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Treponema pallidum antibody test kit and preparation method and detection method thereof

A Treponema pallidum and kit technology, applied in the field of immunoassay medical diagnosis, can solve the problems that the results are easily affected by subjective judgment, high cost, unsuitable for batch specimen detection, etc.

Inactive Publication Date: 2010-11-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TPPA method is a confirmatory test with high specificity, but the cost is high, the operation is cumbersome, the results are easily affected by subjective judgments, and it is not suitable for batch specimen testing

Method used

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  • Treponema pallidum antibody test kit and preparation method and detection method thereof
  • Treponema pallidum antibody test kit and preparation method and detection method thereof

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Embodiment Construction

[0022] 1. A kind of component embodiment of the Treponema pallidum antibody detection kit based on the flow microsphere carrier method involved in the present invention:

[0023] (1) Polymer sphere microcarrier coated with genetically engineered recombinant TP antigen

[0024] (2) Fluorescence-labeled anti-human immunoglobulin antibody

[0025] (3) Negative control serum

[0026] (4) Positive control serum

[0027] (5) Sample diluent

[0028] 2. A kind of preparation embodiment based on flow microsphere carrier method Treponema pallidum (TP) antibody detection kit involved in the present invention:

[0029] (1) Polymer sphere microcarrier coated with genetically engineered recombinant TP antigen:

[0030] The material of the microsphere carrier is latex, silicon, resin, styrene, bromostyrene, or polyurethane made of acrylic, acrylic amine, methacrylate, chlorinated vinyl, chlorinated styrene, ethylene acetate and polymerizable monomers. Coat polystyrene microspheres with...

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Abstract

The invention relates to a treponema pallidum (TP) antibody test kit based on the flow microspheric carrier technology and a preparation method and detection method thereof, belonging to the technical field of immunoassay medical diagnosis. The preparation method comprises the following steps: using TP recombinant antigen to coat heavy polymer microsphere, using bovine serum albumin to seal empty binding site, preparing specific TP probe-heavy polymer microsphere; performing coculture with a sample to be tested to capture TP antibody, washing and centrifuging to remove the unbound TP antibody, then adding fluorescently-labeled anti-human IgG or IgM antibody; and using a flow cytometry to detect the fluorescence intensity of the microsphere, and performing qualitative or quantitative analysis to the tested antibody. The method has the advantages of high sensitivity and specificity and good stability and can be used to perform microanalysis or multivalent analysis to the sample.

Description

technical field [0001] The invention relates to a Treponema pallidum (TP) antibody detection kit based on flow microsphere carrier technology and a preparation and detection method thereof, belonging to the technical field of immunoanalysis medical diagnosis. Background technique [0002] The incidence of syphilis in my country has been increasing year by year in recent years. In April 2010, the Ministry of Health announced that among the 27 types of legally reported Class A and Class B infectious diseases in the country, the top five diseases were viral hepatitis, tuberculosis, dysentery, syphilis and influenza A (H1N1), among which syphilis Ranked third, fourth in 2009, and fifth in 2005. [0003] Syphilis is a chronic systemic infectious disease not limited to the skin or reproductive system that occurs after the human body is infected with Treponema pallidum, and is mainly transmitted through sexual contact, mother to child, and blood. At present, the detection of seru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N33/532
Inventor 林远王月华
Owner NANJING UNIV OF TECH
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