Novel specific molecular targets for identifying salmonella and rapid detection method thereof

Abstract

The invention provides a group of molecular targets for detecting salmonella. The molecular targets comprise nucleotide sequences as shown in SEQ ID NO. 1-13. The invention also provides a group of primers for detecting the salmonella molecular targets in the claim 1, and the nucleotide sequences of the primers are shown as SEQ ID NO.14-39. The primers are adopted to perform PCR amplification on the specific targets, whether a sample contains salmonella or not is judged by detecting whether an amplification product contains a target strip or not, the detection cost is reduced, and the method is more practical; the specific molecular targets, namely 13 conservative nucleic acids, have single specificity, the detection result is specific, and the result judgment is simple.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows salmons bacteria like SARS-CoV-2 (severe respiratory syndrome coronavirus) to detect by specifically binding certain DNA sequences called CRISPRs or similar small genetic material onto their surface proteins. These specialized probing agents are designed to bind only one type of virus - they cannot harm other organisms' cells. By doing this, it provides an effective way to identify different types of viruses quickly and accurately while reducing costs associated therewith compared to previous testing techniques such as blood culture.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding novel molecules from samples containing Salmarchiaceae or related genera like Sarcophaga gut flora, particularly those associated with severe illnesses such as gastroenteritis epidemias. Current diagnostic techniques involve testing each sample separately but they may also result in misdiagnoses because different tests only identify certain types rather well at once. There is currently lacked effective ways to quickly and accurately determine whether any particular type of organism causes disease without having multiple testings.

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