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91 results about "Salmonella bareilly" patented technology

Salmonella Bareilly is an unusual serotype of Salmonella. Public health investigators used DNA “fingerprints” of Salmonella bacteria obtained through diagnostic testing with pulsed-field gel electrophoresis, or PFGE, to identify cases of illness that may be part of this outbreak.

Oligonucleotide probe kit for detecting common intestine trac kpathogenic bacteria and its use

The present invention belongs to the field of microbe detecting technology. The oligonucleotide probe for detecting common intestinal tract pathogenic bacteria is designed on 16S rRNA and 23S rRNA of bacteria, ipaH of dysentery bacillus giant plasmid, VipR of Salmonella typhi and other gene sequence, has length of 25-50 bp, and relatively high sensitivity and specificity. The oligonucleotide probe is suitable for detection based on nucleic acid hybridization principle, especially detection based on gene chip principle. Under certain use condition, it can detect Listeria, parahemolutic vibrio, Campylobacter, etc. It may be used in many aspects, such as disease diagnosis, environment detection, food poisoning detection, etc.
Owner:RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA

Gene chip of main pathogenic microorganism in drinking water and testing kit

The invention provides a gene chip of main pathogenic microorganism in drinking water and a testing kit, which mainly aims at 11 kinds of bacteria of colibacillus / Shigella, salmonella, vibrio cholera, vibrio parahaemolyticus, staphylococcus aureus, enterococcus faecails, pseudomonas aeruginosa, legionella pneumophilia, pneumobacillus, yersinia enterocolitica and the like, and L.interrogans. The gene chip comprises a solid phase carrier and a oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe contains gyrB gene with tremendous evolutionary advantage, ITS gene and DNA segment selected from 16srRNA gene or complementary DNA segment. The gene chip and the testing kit of the invention can test the main pathogenic microorganism in drinking water, and has the characteristics of simple operation, high throughput, high accuracy, strong repeatability and the like, and can be used for clinical test for the water quality monitoring department.
Owner:NANKAI UNIV

Culture medium for composite enrichment of salmonella, Vibrio parahaemolyticus and Vibrio cholerae, and preparation thereof

The invention relates to a complex enrichment medium used for salmonella, vibrio parahaemolyticus and comma bacillus and a method for preparing the same. The formulation components of the complex enrichment medium in weight portion are as follows: 0.5 to 1.5 portions of buffer peptone water, 1000 portions of distilled water, 1.0 to 5.0 portions of glucose, 1.0 to 5.0 portions of mannitol, 0.03 to 0.06 portion sodium pyruvate, 0.5 to 2.5 portions of anhydrous sodium sulfite, 1.0 to 10.0 portions of sodium citrate, 5.0 to 25.0 portions of sodium chloride, 1 to 5 portions of cholate, and 0.0005 to 0.0025 portion of potassium tellurite. The method comprises the following steps: firstly, adding the buffer peptone water and the like into the distilled water, and sterilizing the mixture after heating and melting; and secondly, cooling the mixture to 50 DEG C, and then adding the potassium tellurite into the mixture to be mixed evenly. The complex enrichment medium can integrate two steps of primary enrichment and selective enrichment of bacterial detection to shorten the enrichment time to 24 hours, can perform enrichment on three target pathogens at the same time, and can inhibit the growth of other microorganisms.
Owner:SOUTH CHINA UNIV OF TECH

Cross-protective salmonella vaccines

The present invention relates to a method of protecting pigs against disease caused by infection by heterologous serotypes of Salmonella including but not limited to S. typhimurium comprising administering to the pigs a modified live vaccine incorporating S. cholerasuis.
Owner:INTERVET INT BV

Gene chip for detecting 11 types of common infectious diarrheal disease pathogen and application thereof

