Magnetic solid phase extraction material of aflatoxin B1 and B2, preparation method and application thereof

A kind of aflatoxin extraction technology, applied in the direction of material separation, analysis materials, instruments, etc., can solve the problems of high price, long cycle, high detection cost, etc.

Active Publication Date: 2019-05-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high requirements for sample purity, some pretreatment processes are required, resulting in high detection costs and long cycles, which cannot meet the requirements of rapid screening of large batches of samples.
Traditional pretreatment technologies include immunoaffinity columns, multifunctional purification columns, etc. These purification columns are more expensive and mostly disposable.

Method used

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  • Magnetic solid phase extraction material of aflatoxin B1 and B2, preparation method and application thereof
  • Magnetic solid phase extraction material of aflatoxin B1 and B2, preparation method and application thereof
  • Magnetic solid phase extraction material of aflatoxin B1 and B2, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 Aflatoxin B 1 and B 2Design and synthesis of nucleic acid aptamers

[0059] Design and synthesis of aflatoxin B with high specificity and high affinity 1 and B 2 Nucleic acid aptamer sequence, its nucleic acid sequence is shown in SEQ ID NO:1.

Embodiment 2

[0060] Embodiment 2 utilizes the aflatoxin B of amino modification 1 and B 2 Preparation of Magnetic Solid Phase Extraction Materials by Aptamers

[0061] 1. Fe 3 o 4 @SiO 2 Preparation of Magnetic Nanoparticles

[0062] 1g FeCl 3 ·6H 2 O and 6g of sodium acetate were dispersed into 180mL of ethylene glycol solution, magnetically stirred until homogeneous, added to a polytetrafluoroethylene autoclave, heated at 200°C for 10h, cooled to room temperature, and the product was collected by a magnet, washed repeatedly with water and ethanol for 3 times, dried in a vacuum oven at 60°C to obtain Fe 3 o 4 powder.

[0063] 1g of the above dried Fe 3 o 4 Disperse in 200mL ethanol and 100-500mL deionized water, ultrasonically disperse for 10min, add 1mL 25% ammonia solution, stir mechanically for 10min, mix 4mL tetraethyl orthosilicate and 20mL absolute ethanol and add dropwise to the above solution, One drop per second, after the dropwise addition, react overnight at room te...

Embodiment 3

[0076] Embodiment 3 utilizes magnetic solid phase extraction material to purify the aflatoxin B in the corn sample 1 and B 2 and its detection

[0077] In this embodiment, aflatoxin B is quantitatively added to normal corn samples 1 and B 2 The standard product is then purified by the magnetic solid phase extraction material prepared in Example 2, and then detected by high performance liquid chromatography-online photochemical derivatization-fluorescence detector after purification to determine the recovery rate. details as follows:

[0078] 1. Corn sample processing

[0079] 1) Pulverize the corn sample.

[0080] 2) Add aflatoxin B to the crushed corn samples respectively 1 and B 2 The standard and spiked water products are 0.5μg / Kg, 5μg / Kg, and 50μg / Kg respectively.

[0081] 3) Weigh 5 g of the sample, add 25 mL of methanol-water (70:30, v / v), and place it on a homogenizer for 11,000 rpm high-speed homogenization for 3 min.

[0082] 4) Take 5 mL of the filtrate, blo...

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Abstract

The invention provides a magnetic solid phase extraction material of aflatoxin B1 and B2, a preparation method and application thereof. The magnetic solid phase extraction material, by adopting superparamagnetic Fe3O4@SiO2 as a core and natural hydrophilic agarose as a matrix, encapsulates agarose on the surface of Fe3O4@SiO2 by a composite emulsification technique, and then modifies the NHS groupon the surface of the agarose, thereby being capable of binding to the aptamer by covalent coupling. Compared with traditional carboxyl and amino magnetic beads, magnetic agarose microspheres containing NHS groups on the surface do not need to be activated with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or glutaraldehyde, and the aptamer can be covalently coupled to the magnetic beads by mixing the aptamer solution with NHS magnetic agarose magnetic beads for 1 to 2 hours at room temperature. The magnetic solid phase extraction material provided by the invention is used as a novel magneticsolid phase extraction adsorbent for the detection and analysis of aflatoxins B1 and B2 in food, agricultural products and traditional Chinese medicine.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular, relates to aflatoxin B 1 and B 2 Magnetic solid phase extraction materials, preparation methods and applications. Background technique [0002] Aflatoxin B (Aflatoxin B, AFB) is a kind of structurally similar toxic secondary metabolites produced by fungal species such as Aspergillus flavus and Aspergillus parasiticus, and the common ones are aflatoxin B 1 and aflatoxin B 2 , has strong carcinogenic, teratogenic and mutagenic effects. Among them, aflatoxin B 1 The toxicity of potassium cyanide is 10 times that of potassium cyanide and 68 times that of arsenic. It is classified as a class I carcinogen by the International Agency for Research on Cancer (IARC). Aflatoxin B is the most stable mycotoxin found so far. It is not easy to be destroyed under general food processing conditions, so it poses a huge hidden danger to consumers' dietary safety. About 25% of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘洪美栾云霞陆安祥付海龙郭晓军王纪华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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