Water extract of houttuynia cordata thunb and preparation method and application of water extract

A technology for water extract and Houttuynia cordata, which is applied in the field of Houttuynia cordata water extract and its preparation, and achieves the effect of simple extraction process

Active Publication Date: 2013-05-01
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are no effective vaccines and drugs against HFMD virus clinically, and the situation

Method used

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  • Water extract of houttuynia cordata thunb and preparation method and application of water extract
  • Water extract of houttuynia cordata thunb and preparation method and application of water extract
  • Water extract of houttuynia cordata thunb and preparation method and application of water extract

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1 Extraction and detection of Houttuynia cordata water extract and polysaccharide

[0023] (1) Extraction of Houttuynia cordata water extract and polysaccharide

[0024] Firstly, the dried plant of Houttuynia cordata is washed, dried at low temperature to constant weight, and divided into two parts, one part is used for extracting water extract, and the other part is used for extracting Houttuynia cordata polysaccharide. Add deionized water according to the plant mass and liquid volume ratio of 1:10 (g / ml), keep warm in a water bath at 60°C to 90°C for 2-5 hours, centrifuge at 1000g / min for 10min to obtain the supernatant water extract, and precipitate the filter residue by the same method After leaching twice according to the method, the filtrates were combined, the supernatant was centrifuged at 5000g / min for 10min, and filtered through a 0.45μm filter membrane. The supernatant liquid is directly freeze-dried to obtain the freeze-dried powder of the water ext...

Embodiment 2

[0027] Example 2 Detection of Houttuynia cordata water extract and polysaccharide anti-hand, foot and mouth disease virus activity

[0028] (1) Toxicity test of Houttuynia cordata water extract and Houttuynia cordata polysaccharide on cells

[0029] Cultivate Vero cells in a 96-well plate. After the cells grow into a monolayer, reconstitute the lyophilized powder of the water extract and the alcohol-precipitated polysaccharide obtained above with deionized water, and use final concentrations of 1 μg / ml and 3 μg / ml respectively. , 10 μg / ml, 30 μg / ml, 100 μg / ml, 300 μg / ml, and 1000 μg / ml samples were used to treat Vero cells, and three replicate wells were set up. At the same time, a normal control group without drug treatment was set up. At 37°C, 5% CO 2 Cultivate in an incubator to observe the toxicity of the sample to Vero cells. After culturing for 72 hours, the classic MTT method for detecting cell viability is used to measure the toxicity of the sample to Vero cells. Sp...

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Abstract

The invention belongs to the technical field of biological pharmacy, and particularly relates to a water extract of houttuynia cordata thunb and a preparation method and an application of the water extract. Total polysaccharide obtained from the water extract of the houttuynia cordata thunb has activity to inactivate or inhibit replication of hand-foot-mouth disease viruses, namely an enterovirus-71 (EV-71) and coxsackie A-16 (CVA16); polysaccharide with the antiviral activity is further found to be polysaccharide with molecular weight greater than 100kDa; therefore, the polysaccharide can be applied in preparation of a medicine for preventing the hand-foot-and-mouth disease viruses. As an edible aquatic herbaceous plant, the houttuynia cordata thunb has the characteristics of extensive source, small toxic and side effects, low price, simple extraction process and the like, and has important development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a water extract of Houttuynia cordata and its preparation method and application. Background technique [0002] Houttuynia cordata Thunb is a perennial herbaceous plant belonging to the genus Houttuynia cordata. Widely distributed in the central, southeast and southwest provinces of my country. Houttuynia cordata has high nutritional value and is one of the commonly used wild vegetables. At the same time, it has the effects of clearing heat and detoxification, eliminating carbuncle and discharging pus, diuresis and treating stranguria, and belongs to the plant resources that are both medicine and food determined by the Ministry of Health. [0003] The chemical components of Houttuynia cordata mainly include volatile oil, alkaloids, flavonoids, organic acids, polysaccharides and other components. The main components of the volatile oil are decanoyl acetaldehyde (hout...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61K31/715A61P31/14C08B37/00
Inventor 李尔广陈晓庆王伟金玉
Owner NANJING UNIV
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