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Synthetic method of immunogen for preparing salmonella cross-type antiboby

A cross-type antibody, Salmonella technology, applied in the field of immune analysis, can solve the problem that it is difficult to detect Salmonella

Active Publication Date: 2014-09-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since there are more than 2,000 serotypes of Salmonella, the general Salmonella antibody is highly specific due to the limitation of the immunogen, so the established ELISA method is difficult to detect all Salmonella

Method used

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  • Synthetic method of immunogen for preparing salmonella cross-type antiboby

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Experimental program
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Embodiment Construction

[0017] The mutant Salmonella typhimurium lipopolysaccharide Ra-LPS described in the examples was purchased from Sigma.

[0018] The present invention is further illustrated by the following examples.

[0019] instrument:

[0020] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory; KFLOW pure water machine, Kevron Company; ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory; 96-well 8×12 detachable microplate, Xiamen Yi Jiamei Laboratory Equipment Co., Ltd.; MuLtiska Mks microplate reader, Thermo Labsystems; adjustable pipette, Thermo Labsystems; vortex mixer, Shanghai Huxi Instrument Analysis Factory.

[0021] Reagent:

[0022] Tetramethylbenzidine (TMB) was purchased from Shanghai Jingchun Industrial Co., Ltd.; other reagents were of analytical grade.

[0023] Proceed as follows:

[0024] 1. Preparation of immunogen:

[0025] Take 10 mg of mutant Salmonella typhimurium lipopolysaccharide Ra-LPS and dissolve it in 1 ...

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Abstract

The invention discloses a synthetic method of an immunogen for preparing a salmonella cross-type antiboby, and belongs to the technical field of immunoassay. The characteristic that mutant salmonella lipopolysaccharide (Ra-LPS) has no O-specificity side chain is utilized, the EDC and NHS method is adopted for coupling LPS tail end COOH and KLH, and mice sera obtained in an immunization mode have titer on the Ra-LPS and intact LPS. Salmonella thallus peridia of different serogroups are used for detecting the mice sera to obtain the result that all the mice sera have the cross reaction, and it is proved that the immunogen can be used for preparing the salmonella cross-type sera or monoclonal antibody. The synthetic method of the immunogen is used for preparing the salmonella cross-type antiboby, and therefore a solid foundation is laid for detecting the salmonella in the bacterial genus level.

Description

technical field [0001] The invention relates to a synthetic method for preparing an immunogen of Salmonella cross-type antibody, belonging to the technical field of immune analysis. Background technique [0002] Salmonella is a global foodborne pathogen. Biologically, Salmonella is a kind of Gram-negative bacteria with blunt ends, no spores, and generally no capsule. The main antigens are O antigen, H antigen, and Vi antigen. Animal foods such as poultry, eggs, and dairy products are easily contaminated with Salmonella. After the human body ingests food containing bacteria, it will cause acute gastroenteritis, typhoid fever, children with low immunity and even sepsis and other symptoms. [0003] There are more than 2,000 serotypes of Salmonella, and the common serotypes in clinical practice are mainly Salmonella enteritidis, Salmonella typhimurium, and Salmonella paratyphi A. The application of well-regulated production operations and management systems such as hazard ana...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K16/12C07K16/06
CPCC07K14/795C07K16/1235
Inventor 胥传来王文彬匡华徐丽广刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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