form various Soluble fragments, collectively referred to as fibrinogen degradation products (FDP). Among them, D-dimer is the smallest fragment in the degradation products, and it is a specific degradation product of cross-linked fibrin. [0003] Under normal physiological...

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Problems solved by technology

Among them, the latex agglutination method is simple and fast, and is suitable for emergency detection, but it can only be used for qualitative or semi-quantitative detection, and is often used as a screening; the enzyme-linked immunosorbent assay method is accurate, quantitative, and highly sensitive, but the operation requirements are strict. , the steps are cumbersome, and are not suitable for rapid diagnosis and monitoring of emergency and clinical patients; the immunogold standard method has the characteristics of easy operation and rapidity of the latex agglutination method, and has the characteristics of accuracy and quantification of the ELISA method, but rheumatoid factor, heparin and blood lipids, etc. It interferes with the detection results to a certain extent, and is not suitable for large-scale promotion and use; latex immunoturbidimetric method has the advantages of simple operation, rapidity, accurate quantification, strong specificity, and high sensitivity, which can meet the needs of outpatient and emergency departments. more and more widely used

However, most of the existing D-dimer assay kits prepared by latex immunoturbidimetric method use polyethylene glycol or dextran as a coagulant, which also leads to the following disadvantages, for example, when the sensitivity of the kit is improved , it will cause shortcomings such as narrow linear range and poor stability; while the imported kit is expensive and the detection cost is high, which is not conducive to the promotion and application of this detection item in clinical diagnosis

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