Gene chip for detecting six kinds of diarrhea pathogens and its prepn process and kit

A gene chip and pathogenic bacteria technology, applied in the field of bioengineering, can solve the problems of time-consuming and laborious, single detection method, etc., and achieve the effect of easy use
CN101045944AInactive Publication Date: 2007-10-03IPE BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
IPE BIOTECHNOLOGY CO LTD
Publication Date
2007-10-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to one kind of gene chip for detecting six kinds of diarrhea pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The oligonucleotide probe includes DNA or cDNA segments selected from nucleotide sequences corresponding to the genomes of Shigella, haemorrhagic colibacillus, invasive colibacillus, Vibrio parahaemolyticus, Vibrio cholreae and salmonella. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency and high detection accuracy.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering and the field of medical diagnosis, in particular to a gene chip for detecting six kinds of diarrhea pathogenic bacteria, a preparation method of the chip and a detection kit. Background technique

[0002] Six kinds of diarrhea pathogenic bacteria described in the present invention include:

[0003] Shigella flexneri (hereinafter abbreviated as SD)

[0004] Hemorrhagic Escherichia coli O157:H7 (Enterohemorrhagic E.coli O157:H7) (hereinafter abbreviated as EHEC)

[0005] Invasive Escherichia coli (Enteroinvasive E.coli) (hereinafter abbreviated as EIEC)

[0006] Vibrio parahaemolyticus (hereinafter abbreviated as VP)

[0007] Vibrio cholerae (Vibrio cholerae) (hereinafter abbreviated as VC)

[0008] Salmonella typhimurium (hereinafter abbreviated as SB)

[0009] Intestinal emerging pathogenic microbial infection is a serious hazard to human health, social stability and economic developm...

Claims

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