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Inspection method and verification for salmonella in lip care cosmetics

An inspection method, Salmonella technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., to achieve the effects of rapid quantification, improvement of product quality, and obvious selectivity effect

Inactive Publication Date: 2012-07-04
SHAANXI INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the detection method of Salmonella in lip care cosmetics in domestic and foreign literature reports and patent records

Method used

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  • Inspection method and verification for salmonella in lip care cosmetics

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Embodiment Construction

[0037] A kind of inspection method and verification for salmonella in lip care cosmetic products, the steps are:

[0038] 1 Samples and instruments, materials

[0039] 1.1 Sample Clinique Fruity Lip Gloss 02 (made in the United States, batch number: 6M2302)

[0040] 1.2 Instruments WGP-600 water-proof incubator (produced by Chongqing Wanda); CX-31 microscope (OLYMPUS, Japan)

[0041] 1.3 Materials

[0042] Liquid paraffin-produced by Li'an Longbohua Pharmaceutical Chemical Co., Ltd. Batch number: 201002

[0043] Polysorbate-80-Manufactured by Tianjin Kemiou Chemical Reagent Co., Ltd. Batch number: 20110617

[0044] Trypticase Soy Broth Medium-Beijing Sanyao Technology Development Co., Ltd. Batch number: 080121

[0045] Sodium Tetrathiosulfate Brilliant Green Growth Medium (TTB)-Beijing Land Bridge Technology Co., Ltd. Batch number: 110603

[0046] Magnesium Chloride-Malachite Green Salmonella Enrichment Medium (RVS)-Beijing Niuniu Gene Technology Co., Ltd. Batch number: 2...

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Abstract

The invention relates to an inspection method and verification for salmonella in lip care cosmetics. The inspection method comprises the following steps of: 1, performing resuscitation and hyperplasia culture on a sample; 2, separating and culturing by using a chromogenic medium; 3, performing an initial identification test on a culture; 4, performing microscopic examination; 5, performing a biochemical test; and 6, performing a serum agglutination test, wherein 5 and 6 are typical salmonella confirmation tests. By combining a method for detecting the salmonella in the national food safety standard, the requirement for detecting the salmonella in the lip care cosmetics is met, and due to an obvious selectivity effect, the interference of other bacteria in the sample is avoided, and the salmonella is quickly detected. The method provides theoretical guidance for inspecting the salmonella in the lip care cosmetics, and has important instructive significance for improving the quality of the lip care cosmetics.

Description

technical field [0001] The invention relates to an inspection method and verification for salmonella in lip care cosmetic products, belonging to the technical field of detection of pathogenic bacteria in food and cosmetics in biotechnology. Background technique [0002] Salmonella is a Gram-negative facultative anaerobic bacterium, which is an important pathogen of zoonotic intestinal infectious diseases. Its transmission route is mainly fecal-oral transmission, most of them are transmitted by food, and a few are transmitted by water. The largest Salmonella food poisoning in the world was in Sweden in 1953, due to Salmonella typhimurium poisoning caused by eating pork, 7717 people were poisoned and 90 people died. Due to the different types and strains of Salmonella, the amount of bacteria that cause human disease is also different. Generally, 10 6 ~10 7 cfu / g Salmonella food can cause disease. According to reports, salmonellosis is the second most frequent zoonotic infec...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/42
Inventor 张丹刘海静王嫦鹤李涛杨静
Owner SHAANXI INST FOR FOOD & DRUG CONTROL
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