The invention relates to a gene chip and an application, which belongs to the biological detection field. The invention designs a set of detection probes by aiming at 11 types of common infectious diarrheal disease pathogen microorganism in clinic, and the gene chip containing the set of the probe. The detection probes comprises a vibrio parahaemolyticus probe, a vibrio vulnificus, a vibrio cholera probe, a vibrio alginolyticus probe, a vibrio furnissii probe, a shigella probe, an escherichia coli probe, an aeromonas probe, a salmonella probe, a campylobacteria probe, and a proteusbacillus vulgaris probe; the above mentioned probe sequence is shown as SEQ ID No: 1-117. The gene chip and probe can detect 11 types of common infectious diarrheal disease pathogen, the detection flux is high, the specialty is strong, the sensitivity is high, and the detection is rapid and effective.
Owner:YANTAI YUHUANGDING HOSPITAL

Multi-PCR fast detecting reagent kit for salmonella and bacillus coli 0157 in livestock and poultry meat and testing method thereof

The invention relates to a microbial detection device and a detection method of meat of livestock and poultry. The salmonella and Escherichia coli O157 multiple PCR rapid detection kit of the meat of livestock and poultry includes: 10 multiplied by PCR buffer, 1.0 to 3.0mmol / L of MgCl2, 240 Mumol / L of dNTP each, 200nmol / L of salmonella primer, 200nmol / L of Escherichia coli O157 primer and 1.3 to 3.0U of Taq enzyme. The microbial detection device has better specificity, which can complete the simultaneous detection of the salmonella and the Escherichia coli O157 within 8 to 9h; compared with the conventional bacteriology, the diagnosis is rapid, convenient and economical, thus providing a powerful technical means for the diagnosis of pathogen in a food sample and having good application prospect.
Owner:GUANGDONG INST OF MICROORGANISM

Gene chip for detecting six kinds of diarrhea pathogens and its prepn process and kit

The present invention relates to one kind of gene chip for detecting six kinds of diarrhea pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The oligonucleotide probe includes DNA or cDNA segments selected from nucleotide sequences corresponding to the genomes of Shigella, haemorrhagic colibacillus, invasive colibacillus, Vibrio parahaemolyticus, Vibrio cholreae and salmonella. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency and high detection accuracy.
Owner:IPE BIOTECHNOLOGY CO LTD

Method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time

The invention provides a method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time, including the steps: collecting specific pathogenic gene or toxin gene of the target pathogen and using it as a target gene to design primers and probes so as to make the reaction conditions consistent; extracting a genome template of a sample to be detected; adding the template respectively into tubules equipped with different specific upstream and downstream primers and probes, and then adding the corresponding fluorescent quantitative PCR reagents; under the same cycle of fluorescent quantitative PCR, the corresponding primers and probes are used to detect the samples simultaneously, quickly and quantitatively in their respective reaction tubes. Easier, Quick and efficient, Twelve common pathogenic bacteria (Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Streptococcus betae, Yersinia enterocolitica, Streptococcusfaecalis, Shigella, Proteus mirabilis, Vibrio fluvialis, Campylobacter jejuni, Staphylococcus aureus) can be detected simultaneously in drinking water and food economically.
Owner:INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI

Detection genetic chip and detection kit for infectious diarrhea

The invention provides a genetic chip and a detection kit related to major pathogenic microorganisms causing infectious diarrhea of human beings, which are mainly specific to 13 types of bacteria including lapactic bacillus coli / shigella, salmonella, comma bacillus, vibrio parahemolyticus, aeromona hydrophila, plesiomonas, virio hollisae, vibrio fluvialis, vibrio mimicus, Wauter's strains, vibriodamsela and vibrio furnissii. The genetic chip comprises a solid-phase carrier and an oligonucleotide probe fixed on the solid-phase carrier, wherein the oligonucleotide probe comprises DNA (Deoxyribonucleic Acid) fragments selected from genes including a gyrB gene, an ITS gene, a dnaJ gene, a toxR gene and the like with remarkable biological evolution advantages or complementary DNA fragments. By adopting the genetic chip and the detection kit provided by the invention, major pathogenic microorganisms causing infectious diarrhea of human beings can be detected. The genetic chip and the detection kit have the characteristics of easiness and convenience for operation, high flux, high accuracy, high repeatability and the like, and can be applied to clinical detection of inspection departments in hospitals.
Owner:NANKAI UNIV

