Application of mycobacterium tuberculosis protein in preparation of products used for diagnosis of latent tuberculosis infection
A pulmonary tuberculosis and latent technology, applied in the field of biomedicine, can solve the problems that latent tuberculosis infection cannot be diagnosed and the positive detection rate is low.
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Embodiment 1
[0072] Embodiment 1, the preparation of protein chip
[0073] (1) Preparation of Mycobacterium tuberculosis antigenic proteins Rv2693c and Rv1984c
[0074] 1. Express
[0075] The genetically engineered Saccharomyces cerevisiae uses galactose to induce overexpression, and the specific process is as follows:
[0076] First, from Mycobacterium M. tuberculosis H37Rv strain (Beijing strain), the gene coding fragments of protein Rv2693c and Rv1984c were de novo cloned by PCR, and the fragments were connected to the pDONR221 vector ( purchased from Invitrogen), transformed into Escherichia coli DH5-Alpha and amplified, the extracted vector was then replaced by LR enzyme (Invitrogen) to the modified pEGH-A vector capable of expressing GST tags (the vector is in "Jian Zhu, Heng Zhu , et al: J.Virol.May2009vol.83no.105219-5231", the public can obtain from Guangdong Tibikang Biotechnology Co., Ltd.), and then transformed into E. coli DH5-Alpha to amplify, and extract the plasmid for t...
Embodiment 2
[0135] Example 2. Application of protein chip for auxiliary diagnosis of latent pulmonary tuberculosis infection
[0136] (1) Preparation of serum samples to be tested
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