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一种甘露二糖、组合物的技术，应用在肠内附着的饲料领域，能够解决抑制效果小等问题
Inactive Publication Date: 2006-01-04
JAPAN NUTRITION
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In addition, through the yeast agglutination test, it was found that manno-oligosaccharides and mannose-based polysaccharides with a molecular weight larger than mannobiose have little inhibitory effect on the adhesion of Salmonella
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Embodiment 1
[0022] Example 1 (Manufacture of β-1,4-mannobiose-containing composition from palm kernel flour)
[0023] 100 parts of palm kernel powder (Fuji Oil Co., Ltd.) with a mannan content of 36% and a water content of 8.2% was dissolved in an enzyme solution in which 0.25 parts of hemicellulase GM "Amano" (Amano Pharmaceutical Co., Ltd.) 150 parts were applied at 60° C. for 12 hours, and then dried to a moisture content of 9.8% with a fluidized bed drying apparatus (manufactured by Okawara Co., Ltd.) to obtain 102 parts of dry powder. As a result of measuring the content of mannose and β-1,4-mannobiose in this dry powder by ion exchange chromatography, 1.44 parts of mannose and 10.58 parts of β-1,4-mannose were produced. Disaccharides (29.4% relative to mannan; 11.5% in terms of dry weight).
Embodiment 2
[0024] Example 2 (Manufacture of β-1,4-mannobiose-containing composition from coconut flour)
[0025] 100 parts of coconut powder (mannan content 30%, moisture 4.2%), with 150 parts of enzyme solution dissolved with 0.25 parts of hemicellulase GM "Amano" (Amano Pharmaceutical Co., Ltd.), acted at 60 ° C for 12 hours Thereafter, the water content was dried to 9.3% with a fluidized bed drying apparatus to obtain 106 parts of dry powder. As a result of measuring the content of mannose and β-1,4-mannobiose in this dry powder, 1.36 parts of mannose and 12.35 parts of β-1,4-mannobiose (relative to mannose Glycans 41.2%; dry weight equivalent 12.9%).
Embodiment 3
[0028] Example 3 (Salmonella Yeast Agglutination Inhibition Test)
[0029] The agglutination inhibition test of Salmonella with various sugars was performed in the following manner. The number of bacteria containing Salmonella enteritidis (Salmonella enteritidis KTE-61 strain isolated by Itochu Feed Co., Ltd. 1×10 8 cfu / ml) suspension 0.1ml and distilled water 0.1ml were well mixed, and yeast Candiida albicans (Candida albicans KI-102001 purchased from the Association for Human and Animal Biological Preparations) suspension 0.1ml was added thereto and mixed . A preliminary experiment was carried out, that is, the agglutination reaction taking place in this mixture was confirmed with a stereomicroscope.
[0030] After the dried powder 0.5g obtained in Example 1 is mixed with 5ml water into a suspension, centrifuge at 10000rpm for 5 minutes, and its centrifugal supernatant is analyzed by high-speed liquid chromatography (manufactured by JASCO). 10 mg of beta-1,4-mannobiose. ...
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Abstract
The present invention is to provide a beta -1,4-mannobiose-containing composition which can inhibit colonization of salmonella in animal body and effectively excrete salmonella outside the body and the method for producing thereof, a feed additive containing the beta -1,4-mannobiose-containing composition, and a feed blended with the beta -1,4-mannobiose-containing composition. The present invention is intended to provide a method for producing the beta -1,4-mannobiose-containing composition wherein a mannan degrading enzyme is functioned to a mannan-containing natural material for producing at least 10% by weight of beta -1,4-mannobiose based on the mannan before the degradation, the beta -1,4-mannobiose-containing composition prepared through the function of a mannan degrading enzyme to a mannan-containing natural material, which contains at least 10% by weight of beta -1,4-mannobiose in terms of dry matter, a feed additive containing the beta -1,4-mannobiose-containing composition, and a feed blended with the beta -1,4-mannobiose-containing composition.
Description
technical field [0001] The present invention relates to a composition containing β-1,4-mannobiose (β-1,4-mannobiose), a method for producing the same, a feed additive containing the β-1,4-mannobiose composition, and a composition containing A feed containing a composition of β-1,4-mannobiose is particularly a feed capable of inhibiting the attachment (fixation) of Salmonella to the intestines of livestock or poultry. Background technique [0002] It has been known that palm kernel powder, coconut powder, guar powder, etc., contain a large amount of mannan containing mannose as a constituent sugar, and that mannose can be produced when enzymes act on these natural materials Sugars, mannan-oligosaccharides and mannan polysaccharides, many such methods have been developed. In addition, it is also known that the addition of these mannose substances to feed can inhibit the adhesion of harmful Salmonella in the intestines and have the effect of expelling them from the body (the e...
Claims
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