Gene chip and applications thereof in detection of aquatic pathogenic microorganism

The invention relates to a gene chip in the detection of aquatic pathogenic microorganism, a preparation method and applications thereof in the detection of aquatic pathogenic microorganism. A conserved sequence selecting from a target pathogenic microorganism gene is fixed on a solid-phase carrier as an oligonucleotide probe, in the proper hybridization condition, a marked sample to be detected is added for reaction, and the corresponding aquatic pathogenic microorganism can be identified according to a probe display signal on the gene chip. The gene chip can fast detect the aquatic pathogenic microorganism such as aeromonas hydrophila, edwardsiella tarda, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, salmonella typhimurium, shigella and the like, thus being high in detection sensitivity, and strong in specificity and greatly shortening the detection period.
Owner:HANGZHOU ACAD OF AGRI SCI

Composite gene chip for food-borne pathogenic bacteria detection

The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.
Owner:广东省疾病预防控制中心

Multiple-PCR detection method for pathogenetic bacteria in soil

The invention discloses a multiple-PCR detection method for pathogenetic bacteria in soil, which is characterized in that: multiple PCR is adopted to simultaneously amplify specific genes of enteropathogenic E. Coli, salmonella, Staphylococcus aureus, pseudomonas aeruginosa and Shigella flexneri for one time, namely phoA, ttrBCA, vicK, ecfX, ipaH and virA, and then PCR amplification products are detected by agarose gel electrophoresis. The multiple-PCR detection method overcomes defects of complicated operation, time and labor consumption and the like of the conventional pathogenetic bacteria detection technology, fully applies the multiple-PCR detection technology in detecting the pathogenetic bacteria in the soil, increases the types of the pathogenetic bacteria and number of pairs of primers as possible, and improves the specificity and sensitivity of the detection.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI

Salmonella, Listeria monocytogenes, bacillus coli multiple PCR rapid detection kit and uses thereof

The invention discloses a method simply and rapidly detecting Salmonella, Listeria monocytogenes producing monocyte, and escherichia coli simultaneously and a detection reagent kit invented based on the method. Firstly the DNA templates of the three microorganisms are extracted through hot dehiscing (Salmonella, escherichia coli) and enzymolysis method (Listeria monocytogenes), then the specific primers according to salmonella, Listeria monocytogenes producing monocyte, and escherichia coli are designed respectively, the practical amplification fragments are salmonella 320bp, Listeria monocytogenes producing monocyte 440bp, and escherichia coli 706bp. The invention has the advantages of celerity and nicety compared with the bacteria separation, identification of classical method and serological typing test method, strong specificity and high sensitivity compared with the generic multiplex PCR method, and the ability to detect and identify the three pathogens simultaneously.
Owner:CHINA AGRI UNIV

Primer, reagent box and method for detecting salmonella and integron (int)

The invention discloses primer, a reagent box and a method for detecting salmonella and integrin (int). The primer comprises upstream primer SLMF: TGCGTCAGGACCTCATAG, downstream primer SLMR: ATGCCTGGAGGAGTGTTT, upstream primer intF: TTTACGCTGCTGTATGGT, and downstream primer intR for detecting int: TTATTGCTGGGATTAGGC. The reagent box is filled with 10 uL of HRM reaction premix liquid, 0.2-3.4 uL of primer, 1 uL of DNA template and is filled into 20 uL by water. The detecting method includes extracting sample DNA and performing PCR amplification. After amplification, products are subjected to high-resolution melting curve analysis, and results are judged by an amplification curve. The reagent box is reasonable in component and proportion, is convenient to use and quick and accurate to detect, the method is convenient and quick to operate, and low in cost, and detecting results are accurate.
Owner:蔡先全

