51results about How to "Meet on-site inspection" patented technology

The invention provides a primer combination for detecting succulent plant stem fusarium moniliforme based on LAMP (Loop-mediated Isothermal Amplification) and application thereof. The LAMP primer combination for detecting the succulent plant stem fusarium moniliforme consists of 1 pair of outside primers F3/B3 and a pair of inside primer FIP/BIP, wherein the F3/B3 and FIP/BIP primer sequences areshown as SEQ ID NO. 1 to 4. By adopting the detection system disclosed by the invention, the succulent plant stem fusarium moniliforme can be detected rapidly, conveniently and efficiently at high specificity and high sensitively through incubation at the constant temperature of 65 DEG C for 1 hour without complex and expensive instruments, and in-situ detection of the succulent plant stem fusarium moniliforme can be met well. The detection system can be widely applied to early diagnosis of field diseases of establishment units as well as monitoring and identification of viruses, and a reliable technological and theoretical basis is provided for prevention and control of the succulent plant stem fusarium moniliforme.

The invention discloses a nucleic acid (DNA/RNA) real-time constant-temperature gene amplification detection method. The method is a rapid and efficient novel isothermal amplification detection method taking microplate reader detection as a core. The method particularly comprises the following steps that a to-be-detected sample DNA/RNR template is added into a 15 microliters-25 microliters of LAMP amplification system with certain neutral red concentration and then put into a microplate reader with the temperature ranging from 60 DEG C to 65 DEG C to detect change of absorbance of a reaction system in real time, and therefore the reaction is indirectly monitored. A reaction signal is expressed in the form of a curve, the curve enters a logarithmic phase within 30 min-60 min to express that a detected sample is positive, and the curve extends all the time but not enters the logarithmic phase to express that the detected sample is negative. The nucleic acid (DNA/RNA) real-time constant-temperature gene amplification detection method is simple, rapid and convenient, a great push function is achieved for subsequent basic-level popularization, compared with a traditional PCR detection method, rapid and safe detection of a gene is achieved, and an important significance in the aspects of blocking transmission routes, effectively controlling and tracking infection sources and effectively controlling outbreak and prevalence of superbacteria is achieved.

The invention discloses an LAMP detection primer composition for phytophthotacactorum, as well as an LAMP detection kit and an LAMP detection method of the LAMP detection primer composition. The LAMP detection primer composition consists of a positive inner primer FIP, a reverse inner primer BIP, a positive outer primer F3, a reverse outer primer B3 and a reverse loop primer LB; detailed sequences of all the primers are as follows: FIP: 5'-TCTGGGCACAACCGCAAAAA-TTTGCGAGCTCCAGATTTCC-3'; BIP: 5'-AATCCGTACGATCGAGCTGGAC-ACACGCCACGTCTGCT-3'; F3: 5'-TTCTGCGCTAGGCGACC-3'; B3: 5'-CACACAAGTGGACCGTTAG3'; LB: 5'-GGAAAGACCATCAAGCTCCAGAT-3'. The detection method disclosed by the invention is high in accuracy, high in specificity, convenient to operate and good in practicability; constant-temperature amplification is realized; meanwhile, a novel technology platform is provided for detection of the phytophthotacactorum; the detection method can be applied to high-sensitivity rapid detection of the phytophthotacactorum; simultaneously, pathogens can be identified in initial stage of invasion of diseases, and the phytophthotacactorum soil of a field can be detected. The LAMP detection primer composition for the phytophthotacactorum, as well as the LAMP detection kit and the LAMP detection method of the LAMP detection primer composition, disclosed by the invention, play an important role in epidemic treatment caused by the phytophthotacactorum, reduction of sightless usage of pesticides, reduction of production cost and reduction of environmental pollution caused by the pesticides as well.

The invention discloses a method for molecular detection of Phytophthora cambivora(Petri)Buisman with the loop-mediated isothermal amplification (LAMP) technique based on color differentiation, and primers. The sequences of the primers are shown from SEQ ID NO.1 to SEQ ID NO.6. According to the detection system, under the isothermal condition of 62 DEG C, Phytophthora cambivora(Petri)Buisman can be detected quickly, conveniently and efficiently with high specificity and sensitivity, no complicated instrument is needed, a new technical platform is provided for detection of Phytophthora cambivora(Petri)Buisman, and the requirement for field detection of Phytophthora cambivora(Petri)Buisman can be well met. The detection system is suitable for inspection and quarantine of entry/exit plants and plant products, and investigation, rapid diagnosis and monitoring of diseases, and is of great importance in preventing Phytophthora cambivora(Petri)Buisman from entering China. Meanwhile, the establishment of the system provides technical guidance and theoretical basis for detection of other pathogenic bacteria.

