Homogeneous immunodetection kit for detecting target immunoglobulin M (IgM) antibody in sample as well as using method and application of homogeneous immunodetection kit

Abstract

The invention relates to a homogeneous immunodetection kit for detecting a target immunoglobulin M (IgM) antibody in a sample as well as a using method and application of the homogeneous immunodetection kit. The kit comprises a reagent a, a reagent b and a reagent c, wherein the reagent a comprises a known antigen, and the known antigen can be specifically bonded to the target IgM antibody in thesample to be detected so as to form a first complex; the reagent b comprises a complement C1q, the complement C1q can be specifically bonded to the first complex so as to form a second complex, and the complement C1q is not enabled to be bonded to a free IgM antibody; any of the known antigen and the complement C1q is connected with a receptor; the receptor can react with received singlet oxygen so as to produce detectable chemiluminescence signals; the reagent c comprises a donor, and the donor can generate the singlet oxygen in an excited state. A method for detecting the target IgM antibodyin the sample by using the kit overcomes the effect of a non-specific IgM antibody on detection.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a homogeneous immunoassay kit for detecting target IgM antibodies in a sample and its use method and application. Background technique [0002] The detection of IgM antibodies is of great significance in medical testing. When a pathogen first infects the body, the first antibody produced is always IgM antibody. With the progress of the body's immune response, antibody class switching occurs to produce IgG, IgM or IgA antibody. Therefore, in the diagnosis of infectious diseases, a positive IgM antibody test is a sign of current infection. [0003] At present, the commonly used IgM antibody detection method is the capture method, which is to coat the anti-human IgM (μ chain) antibody on the solid phase carrier, and after the serum / plasma sample reacts with the solid phase carrier, all the IgM antibody in the sample Captured on the solid phase carrier, aft...

AI Technical Summary

Problems solved by technology

This method has the following two disadvantages: one is that the anti-human IgM antibody is used as a capture antibody and cannot distinguish between specific IgM antibodies and non-specific IgM antibodies. When the concentration of specific IgM antibodies is low and the concentration of non-specific IgM antibodies is particularly high, it may Presence of specific IgM antibodies is removed by washing, resulting in false negative results

The second is that the detection process requires two steps of incubation and washing. In the washing process, it is very dependent on the washing efficiency of the washing equipment, which is prone to problems such as poor repeatability. The two incubation steps are cumbersome and time-consuming, which reduces work efficiency.

However, this method adds a new biologically active substance, and there are too many protein components in the reaction system, which may cause false positives

In addition, non-specific IgM antibodies in the homogeneous reaction system will also consume a large amount of anti-human IgM antibodies. When the concentration of specific IgM antibodies is low and the concentration of non-specific IgM antibodies is particularly high, it may also cause false negatives.

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