Method for detecting food-derived pathogenic enterobacteria by composite fluorescence PCR technique

The invention discloses a method by using composite fluorescence PCR technique to detect food-borne pathogenic enterobacter and pertains to bacteria detection technical field. The main technic proposal is to design a primer group sequence. The pathogenic enterobacter is common pathogenic bacteria in food and imposes a serious thread to human health. The quick and accurate detection of pathogenic enterobacter in food is a main premise condition for effective prevention and control of pathogen bacteria infection. The food-borne pathogenic enterobacter required detection mainly comprises shigella, salmonella, Yersinia enterocolitica, hemorrhagic scherichia coli and scherichia coli O157: H7. The invention overcomes technical shortage in the prior art aiming at the object bacteria and provides the quick and low-cost detection method by using composite fluorescence PCR technique to detect shigella, salmonella, Yersinia enterocolitica, hemorrhagic scherichia coli and scherichia coli O157: H7. The method can use two-diode PCR reaction and primarily screen shigella, salmonella, Yersinia enterocolitica, hemorrhagic scherichia coli and scherichia coli O157: H7.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1

Double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) method for detecting listeria in food on basis of monoclonal antibodies

The invention discloses a double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) method for detecting listeria in food on the basis of monoclonal antibodies, belonging to the technical field of immunoassay. The method comprises the following steps: immunizing 8-week BALB mouse with prokaryotically expressed listeria monocytogenes P60 protein, and obtaining 15 strains of listeria specific monoclonal antibodies by immunization, fusion and multiple screening; respectively marking horse radish peroxidase HRP, and carrying out pairwise coupling by taking the listeria monocytogenes CMCC54003 as targets. The sandwich ELISA method which is established by screening different paired cross reactions and taking 2G7 as a coating antibody and 2H8-HRP as an enzyme labelled antibody has cross reactions to listeria including the listeria monocytogenes, and has no cross reactions to tested staphylococcus aureus, escherichia coli, escherichia coli O157, salmonella, enterobacter sakazakii, campylobacter jejuni and campylobacter coli. According to the method disclosed by the invention, the monoclonal antibodies having listeria specificity are prepared and the double-antibody sandwich ELISA method is established, and the method provides a technical means for specific rapid detection of the listeria in food.
Owner:JIANGNAN UNIV

Kit and detection method for detecting salmonellae by combining recombinase isothermal amplification and immune colloidal gold test strip

The invention discloses a kit for detecting salmonellae by combining recombinase isothermal amplification and an immune colloidal gold test strip. The kit comprises a salmonella gene primer group DNA,a recombinase isothermal amplification skit, sterile distilled water and a nucleic acid immune colloidal gold test strip. The kit is convenient to operate and quite applicable to field detection; thedetection cost is low, and only 20min is required by the whole detection process; the sensitivity is 104 times higher than a limit of detection implemented by combining PCR (polymerase chain reaction) and agarose gel electrophoresis, so that the kit can be widely used for detecting various pathogenic bacteria, and the salmonellae can be detected accurately, sensitively and rapidly.
Owner:中科智测(天津)科技有限公司

Universal primers and probe for on-site rapid detection of Brucella and kit

The invention discloses universal primers and a probe for on-site rapid detection of Brucella and a kit. The forward primer sequence is shown as SEQIDNo.1, the reverse primer sequence is shown as SEQIDNo.2, and the probe sequence is shown as SEQIDNo.3. The universal primers, the probe and the kit provided by the invention for detection of Brucella have high sensitivity and strong specificity, minimumly can detect 6.0*10<0>cfu Brucella, and have no cross reaction with Escherichia coli O157:H7, yersinia enterocolitica O:9, Salmonella, Staphylococcus aureus and other bacteria. The universal primers, the probe and the kit provided by the invention not only can be used for detection of Brucella strains, but also can be used for detection of clinical samples, which mainly include blood, milk samples, aerosol samples, and tissue samples, etc. The kit provided by the invention is convenient to use, has no need for special equipment, can carry out sensitive, specific and rapid detection of Brucella on the crude split product of a to-be-detected sample in 25min just at 38DEG C, and is suitable for field or grassroots brucellosis quarantine work.
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI

