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Novel detection reagent card for coronavirus antibody detection and preparation method of detection reagent card

A coronavirus and antibody detection technology, applied in the field of quantum dot immunofluorescence chromatography in medical immunology, can solve the problems of cumbersome inspection and high inspection requirements, and achieve the effect of low detection cost, small size and reliable diagnostic results

Pending Publication Date: 2020-05-22
重庆新赛亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Detection of specific antibodies can clarify whether a patient has "recently or previously been infected with a new type of coronavirus", which can make up for the shortcomings of the clinical infection detection method of a new type of coronavirus (SARS-COV2), which is cumbersome and requires high requirements

Method used

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  • Novel detection reagent card for coronavirus antibody detection and preparation method of detection reagent card
  • Novel detection reagent card for coronavirus antibody detection and preparation method of detection reagent card
  • Novel detection reagent card for coronavirus antibody detection and preparation method of detection reagent card

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Experimental program
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Effect test

Embodiment

[0043] 1. Preparation of detection reagent card

[0044] 1. Optimization of sample pad 1:

[0045] 1.1. Materials and reagents:

[0046] (1) Sample pad 1: made of glass fiber.

[0047] (2) Sample pad optimization buffer: 50mmol / L, tris of pH8.2, Tween 20 with a concentration of 0.5% by mass, proclin300 with a concentration of 0.1% by mass, and The balance of purified water.

[0048] 1.2. Immerse the sample pad 1 in the sample pad optimization buffer solution for 30-45 minutes, then dry it in the drying room for more than 5 hours, and keep the humidity below 30%.

[0049] 2. Optimization of Quantum Mat 2:

[0050] 2.1. Materials and reagents

[0051] (1) Quantum pad 2: glass fiber material.

[0052] (2) Quantum pad optimization buffer: 50mmol / L, tris of pH8.2, bovine serum albumin (BSA) with a mass percentage concentration of 1%, and a mass percentage concentration of 0.5% Tween 20, 2% sucrose by mass, 0.1% proclin300 by mass, and purified water as the balance.

[0053]...

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Abstract

The invention relates to a novel detection reagent card for coronavirus antibody detection. The reagent card comprises a sample pad, a quantum pad, a nitrocellulose membrane, absorbent paper and a lining plate, the quantum pad is coated with a quantum dot microsphere labeled anti-human IgM antibody or IgG antibody, a detection line arranged on the nitrocellulose membrane is coated with an SARS-COV2 recombinant antigen, and a quality control line is coated with goat anti-mouse IgG. According to the detection reagent card for detecting the novel coronavirus (SARS-COV2) IgM antibody or IgG antibody provided by the invention, a result can be rapidly and accurately obtained, and auxiliary diagnosis is provided for clinical COVID-19.

Description

technical field [0001] The invention belongs to the technical field of quantum dot immunofluorescence chromatography in medical immunology, and in particular relates to a detection reagent card for novel coronavirus antibody detection and a preparation method thereof. Background technique [0002] Studies have shown that: Coronavirus (Coronaviruses) is a single-stranded positive-strand RNA virus, which is divided into four genera: α, β, γ and δ: the new coronavirus (SARS-COV2) is a new type of coronavirus, belonging to the genus β, Classified as the seventh type of coronavirus. On January 12, 2020, it was named a new type of coronavirus (SARS-COV2) by the World Health Organization, which can cause viral pneumonia. [0003] "Pneumonia Diagnosis and Treatment Plan for Novel Coronavirus Infection (Trial Version 5)" stated that its incubation period is 1-14 days, generally 3-7 days. The main manifestations are fever, fatigue, and dry cough. A small number of patients are acco...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/533
CPCG01N33/56983G01N33/558G01N33/54313G01N33/533G01N2333/165
Inventor 张念林何乐春胡昌华左云国孙婵
Owner 重庆新赛亚生物科技有限公司
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