Method for detecting Lyme disease pathogen in tick bodies and kit

Abstract

The invention relates to a loop-mediated isotheral amplification method (LAMP) for detecting Lyme disease pathogen in tick bodies, which is characterized by taking 16S rRNA gene as the target gene, using special software to design the LAMP for detecting the primers of the Lyme disease pathogen-Borrelia burgdorferi in the tick bodies, then selecting the primer of the specific fragment of the Lyme disease pathogen from the primers, extracting DNA after piercing the ticks to be detected, uniformly mixing the obtained DNA and the reaction buffer, the reaction mixture of the selected primer of the specific fragment and DNA polymerase to carry out amplification, adding loading buffer after activating the amplified product, then placing the mixture into agarose gel containing ethidium bromide to carry out electrophoresis detection and detecting whether the detected ticks carry the Lyme disease pathogen according to whether the specific band occurs after electrophoresis.

Description

technical field [0001] The invention relates to a pathogen detection technology, in particular to a LAMP detection method for detecting Lyme disease pathogen in ticks. Background technique [0002] Lyme disease, also known as Lyme soft body disease or Lyme borreliosis, is a tick-borne natural foci of zoonotic spirochetes, which was first diagnosed in Lyme Township, Connecticut, USA. Discovered and named. The pathogen is Borrelia burgdorferi, which can be divided into 10 genotypes or groups. The reported Lyme disease spirochetes in my country include Borrelia burgdorferi sensus stricto, B. Three genotypes of B. afzelii. At present, it has been reported in more than 30 countries and regions in the world that the disease and natural foci exist. The annual incidence is about 300,000, and there is still an increasing trend. It is called "the second AIDS" in the United States (Gratz N. Emerging and resurging vector-borne disease. Ann Rev Entomol, 1999, 44:51). In 1992, the Worl...

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Problems solved by technology

[0006] The present invention aims at the shortcomings of complex instruments and low sensitivity when detecting Lyme disease pathogen Borrelia burgdorferi in ticks in the prior art, and provides a molecular detection method with high sensitivity that only needs a conventional water bath. The detection sensitivity of this method is higher than that of the PCR method, and at the same time, it does not require a complicated operating system, and can be detected under ordinary laboratory conditions, and has the characteristics of simple operation, high sensitivity, and rapidity.

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