Mycoplasma pneumonia antigen, preparation method and immunodetection kit

Abstract

The invention provides a mycoplasma pneumonia (MP) antigen. The antigen fragment of the antigen is MP371 or MP661, wherein the locus of MP 371 is at an N terminal 371-480aa of MP adhesion protein P1, and the locus of MP661 is at an N terminal 661-772aa of MP adhesion protein P1. The invention also relates to a kit comprising any antigen or antigen composition and the application of the kit in detection of a mycoplasma pneumonia antibody. The mycoplasma pneumonia antigen is a recombinant antigen cloned and expressed by a codon-optimized gene and has stronger antigen specificity than that of the antigen cultivated and extracted by MP. The kit can be used for specific detection of an anti-MP-IgM (immunoglobulin m) antibody in clinical samples, thus identifying and diagnosing the infection of mycoplasma pneumonia at early stage.

Description

technical field [0001] The present invention relates to mycoplasma pneumoniae (MP) adhesion protein 1 (P1) codon optimized gene, its antigen, its antigen composition, and also relates to the kit and application containing the antigen or antigen composition. Background technique [0002] Mycoplasma pneumonia (MP) infection clinically causes atypical pneumonia and bronchitis, and severe cases can lead to complications other than the respiratory tract, such as acute diffuse encephalomyelitis, blood system, motor system damage, etc., and the infected objects are mostly Children under 5 years old. The symptoms of pneumonia caused by MP are similar to those caused by other pathogens, but MP has no cell wall structure, and the clinical medicine mainly uses macrolide antibiotics that can penetrate into cells, such as roxithromycin, erythromycin, azithromycin, etc. It is not sensitive to antibiotics used in conventional pneumonia treatment, such as penicillin and cephalosporins (mai...

AI Technical Summary

Problems solved by technology

[0006] Different from other pathogens, MP uses a unique biased codon system. The universal stop codon UGA encodes tryptophan in MP. If the wild gene of MP is used directly, protein translation can be interrupted, which limits the ability of recombinant MP antigens. Development

At present, the cloning and expression of recombinant MP antigens usually avoid UGA, and most of the obtained fragment antigens do not contain tryptophan, lack of effective prediction and screening of antigen epitopes, or directly use inactivated whole bacteria to extract antigens, which directly affects Development of Reagent for Detection of MP in Later Stage

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