Composition, kit and method used for detecting enterovirus causing hand foot and mouth disease
A technology for enterovirus and hand, foot and mouth disease, applied in biochemical equipment and methods, recombinant DNA technology, microbe measurement/inspection, etc., to save manpower and time, detect products without opening the cover, and improve detection efficiency
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Embodiment 1
[0082] A composition for detecting hand, foot and mouth disease-causing enterovirus, consisting of the following primers:
[0083] P1: 5'-CCCTGAATGCGGCTAATCC-3',
[0084] P2: 5'-GTCGGTTCCGCTGCAGAGT-3'.
Embodiment 2
[0086] A composition for detecting hand, foot and mouth disease-causing enterovirus, consisting of the following primers and probes:
[0087] P1: 5'-CCCTGAATGCGGCTAATCC-3',
[0088] P2: 5'-GTCGGTTCCGCTGCAGAGT-3';
[0089] Probe1: 5′-fluorophore-AACTGCGGAGCACACGCCCAC-fluorescent quencher-3′,
[0090] Probe2: 5'-fluorescent group-CTGCGGAGCGCGYACCCTCA-fluorescent quencher-3', where Y=T or C.
Embodiment 3
[0092] A composition for detecting hand, foot and mouth disease-causing enterovirus, consisting of the following primers:
[0093] P1: 5'-CCCTGAATGCGGCTAATCC-3',
[0094] P2: 5'-GTCGGTTCCGCTGCAGAGT-3';
[0095] P3: 5'-CAGCCCAAAAGAACTTCACTATGAA-3',
[0096] P4: 5'-TCAATTACATCTGCCACCCTATCTC-3';
[0097] P5: 5'-ATTGGTGCTCCCACTACAGC-3',
[0098] P6: 5'-CAGTGTTGGCAGCTGTAGGTAGTAC-3'.
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