Primer combination for detecting infectious diarrhea pathogen and kit thereof

The invention relates to a primer combination for detecting infectious diarrhea pathogen. The primer combination comprises at least one combination of the following combinations: a rotavirus primer combination, an enteric adenovirus primer combination, a Norovirus primer combination, a salmonella primer combination, a Shigella primer combination and a campylobacter jejuni primer combination. The invention also relates to a kit containing the primer combination. The kit also comprises a primer-coating micro-fluidic chip, an isothermal amplification reaction liquid, an isothermal amplification enzyme solution, and a negative control. The invention also relates to a detection method by using the above primer combination. The detection method comprises steps as follows: coating of the primer combination, nucleic acid extraction of a sample to be detected, LAMP reaction and result interpretation. The kit provided by the invention can detect infectious diarrhea pathogen rapidly and accurately within one hour, and is also of great significance for rapid assisted guide treatment and drug use. The multi-index detection also can be used in regional epidemiological investigation and epidemic surveillance so as to study epidemic situation of infectious diarrhea in China.
Owner:SHANGHAI IGENETEC DIAGNOSTICS CO LTD

Preparation method of colloidal gold test paper strip for detecting salmonella in food based on salmonella core polysaccharide monoclonal antibody

The invention provides a preparation method of a colloidal gold test paper strip for detecting salmonella in food based on salmonella core polysaccharide monoclonal antibody SQX6D8, and belongs to the field of immunological analysis. According to the method, a Salmonella typhimurium lipopolysaccharide (LPS) and bovine serum albumin (BSA) conjugate synthesized through a sodium periodate method is used as the coating antigen of the test line (T line) of the colloidal gold test paper strip, and salmonella core polysaccharide monoclonal antibody SQX6D8 is used as the gold-labeled antibody. According to the present invention, the principle of the method is different from the principle of the conventional pathogenic bacteria colloidal gold test paper strip sandwich method, the indirect competition principle is used to detect, it is ensured that the cross-reaction is provided for the intragenus salmonella while no cross-reaction is provided for the extragenus salmonella through the salmonella core polysaccharide specific monoclonal antibody, and the analysis means is provided for the complete, convenient and rapid detection of the salmonella in the food.
Owner:JIANGNAN UNIV

Salmonella and/or e. coli enzyme test and/or liquid drop test for biological substances, on non-human samples

A test kit and test apparatus are provided for the detection of enzymes excreted by broken or ruptured bacteria cells in various environments, such as in the field, soil extracts, produce washing, slaughter houses, food preparation area's and other related or non-related areas where a quick test or rapid test is needed to obtain qualitative results. The method utilizes various components which allow for the collection of a sample from suspected contaminated areas. One method is to collect a sample from a pond or other water source possibly contaminated and by using a clean lab sample tube or clean plastic bag, a field hand mixer, a buffer, a pipette and a test strip with a reagent pad.
Owner:LOWENKAMP JR WILLIAM

Attenuated vector bacterium of salmonella choleraesuls and construction method of attenuated vector bacterium

InactiveCN106754594AReduce usageHas attenuated safety featuresBacteriaMicroorganism based processesBacteroidesAntibiotic Y
The invention provides an attenuated vector bacterium of salmonella choleraesuls and a construction method of the attenuated vector bacterium and belongs to the technical field of animal bacterium genetic engineering. The attenuated vector bacterium is C78-3 salmonella choleraesuls without deltamanA, deltacrp::TT araC PBAD, deltarelA:: araC PBAD lacI TT, deltasoB and deltaasdA genes and is named as rSC0016. The method for taking a suicide vector without a resistance marker as a salmonella choleraesuls constructing vector is adopted and the utilization of the vector marked with antibiotics can be avoided; the vector can be safely used in clinical practice; furthermore, a balanced lethal system carries an exogenous antigen and can guarantee that only vaccine strains carrying the exogenous antigen can be used for producing vaccines and enter a host and survive, so that the immunity efficiency of the vaccines is improved.
Owner:YANGZHOU UNIV

