Culture medium for composite enrichment of salmonella, Vibrio parahaemolyticus and Vibrio cholerae, and preparation thereof
A technology for Vibrio hemolyticus and Vibrio cholerae, which is applied in biochemical equipment and methods, determination/inspection of bacteria and microorganisms, etc., can solve the problem that the culture medium is not selective, the detection efficiency is low, and it does not conform to the development trend of pathogenic bacteria. and other problems to achieve the effect of shortening the enrichment time
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Embodiment 1
[0020] Weigh 20.1g of buffered peptone water, 1.25g of glucose, 1.25g of mannitol, 0.05g of sodium pyruvate, 1.25g of anhydrous sodium sulfite, 5.0g of sodium citrate, 15.0g of sodium chloride, 2.5g of bile salt, and 1000ml of distilled water and heat Sterilize at 121°C for 18 minutes after melting. After the culture cools down to below 50°C, add 1.0 mg of potassium tellurite under sterile conditions, dissolve and mix well, and distribute for use.
[0021] Three target bacteria Salmonella, Vibrio parahaemolyticus and Vibrio cholerae and non-target bacteria Escherichia coli, Bacillus cereus, Yersinia enterica, Proteus, Enterobacter aerogenes, Shigella flexneri, Abalone Slant cultures of Shigella aeruginosa, Pseudomonas aeruginosa, Vibrio vulnificus, Vibrio alginolyticus will be diluted to 10 -4 Add 0.2ml of each target bacterium and non-target bacterium solution into the above-mentioned medium, and incubate at 37°C for 24h. The OD value at 540 nm was measured with a visible s...
Embodiment 2
[0027] Weigh 10.05g of buffered peptone water, 1g of glucose, 1g of mannitol, 0.03g of sodium pyruvate, 0.5g of anhydrous sodium sulfite, 1.0g of sodium citrate, 5.0g of sodium chloride, 1g of bile salt, and 1000ml of distilled water after heating and melting for 123 Sterilize at ℃ for 16 minutes. After the culture cools down to below 50°C, add 0.5 mg of potassium tellurite under aseptic conditions, dissolve and mix well, and distribute for use.
[0028] Three target bacteria Salmonella, Vibrio parahaemolyticus and Vibrio cholerae and non-target bacteria Escherichia coli, Bacillus cereus, Yersinia enterica, Proteus, Enterobacter aerogenes, Shigella flexneri, Abalone Slant cultures of Shigella aeruginosa, Pseudomonas aeruginosa, Vibrio vulnificus, Vibrio alginolyticus will be diluted to 10 -4 Add 0.2ml of each target bacterium and non-target bacterium solution into the above-mentioned medium, and incubate at 37°C for 24h. The OD value at 540 nm was measured with a visible spe...
Embodiment 3
[0034] Weigh 30.15g of buffered peptone water, 5.0g of glucose, 5.0g of mannitol, 0.05g of sodium pyruvate, 2.5g of anhydrous sodium sulfite, 5.0g of sodium citrate, 25.0g of sodium chloride, 5g of bile salt, and 1000ml of distilled water and heat to melt Then sterilize at 125°C for 15 minutes. After the culture cools down to below 50°C, add 2.5 mg of potassium tellurite under sterile conditions, dissolve and mix well, and distribute for use.
[0035] Diluted into three target bacteria Salmonella, Vibrio parahaemolyticus and Vibrio cholerae and non-target bacteria Escherichia coli, Bacillus cereus, Yersinia enterica, Proteus, Enterobacter aerogenes, Shigella flexneri bacteria, Shigella baumannii, Pseudomonas aeruginosa, Vibrio vulnificus, Vibrio alginolyticus slant culture, will be diluted to 10 -4 Add 0.2ml of each target bacterium and non-target bacterium solution into the above-mentioned medium, and incubate at 37°C for 24h. The OD value at 540 nm was measured with a visi...
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