Primer, reagent box and method for detecting salmonella and integron (int)

Abstract

The invention discloses primer, a reagent box and a method for detecting salmonella and integrin (int). The primer comprises upstream primer SLMF: TGCGTCAGGACCTCATAG, downstream primer SLMR: ATGCCTGGAGGAGTGTTT, upstream primer intF: TTTACGCTGCTGTATGGT, and downstream primer intR for detecting int: TTATTGCTGGGATTAGGC. The reagent box is filled with 10 uL of HRM reaction premix liquid, 0.2-3.4 uL of primer, 1 uL of DNA template and is filled into 20 uL by water. The detecting method includes extracting sample DNA and performing PCR amplification. After amplification, products are subjected to high-resolution melting curve analysis, and results are judged by an amplification curve. The reagent box is reasonable in component and proportion, is convenient to use and quick and accurate to detect, the method is convenient and quick to operate, and low in cost, and detecting results are accurate.

Description

technical field [0001] The present invention designs a primer for detecting Salmonella SLM and drug-resistant gene integron int; the present invention also relates to a double fluorescent PCR detection kit comprising the above primers, and the present invention also relates to a method for detecting Salmonella and Methods of drug resistance gene integron int. Background technique [0002] Salmonella, the Latin scientific name salmonella, referred to as SLM, is the main cause of bacterial food poisoning. When humans eat contaminated food, unsterilized or incompletely sterilized food, it may cause food poisoning. In 2008, there was an outbreak of Salmonella in the United States. The main reason for the outbreak is related to the consumption of contaminated tomatoes. In 2009, the United States also recalled beef mince contaminated by Salmonella and E. coli several times, and some vegetable salad products were also recalled due to contamination by E. coli and Salmonella. Accor...

AI Technical Summary

Problems solved by technology

For Salmonella and int-related genes, researchers have carried out research on conventional PCR and fluorescent PCR methods. However, conventional PCR methods require electrophoresis after PCR amplification, which is not only cumbersome to operate, but also easy to cause pollution. It also requires the use of fluorescent fuels that may cause cancer. Although the probe-based fluorescent PCR method is sensitive and accurate, its detection cost is relatively high, and the validity period of the reagent is short, so it is easy to degrade.

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