Detection kit of 16 food born pathogenic bacteria

The invention discloses a detection kit of 16 food born pathogenic bacteria. The detection kit comprises a PCR amplification reagent and reference reagents; the PCR amplification reagent is composed of PCR buffer solution A, enzyme system B, and a primer probe mixed solution of the 16 food-borne pathogenic bacteria; the reference reagents are mainly composed of a negative control and a positive control; the PCR buffer solution A contains 5*Buffer, 25mM dN(U)TPs; the enzyme system B is mainly composed of HotStart Hi Taq DNA polymerase and UNG anti-pollution enzyme; the primer probe mixed solution of the 16 food-borne pathogenic bacteria contains a mixed solution of detection primer probes of salmonella, Shigella, enteropathogenic Escherichia coli, enterotoxigenic E. coli, enteroinvasive escherichia coli, adherent escherichia coli, Escherichia coli O157, Staphylococcus aureus, listeria monocytogenes, Bacillus Cereus, campylobacter jejuni, vibrio parahaemolyticus, Vibrio cholerae group O1, Vibrio cholerae group O139, Yersinia enterocolitica, and enterobacter sakazakii. The detection kit is high in detection sensitivity, is simple, and is convenient.
Owner:HANGZHOU ZHUNXIN BIOTECHNOLOGY CO LTD

Synthetic method of immunogen for preparing salmonella cross-type antiboby

The invention discloses a synthetic method of an immunogen for preparing a salmonella cross-type antiboby, and belongs to the technical field of immunoassay. The characteristic that mutant salmonella lipopolysaccharide (Ra-LPS) has no O-specificity side chain is utilized, the EDC and NHS method is adopted for coupling LPS tail end COOH and KLH, and mice sera obtained in an immunization mode have titer on the Ra-LPS and intact LPS. Salmonella thallus peridia of different serogroups are used for detecting the mice sera to obtain the result that all the mice sera have the cross reaction, and it is proved that the immunogen can be used for preparing the salmonella cross-type sera or monoclonal antibody. The synthetic method of the immunogen is used for preparing the salmonella cross-type antiboby, and therefore a solid foundation is laid for detecting the salmonella in the bacterial genus level.
Owner:JIANGNAN UNIV

Preparation method for polyclonal antibody to salmonella effect protein SopB

InactiveCN103204936AGood for western blot analysisFavorable for immunohistochemical analysisHydrolasesSerum immunoglobulinsStainingMicroscopic observation
The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.
Owner:TIANJIN UNIV OF COMMERCE

Dual Tem-PCR quick detection method for salmonella and escherichia coli O78

The invention discloses a dual Tem-PCR quick detection method for salmonella and escherichia coli O78. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, invA gene primer of the salmonella and O-antigen gene primer of the escherichia coli are used for conducting dual Tem-PCR amplification directly on extracted mixing DNA after bacteria enrichment is conducted on a complex sample, and the result is interpreted through gel electrophoresis. A Tem-PCR technology and a dual PCR technology are combined, the problem of enrichment preference of the PCR is solved, the sensitivity degree is improved by 2 to 3 orders of magnitudes compared with an ordinary dual PCR detection, the detection level that the number of sample contaminated bacteria is a single digit can be reached, and the problem that a traditional dual PCR technology is prone to lead to false negative result. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, the salmonella and the escherichia coli O78 can be detected quickly, conveniently, excellently and sensitively, wide-range popularization is easy, the salmonella and the escherichia coli O78 can be used for bacteria identification, disease diagnosis and epidemiological investigation, and wide market prospect and comparatively large economic benefits are achieved.
Owner:QINGDAO KANGLAND BIOTECH

Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium

ActiveCN105039202AReduce inhibitionGood effect of bacteria enrichmentBacteriaMicroorganism based processesNalidixic acidMannitol
The invention relates to the technical field of detection of pathogenic bacterium, and discloses a selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus. The selective medium is prepared from tryptone, peptone, sodium chloride, sodium dihydrogen phosphate, glucose, mannitol, esculin hydrate, sodium citrate, skim milk powder, sterile purified water, potassium tellurite solution, acriflavine solution and nalidixic acid solution. A preparation method of the selective medium comprises the following steps: preparation of potassium tellurite solution; preparation of acriflavine solution; preparation of nalidixic acid solution; preparation of a semi-finished product medium; addition of a sample; and addition of potassium tellurite solution, acriflavine solution and nalidixic acid solution in the medium. The selective medium can carry out multiplex proliferation on the target bacteria and inhibit non-target bacteria, is small in inhibiting effect on the target bacteria in the sub-lethal state so that the target bacteria in the sub-lethal state can realize effective proliferation. The preparation method of the selective medium is easy to operate and high in efficiency.
Owner:舟山出入境检验检疫局综合技术服务中心

Methods and reagents for the detection of salmonella spp

InactiveUS20110151458A1Sugar derivativesMicroorganismsBacteroidesGenus Orthobunyavirus
The invention relates to an in vitro method for the detection of bacteria of the Salmonella spp. genus by means of a quantitative polymerase chain reaction using specific primers for said pathogen from DNA and RNA samples from said microorganism. Said method is useful in the detection of viable and non-viable microorganisms of Salmonella spp. in environmental, clinical and food samples. Likewise, the invention also relates to a kit used for putting said method into practice.
Owner:UNIV DEL PAIS VASCO EUSKAL HERRIKO UNIBERTSITATEA

Multiple rapid detection method, detection primer group and kit for three food-borne pathogenic bacteria

The invention discloses a multiple rapid detection method, a detection primer group and a kit for three food-borne pathogenic bacteria. According to the invention, upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of salmonella correspondingly have sequences as shown in SEQNo.1, SEQNo.2, SEQNo.3 and SEQNo.4; upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of staphylococcus aureus have sequences as shown in SEQNo.5, SEQNo.6, SEQNo.7 and SEQNo.8; upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of Listeria monocytogenes have sequences as shown in SEQNo.9, SEQNo.10, SEQNo.11 and SEQNo.12. By using the method in the invention, the defect that the prior art can not be used for simultaneously judging whether multiple pathogenic bacteria exist in the same system at the same time is overcome, and whether the same detected sample contains one or more of salmonella, staphylococcus aureus and Listeria monocytogenes can be correctly detected.
Owner:浙江省质量技术监督检测研究院

Pair of specific primers for detecting Bergeyella, kit and PCR detection method

The invention discloses a pair of specific primers for detecting Bergeyella, a kit and a PCR detection method. The primers comprise an upstream primer Bergeyella-F: 5'-TTGAAAGCTCCGGCGGATAG-3' and a downstream primer Bergeyella-R: 5'-ACCCTCACGAGAGTAGGTTT-3'. According to the specific primers, the kit and the PCR detection method, streptococcus, bacillus erysipelatos-suis, pasteurella multocida, salmonella, haemophilus parasuis, infectious actinobacillus pleuropneumoniae, stenotrophomonas maltophilia, pig Bergeyella and a Bergeyella zoohelcum 16S rRNA gene sequence are downloaded from a GenBankdatabase; sequence comparison is carried out through MEGA 5.05 software to search a Bergeyella 16S rRNA specific sequence; and Primer-BLAST design is utilized to amplify a pair of specific primers ofBergeyella; and a Bergeyella PCR rapid detection method is established to identify Bergeyella. The method has the advantages of being rapid, convenient, high in sensitivity and strong in specificity.
Owner:HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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