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Preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus

A swine flu virus and parvovirus technology, applied in the field of vaccines, can solve problems such as the difficulty of inoculating multiple pathogens at one time, and achieve the effect of improving immunity and production performance

Inactive Publication Date: 2017-12-22
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for preparing a triple inactivated vaccine of porcine erysipelas, porcine parvovirus, and swine influenza virus, so as to solve the problem that it is difficult for vaccine products in the prior art to simultaneously immunize the above-mentioned multiple vaccines at the same time. Pathogenic Technical Issues

Method used

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  • Preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus
  • Preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus
  • Preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus

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Experimental program
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Effect test

Embodiment 1

[0034] A preparation method of porcine erysipelas, porcine parvovirus and swine influenza virus triple inactivated vaccine, the steps are as follows:

[0035] 1 Production Seed Preparation

[0036] 1.1 Erysipelas suis production seed preparation

[0037] 1.1.1 Primary seed propagation

[0038] After unsealing the Erysipelas suis freeze-dried strains, add Martin broth to dilute and streak inoculate on the blood agar slope, culture at 37°C for 24 hours, streak on 10% serum Martin agar plate, culture for 24-36 hours, select a typical uniform Several bacterial colonies were inoculated on the slant of blood agar, cultured at 37°C for 24 hours, and used as primary seeds.

[0039] 1.1.2 Secondary seed propagation

[0040] Take the first-grade seeds and inoculate them in Martin Broth, incubate them at 37°C for 24 hours, then inoculate them in a large amount of meat liver and stomach digestion soup or Martin Broth, and incubate them for 24 hours, conduct a pure inspection according ...

Embodiment 3

[0083] Example 3 Vaccine Efficacy Research

[0084] 1 Experimental design

[0085] 1.1 Vaccine Immunization

[0086] Get the vaccine prepared in Example 1, mark the portion by the bottle label, 2 mL per head, intramuscularly inject 1 portion into 15 healthy susceptible pigs with a body weight of more than 20 kg 1-2 months after weaning, and 21 days after the first immunization. The dose and method of immunization were the same, and a control group of 15 animals was set up without immunization.

[0087]Purchase commercially available porcine erysipelas inactivated vaccine (C43-5), porcine parvovirus inactivated vaccine (CP-99), swine influenza virus H1N1 subtype inactivated vaccine, and inject the post-weaning 1 - 5 healthy susceptible pigs weighing more than 20kg for 2 months. The vaccine groups are shown in the table below.

[0088] Table 2 Vaccine immunization groups

[0089]

[0090]

[0091] 1.2 Antivirus

[0092] Three weeks after immunization, erysipelas, por...

Embodiment 4

[0101] The preparation method of porcine erysipelas, porcine parvovirus and swine influenza virus triple inactivated vaccine comprises the following steps:

[0102] 1) Cultivate the Erysipelas suis type 2 C43-5 strain to prepare a live bacterial liquid;

[0103] 2) Adjust the antigen concentration in the live Erysipelas suis type 2 C43-5 liquid obtained in step 1) to 10 by diluting or centrifuging. 9 CFU / mL;

[0104] 3) Utilizing formaldehyde to inactivate the Erysipelas suis type 2 C43-5 bacterium liquid after step 2) adjusting the concentration;

[0105] 4) Breeding porcine parvovirus BJ-2 strain to prepare live venom;

[0106] 5) using formaldehyde to inactivate the porcine parvovirus BJ-2 strain venom in step 4);

[0107] 6) Propagate swine influenza virus H1N1 strain to prepare live venom;

[0108] 7) using formaldehyde to inactivate the swine influenza virus H1N1 strain venom in step 6);

[0109] 8) The Erysipelas suis C43-5 bacterium liquid after step 3) inactivati...

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Abstract

The invention provides a preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus. The obtained vaccine immunization sow can achieve the effects of preventing various viruses by once injection; the blank of lack of the triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus in the market at present is filled. The method uses a specific immunologic adjuvant; after the vaccine immunization, the immunization position cannot generate red swelling and heat generation side reactions; the pig vitality and food intake conditions after the immunization cannot be influenced; the immunization pig immunization effects and the production performance are effectively improved. Compared with the single-antigen vaccine in the prior art, the triple inactivated vaccine has the advantages that the immunization efficiency is identical; the protection rate is higher than 80 percent. Meanwhile, the method provided by the invention has the advantages that the quality stability of the triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus can be effectively improved; the defects of operation complexity, great work intensity, easy pollution and the like in the spinner culture process are overcome.

Description

technical field [0001] The invention relates to the technical field of vaccines, and further relates to the preparation technology of multiple vaccines, in particular to a preparation method of a triple inactivated vaccine of porcine erysipelas, porcine parvovirus and swine influenza virus. Background technique [0002] Swine erysipelas (SE) is an acute, febrile infectious disease caused by Brysipelothrix rhusiopathiae, also known as red fever, also known as "fire mark". Clinically, high fever, rash and arthritis are the main features. Swine erysipelas is widely prevalent all over the world. It occurred frequently in my country in the 1980s. Together with swine fever and swine lung disease, it is called the three major infectious diseases of China's pig industry. In my country, swine erysipelas is listed as a second-class disease. The disease has been silent for more than 20 years. However, in recent years, swine erysipelas has occurred in Guangxi, Guangdong, Sichuan, Fujia...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/23A61K39/145A61K39/295A61P31/04A61P31/16A61P31/20
Inventor 厚华艳吕茂杰付旭彬郁宏伟梁武李守军
Owner TIANJIN RINGPU BIO TECH
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