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Test paper bar for testing colloidal gold of antibody of dengue fever virus

A technique for detecting dengue virus and test strips, which is applied in the field of dengue virus antibody detection test strips, can solve the problems of cumbersome, time-consuming, and complicated operations, and achieve the effects of simple operation, clear and easy-to-distinguish results, and simple methods

Inactive Publication Date: 2007-05-16
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of the prior art that the operation is complex, cumbersome, time-consuming, requires special instruments, and requires professionals to operate

Method used

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  • Test paper bar for testing colloidal gold of antibody of dengue fever virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Dengue virus antibody colloidal gold detection test strip (see Figure 1)

[0033] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; a 2.5cm×0.4cm nitrocellulose membrane is coated with anti-human IgM, dengue virus recombinant antigen and anti-recombinant antigen polyclonal antibody in turn Containing 0.4cm * 0.4cm colloidal gold-labeled dengue virus-specific recombinant antigen glass fiber membrane; the gold-labeled antigen protective film is a polyester film of 2cm * 0.4cm; promptly formed a dengue virus antibody colloidal gold detection test strip.

Embodiment 2

[0034] Embodiment 2: Dengue virus antibody colloidal gold detection test strip (see Figure 1)

[0035] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 2.5cm×0.4cm nitrocellulose membrane is coated with anti-human IgM, dengue virus recombinant antigen and anti-recombinant antigen polyclonal antibody in turn Contain the glass fiber membrane of the dengue fever virus-specific recombinant antigen of 0.4cm * 0.4cmm colloidal gold label; The gold label antigen protection film is the glass fiber of 2cm * 0.4cm; Promptly formed the dengue fever virus antibody colloidal gold detection test strip.

Embodiment 3

[0036] Embodiment 3: Dengue virus antibody colloidal gold detection test strip (see Figure 1)

[0037] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 2.5cm×0.4cm nitrocellulose membrane is coated with anti-human IgM, dengue virus recombinant antigen and anti-recombinant antigen polyclonal antibody in turn Contain the glass fiber membrane of the dengue fever virus-specific recombinant antigen of 0.4cm * 0.4cmm colloidal gold label; The filter paper fiber that the gold label antigen protection film is 2cm * 0.4cm; Promptly formed the dengue fever virus antibody colloidal gold detection test strip.

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Abstract

This invention provides one glue gold test bar to test dengue fever virus IgG and IgM antibody, which separately covers IgM antibody and dengue virus antigen and antigen multiple clone antibody on NC film and combines glue gold label dengue virus reestablish antigen and applies immune film analysis technique and tests dengue virus IgG and IgM antibody.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a dengue virus antibody detection test strip and an application thereof. Background technique [0002] Dengue fever (Dengue Fever, referred to as DF) is an acute infectious disease caused by dengue virus. Dengue virus belongs to Flavivirus (Flaviviridae), belongs to group B of arbovirus according to epidemiological classification, and is divided into 4 serotypes serologically, mainly through Aedes aegypti and Aedes albopictus (commonly known as flower Mosquitoes) spread. Dengue fever is widely prevalent in tropical and subtropical regions of the world. It is the most widely distributed, most frequently occurring, and harmful arbovirus disease. [0003] Commonly used antibody detection methods are: ELISA method and RT-PCR method. However, these detection techniques require special instruments and equipment, and require professional technicians to perform detectio...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/544
CPCY02A50/30
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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