Primer combination for detecting succulent plant stem fusarium moniliforme based on LAMP (Loop-mediated Isothermal Amplification) and application thereof

A succulent and primer combination technology, which is applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of long detection time, cumbersome procedures, low accuracy, etc., and achieve reliable results and sensitivity. High and strong specificity
CN108977508AInactive Publication Date: 2018-12-11INST OF PLANT PROTECTION FAAS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION FAAS
Publication Date
2018-12-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a primer combination for detecting succulent plant stem fusarium moniliforme based on LAMP (Loop-mediated Isothermal Amplification) and application thereof. The LAMP primer combination for detecting the succulent plant stem fusarium moniliforme consists of 1 pair of outside primers F3 / B3 and a pair of inside primer FIP / BIP, wherein the F3 / B3 and FIP / BIP primer sequences areshown as SEQ ID NO. 1 to 4. By adopting the detection system disclosed by the invention, the succulent plant stem fusarium moniliforme can be detected rapidly, conveniently and efficiently at high specificity and high sensitively through incubation at the constant temperature of 65 DEG C for 1 hour without complex and expensive instruments, and in-situ detection of the succulent plant stem fusarium moniliforme can be met well. The detection system can be widely applied to early diagnosis of field diseases of establishment units as well as monitoring and identification of viruses, and a reliable technological and theoretical basis is provided for prevention and control of the succulent plant stem fusarium moniliforme.
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Description

technical field

[0001] The invention belongs to the technical field of disease detection, identification and prevention of horticultural crops, and in particular relates to a primer combination and application thereof for loop-mediated isothermal amplification (Loop-mediated Isothermal Amplification, LAMP) detection of succulent stem rot pathogens, which can be used for succulent stem rot The rapid, sensitive and specific molecular detection of rot pathogen can be used for early diagnosis and monitoring identification of succulent stem rot. Background technique

[0002] Succulentsrefers to higher plants with hypertrophied plant vegetative organs, usually with three vegetative organs of roots, stems, and leaves and three reproductive organs of flowers, fruits, and seeds. In gardening, it is also called succulent plant or succulent flower, but the name succulent plant is the most commonly used. Succulents are popular all over the world as potted plants, and most of the common...

Claims

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