Primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, kit and detection method thereof

A Salmonella and Shigella technology, applied in the field of food microbial safety testing, can solve problems such as not being able to fully meet the national food safety standards, and achieve the effects of wide promotion and application market prospects, low equipment requirements, and strong specificity

Active Publication Date: 2016-02-17
COFCO NUTRITION & HEALTH RES INST +1
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  • Description
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  • Application Information

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Problems solved by technology

It can be seen that the existing technology cannot fully meet the needs of national food safety standards

Method used

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  • Primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, kit and detection method thereof
  • Primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, kit and detection method thereof
  • Primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, kit and detection method thereof

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Embodiment Construction

[0052] According to some embodiments of the present invention, in addition to the primer set for multiplex PCR detection of the present invention, the kit for multiplex PCR detection of the present invention may further include components such as reaction buffer, 4 kinds of deoxynucleoside triphosphates, DNA polymerase, etc. .

[0053] According to some preferred embodiments of the present invention, in addition to the primer set for multiplex PCR detection of the present invention, the kit for multiplex PCR detection of the present invention may further include a PCR reaction base solution with the following components: Tris-HCl; KCl; MgCl 2 ; dATP, dTTP, dGTP, and dCTP; and Taq DNA polymerase.

[0054] According to some more preferred embodiments of the present invention, in the multiplex PCR detection kit of the present invention, each component is contained in the final PCR reaction solution of 25 μL at the following concentration:

[0055]

[0056] Primer pair for inv...

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Abstract

The invention is used for the field of food microorganism safe detection, and provides a primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, a kit and a detection method thereof. The primer group used for the multiple PCR detection, the kit and the detection method can combine and use species-specificity discrimination gene and drug resistance-related gene, and the technical effects of wide detection cover scope, strong specificity and high sensitivity can be realized, detection time consumption is short, operation is simple, equipment requirement is low, flux is high; manpower, material resource and financial resource can be saved, the primer group used for the multiple PCR detection, the kit and the detection method are suitable for rapid detection requirement, and are easily popularized and applied in practical production.

Description

technical field [0001] The invention belongs to the field of food microbe safety detection, and relates to a primer set, a kit and a method for rapidly detecting and identifying pathogenic bacteria in food by using multiple PCR technology. Specifically, the present invention relates to a multiplex PCR detection primer set, kit and detection method for Salmonella sp. and Shigella sp. in food. Background technique [0002] In recent years, food safety incidents have occurred frequently, and food safety issues have become more and more concerned and valued by the public. Many food-borne pathogens are transmitted through water and food and cause food-borne diseases, and their morbidity and mortality are gradually increasing. Worldwide, more than 30% of the population is infected with foodborne illness each year, costing billions of dollars. Preventing and controlling the occurrence and spread of foodborne diseases has become one of the important measures to solve social food s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/42C12R1/01
Inventor 李慧蔡军刘洋欧竞堃石嵩杨凯
Owner COFCO NUTRITION & HEALTH RES INST
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