LAMP (loop-mediated isothermal amplification) combined detection primer, detection kit and detection method for rhizome traditional Chinese medicinal materials

Abstract

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) combined detection primer, a detection kit and a detection method for rhizome traditional Chinese medicinal materials. The LAMP combined detection primer comprises a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a forward loop primer LF and a reverse loop primer LB; the detection kit comprises an LAMP detection primer and an LAMP reaction solution. The detection method has high accuracy and sensitivity and is convenient to operate, isothermal amplification is achieved, and meanwhile a new technical platform is provided for detection of common root rot and southern blight pathogenic bacteria. The kit can be used for high-sensitivity rapid detection of common root rot and southern blight pathogenic bacteria of traditional Chinese medicinal materials in production practice, can detect pathogenic bacteria in the early stage of disease infected plants, and can detect pathogenic bacteria in field soil. Reliable technical and theoretical basis is provided for prevention and treatment of common root rot and southern blight of rhizome traditional Chinese medicinal materials (such as salvia miltiorrhiza, radix aconiti carmichaeli and rhizoma corydalis).

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a combination detection primer, a detection kit and a detection method of rhizome-like Chinese medicinal materials LAMP. Background technique [0002] Fusarium oxysporum belongs to Eumycetes, Ascomycota, Sordariomycetes, Hypocreales, Nectriaceae, Fusarium . It is a kind of facultative parasitic fungus that can infect plants and survive in the soil. It is the pathogenic bacteria of common root rot in Chinese medicinal materials (Aconite, Yuanhu, Salvia, etc.). And the plant wilt caused by its infection is a worldwide soil-borne fungal disease. It has a wide range of hosts and can cause more than 100 types of plant wilts such as melons, Solanaceae, bananas, cotton, legumes and flowers. The pathogen damages plants from the roots, causes vascular bundle diseases, and causes plant death. It can occur during the whole growth period of plants, causing huge losses to productio...

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Problems solved by technology

[0008] The purpose of the present invention is to provide a combination detection primer, detection kit and detection method of LAMP of rhizome Chinese medicinal materials, and aim at the detection and identification of root rot and white silkworm pathogens of rhizome Chinese medicinal materials in the prior art mainly based on morphological characteristics , the method takes a long time, the procedure is cumbersome, highly technical, and the accuracy is low. It is difficult to monitor the occurrence of the disease in time and control the spread and prevalence of pathogenic bacteria. In addition, the existing PCR molecular detection needs to rely on expensive instruments such as amplifiers, and For problems such as long detection time, a rapid molecular detection method is provided for root rot of rhizome Chinese medicinal materials and pathogenic bacteria of white silkworm. LAMP detection is performed on pathogenic bacteria. The detection cycle is short, the accuracy is high, the sensitivity is high, and the detection results are observed by naked eyes.

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