Rapid test paper bar for testing colloidal gold of antibody of epidemic hemorrhagic fever virus

A technology for epidemic hemorrhagic fever and test strips, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem that the detection time cannot reach the rapid screening of patients, and achieve rapid screening of patients, easy promotion, and simple operation. Effect

Inactive Publication Date: 2007-05-16
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the traditional detection method of epidemic hemorrhagic fever virus antibody is serological diagnosis method, including the application of IgM capture ELISA to detect EHF IgM antibody, indirect enzyme immunosorbent assay to detect EHFIgG antibody, immunofluorescence test to detect double serum Ig

Method used

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  • Rapid test paper bar for testing colloidal gold of antibody of epidemic hemorrhagic fever virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Colloidal gold rapid detection test strip for antibody to epidemic hemorrhagic fever virus (see Figure 1)

[0030] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; Human IgM μ chain monoclonal antibody; containing 0.4cm×0.4cm colloidal gold-labeled epidemic hemorrhagic fever hantavirus recombinant antigen glass fiber membrane; the gold-labeled antigen protective film is a 2.7cm×0.4cm polyester film; Hemorrhagic fever virus antibody colloidal gold rapid detection test strip.

Embodiment 2

[0031] Example 2 Epidemic hemorrhagic fever virus antibody colloidal gold rapid detection test strip (see Figure 1)

[0032] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm filter paper; Anti-human IgMμ chain monoclonal antibody; containing 0.4cm×0.4cm colloidal gold-labeled recombinant antigen glass fiber membrane of epidemic hemorrhagic fever hantavirus; gold-labeled antigen protective film is 2.7cm×0.4cm glass fiber; that is, formed Colloidal gold rapid detection test strip for epidemic hemorrhagic fever virus antibody.

Embodiment 3

[0033] Example 3 Epidemic hemorrhagic fever virus antibody colloidal gold rapid detection test strip (see Figure 1)

[0034] The reaction support is 6.5cm×0.4cmm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; Human IgM μ chain monoclonal antibody; containing 0.4cm×0.4cm colloidal gold-labeled epidemic hemorrhagic fever Hantavirus recombinant antigen glass fiber membrane; the gold-labeled antigen protective film is filter paper fiber of 2.7cm×0.4cm; Hemorrhagic fever virus antibody colloidal gold rapid detection test strip.

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Abstract

This invention provides one test bar, which comprises the following steps: a, covering antibody IgM linkage single clone antibody and anti-flu hemorrhagic fever virus reset antigen multiple clone antibody two NC fiber films; b, comprising glue gold label flu hemorrhagic fever virus glass fiber film; applying film analysis and glue gold label technique to test sample flu virus antibody.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test strip for detecting antibodies to epidemic hemorrhagic fever virus and its application. Background technique [0002] Epidemic hemorrhagic fever (EHF) and epidemic nephropathy (NE) are collectively referred to internationally as hemorrhagic fever with renal syndrome (HFRS). (HFRS) is a type of natural foci disease caused by certain viruses in the Bunyaviridae genus Hantavirus and carried by certain rodents. EFH is prevalent in my country, and Apodemus spp. and Rattus norvegicus are its main hosts and sources of infection. Transmission is mainly through contact with host animals or their excreta (urine, feces) / secretion (saliva). my country is the country most seriously affected by hantavirus. In the past 10 years, 40,000 to 60,000 cases have been reported every year, accounting for more than 90% of the total number of cases reported in the world. EHF has a...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/544
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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