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Dengue virus IgG/IgM antibody detection test strip, kit and preparation method thereof

A dengue virus and antibody detection technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problem of lack of dengue virus detection kits, etc., and achieve the effects of high detection sensitivity, small sample size, and simple operation

Inactive Publication Date: 2019-07-12
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is a lack of kits for dengue virus detection on the market.

Method used

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  • Dengue virus IgG/IgM antibody detection test strip, kit and preparation method thereof
  • Dengue virus IgG/IgM antibody detection test strip, kit and preparation method thereof
  • Dengue virus IgG/IgM antibody detection test strip, kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation method of the dengue virus IgG / IgM antibody detection card provided by the present embodiment, the specific steps are as follows:

[0056] The gold-spraying film-drawing instrument used in this example, model: XYZ3020, manufacturer: Shanghai Jiening Biotechnology Co., Ltd.;

[0057] Used in this example: dengue virus recombinant antigen, anti-human IgM antibody, anti-human IgG antibody, manufacturer: Feipeng Biological Co., Ltd.;

[0058] Used in this example: chicken IgY antigen, anti-chicken IgY polyclonal antibody, manufacturer: Hangzhou Qitai Biotechnology Co., Ltd.

[0059] (1) Preparation of colloidal gold solution

[0060] Taking 1L as an example, weigh 990g of water into an Erlenmeyer flask, add 10ml of 2% gold chloride solution, that is, the final concentration of gold chloride in the solution is 0.02%. Place the flask on a magnetic stirrer and heat it to boiling, then quickly add 13ml of 2% trisodium citrate solution, wait until the solution ...

Embodiment 2

[0099] Example 2 kit assembly

[0100] Take the aluminum foil bag and desiccant; open the heat sealer and preheat; put the test card and 1 bag of desiccant prepared in Example 1 to be bagged into the aluminum foil bag; seal the aluminum foil bag with a heat sealer; Label.

[0101] The aluminum foil bag with the detection card, the sampling straw, the sample diluent and the instruction box are the finished dengue virus IgG / IgM antibody detection kit.

Embodiment 3

[0103] Adopt the dengue virus IgG / IgM antibody detection card that embodiment 1 provides or the method for the dengue virus IgG / IgM antibody detection kit detection sample that embodiment 2 provides:

[0104] When using the dengue virus IgG / IgM antibody detection card to test, drop about 2 μl of serum / plasma into the sample well11, then drop 2-3 drops of sample diluent into the sample dilution well10, and place it horizontally. If the sample contains dengue virus IgM antibody, the dengue virus recombinant antigen-colloidal gold will combine with the dengue virus IgM antibody in the sample to form an "antibody-antigen-gold" complex, which is along the strip layer Analysis and movement, first reaching the detection line coated with anti-human IgM antibody, the "antibody-antigen-gold" complex will combine with anti-human IgM antibody, and stay on the T1 detection line to form a red line, which means IgM positive result. The shade of the line color is not proportional to the amou...

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Abstract

The invention discloses a dengue virus IgG / IgM antibody detection test strip, kit and preparation method thereof, and relates to the technical field of dengue virus detection. The dengue virus IgG / IgMantibody detection test strip of the invention comprises a sample pad, an immune combination pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad is sequentially stuck to the bottom of polyvinyl chloride; the immune combination pad is coated with dengue virus recombinant antigen marked by colloidal gold and chicken IgY polyclonal antibody; the nitrocellulose membrane is coated with dengue virus anti-human IgM antibody, a detection line of IgG antibody and a quality control line of anti-chicken IgY polyclonal antibody. The test strip can detect whether dengue virus IgG / IgM antibody exists in a sample to be detected through a method for detecting a marker. The test strip and the detection card comprising the test strip can be used as supplement for antigen detection onthe early acute infection of the dengue virus, covering the middle and later stages of the disease course and reducing the risk of missed detection.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of in vitro diagnosis, specifically relates to the technical field of biological detection, in particular to a preparation method of a dengue virus IgG / IgM antibody detection kit. [0003] Background technique: [0004] Dengue fever is an acute infectious disease caused by dengue virus. It is transmitted by Aedes aegypti and Aedes albopictus. Infectious diseases of most concern. Currently, it is endemic in more than 100 countries in Africa, the Americas, the Eastern Mediterranean, Southeast Asia and the Western Pacific, and Southeast Asia and the Western Pacific are more seriously affected by DV (dengue virus). About 250 million people live in dengue fever-endemic countries and can develop dengue fever, dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) after infection with DV. It is estimated that more than 50 million dengue infections occur each year, of which 500,000 people are hospita...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569
CPCG01N33/56983G01N33/6854Y02A50/30
Inventor 童国权杨标金巍刘中华王国强
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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