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Application of suite reagent for detecting EV71-VP1 antibody of hand-foot-mouth disease patient in disease outcome prediction

A hand-foot-mouth and antibody technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficult treatment timing, lack of reference indicators, etc., and achieve the effect of reducing the occurrence of deaths.

Inactive Publication Date: 2011-01-05
INST OF BASIC MEDICINE OF SAMS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few omens for the occurrence and development of severe diseases, and there is a lack of clinical reference indicators, so it is very difficult to control the timing of treatment clinically.

Method used

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  • Application of suite reagent for detecting EV71-VP1 antibody of hand-foot-mouth disease patient in disease outcome prediction
  • Application of suite reagent for detecting EV71-VP1 antibody of hand-foot-mouth disease patient in disease outcome prediction

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Assembling a complete set of reagents, and using it to detect the patient's antibody titer

[0020] 1 Preparation of a set of reagents

[0021] (1) Preparation of ELISA plates: mouse anti-human IgG and IgM were used as capture antibodies, and 1×10 -5 , 1×10 -6 After dilution (the diluent is carbonate buffer solution at pH 9.6), they were respectively coated with polystyrene plates, sealed and washed by known methods, and stored at 4°C.

[0022] (2) Preparation of detection reagent: use genetic engineering method to clone and express EV71 VP1, and use known method to purify, horseradish peroxidase (HRP) label, titrate working concentration (10 -5 ~10 -7 ).

[0023] 2 EV71 patient serum detection and outcome prediction, using the above kit of reagents for detection

[0024] (1) Infer the development trend of the disease by detecting the serum antibody titer of the patient: dilute the coagulated serum from the veins of children A, B, and C at 1:2, 1:4, and 1...

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Abstract

The invention discloses a suite reagent for titrating the titer of an EV71IgM antibody and an EV71IgG antibody of a hand-foot-mouth disease patient, comprising an ELISA (Enzyme Linked Immuno Sorbent Assay) plate and a detection reagent. The ELISA plate of the EV71IgM antibody comprises a polystyrene board, 1gM capture antibody is coated in polystyrene board holes, the ELISA plate of the EV71IgG antibody comprises a polystyrene board, and 1gM capture antibody is coated in the polystyrene board holes, and the detection reagent is EV71VP1 labeled by HRP (Horseradish Peroxidase). The suite reagent of the invention can be used for detecting the antibody titer of the hand-foot-mouth disease patient, the detection result can be used as a judgment result for judging disease outcome so that a clinical severe case can be predicted, prevented and controlled to lessen the probability of death cases.

Description

technical field [0001] The invention relates to a set of reagents for titrating EV71IgM antibody and EV71IgG antibody titer of hand-foot-mouth disease patients and its application in predicting disease outcome of hand-foot-mouth disease patients. Background technique [0002] The pathogen of severe HFMD is mainly enterovirus serotype 71 (EV71), and its main clinical manifestations are nervous system damage, accompanied by / or heart, lung damage or even functional failure. Clinically, the development of the disease is often unpredictable, and children who are still in good condition can undergo rapid changes in a short period of time, and even die suddenly. Timely passive immunotherapy (such as immunoglobulin, convalescent serum, etc.) for critically ill patients can turn the crisis into safety. However, there are few omens for the occurrence and development of severe diseases, and there is a lack of clinical reference indicators, so it is very difficult to control the timing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/545
Inventor 孟红盖中涛岳盈盈李鹏宋楠楠李志会张颖
Owner INST OF BASIC MEDICINE OF SAMS
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