The invention discloses Phytopythiumvexans detection primers, LAMP detection system, kit and method. The detection primers include a forward inner primer FIP, a backward inner primer BIP, a forward outer primer F3, a backward outer primer B3 and a forward loop primer LF. Compared with a traditional detection technology for identifying Phytopythiumvexans according to morphological characteristics,the detection method provided by the invention has the advantages of high accuracy and sensitivity and convenience in operation, and provides a new technical platform for Phytopythiumvexans detectionwhile realizing constant-temperature amplification. The method can be used for high-sensitivity rapid detection of the Phytopythiumvexans in production practice, pathogens can be identified in the early stage of disease infection, and the Phytopythiumvexans in field soil can be detected. According to the invention, reliable technical and theoretical bases are provided for prevention and treatmentof the Phytopythiumvexans.

The invention discloses a primer combination for detecting Phialophora gregata by loop-mediated isotherm amplification technique and application thereof. The primer combination is composed of a forward outer primer F3 shown in SEQ ID NO.1, a reverse outer primer B3 shown in SEQ ID NO.2, a forward inner primer FIP shown in SEQ ID NO.3, a reverse inner primer BIP shown in SEQ ID NO.4, a loop primer LF shown in SEQ ID NO.5, and a loop primer LB shown in SEQ ID NO.6. The primer combination can be applied to preparation of a Phialophora gregata loop-mediated isotherm amplification detection kit. The kit has good specificity and sensitivity, has high amplification speed, has high efficiency, can identify conveniently, can meet the present crying need for rapid detection of the Phialophora gregata, can be used for quarantine of imports and exports, field quarantine and other field testing, and can be easily promoted and applied in a large range.

The invention discloses a method for fast detecting botryis cinerea's resistance to QoI type bactericides and a primer composition. The method is based on the loop-mediated isothermal amplification technology. The LAMP detecting primer composition comprises a forward inner primer FIP, a backward inner primer BIP, a forward outer primer F3 and a backward outer primer B3. The method has the advantages that the method is simple and convenient, good in practicality, high in sensitivity, high in specificity and high in accuracy, isothermal amplification is realized, a new technical platform is provided to the detection of the botryis cinerea's resistance to the QoI type bactericides, the QoI type bactericide resistance groups of botryis cinerea can be monitored, and the development trend of the resistance groups can be learned about timely; the method has important practical significance to manage crop grey mold resistance, reduce pesticide environmental pollution and lower agricultural production cost.

The present invention discloses a novel target Phibe_s00003g00002.1 for specifically detecting phytophthora hibernalis, and a specific detection primer of the novel target and a detection method. Thedetection target Phibe_s00003g00002.1 has a DNA sequence as shown in SEQ ID NO: 1, and a protein sequence as shown in SEQ ID NO: 2. Meanwhile, the invention discloses a combination of a specific primer and a probe for RPA detection technology based on the specific detection target Phibe_s00003g00002.1. A forward primer sequence of the specific primer is shown in SEQ ID NO: 3, a reverse primer sequence of the specific primer is shown in SEQ ID NO: 4, and a probe sequence is shown in SEQ ID NO: 5. According to the invention, the novel detection target is discovered. The rapid detection method based on the target has high accuracy, high specificity, convenient operation and good practicability, and achieves constant temperature amplification. Meanwhile, the combination of the primer and the probe designed by the invention is used for RPA-LFD detection technology, has high specificity and high sensitivity, and provides a new technical platform for the detection of phytophthora hibernalis.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) combined detection primer, a detection kit and a detection method for rhizome traditional Chinese medicinal materials. The LAMP combined detection primer comprises a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a forward loop primer LF and a reverse loop primer LB; the detection kit comprises an LAMP detection primer and an LAMP reaction solution. The detection method has high accuracy and sensitivity and is convenient to operate, isothermal amplification is achieved, and meanwhile a new technical platform is provided for detection of common root rot and southern blight pathogenic bacteria. The kit can be used for high-sensitivity rapid detection of common root rot and southern blight pathogenic bacteria of traditional Chinese medicinal materials in production practice, can detect pathogenic bacteria in the early stage of disease infected plants, and can detect pathogenic bacteria in field soil. Reliable technical and theoretical basis is provided for prevention and treatment of common root rot and southern blight of rhizome traditional Chinese medicinal materials (such as salvia miltiorrhiza, radix aconiti carmichaeli and rhizoma corydalis).