Preparation method and applications of biotin marked rabbit anti-tilapia IgM (immunoglobulin m) polyclonal antibody

A polyclonal antibody and biotin-labeled technology, which is applied in biological testing, material inspection products, measuring devices, etc., to achieve the effects of high titer, good specificity, important theoretical significance and production value
CN103454411AActive Publication Date: 2013-12-18INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
Publication Date
2013-12-18

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Abstract

The invention discloses a preparation method of a biotin marked rabbit anti-tilapia IgM (immunoglobulin m) polyclonal antibody. The preparation method comprises the following steps: collecting tilapia serum, and purifying to obtain purified tilapia IgM; immunizing a rabbit by using the tilapia IgM to obtain a rabbit anti-tilapia IgM polyclonal antibody, and detecting the titer of anti-tilapia serum of the rabbit anti-tilapia IgM polyclonal antibody by adopting ELISA (enzyme linked immunosorbent assay); strengthening the immunity of the purified tilapia IgM, then collecting and separating to obtain serum containing the rabbit anti-tilapia IgM polyclonal antibody, purifying, adding biotin and mixing uniformly to obtain the biotin marked rabbit anti-tilapia IgM polyclonal antibody. The biotin marked rabbit anti-tilapia IgM polyclonal antibody prepared by the method disclosed by the invention is good in specificity and high in purity and titer, and can have a specific binding reaction with the tilapia IgM. The invention also discloses applications of the biotin marked rabbit anti-tilapia IgM polyclonal antibody prepared by the method in ELISA and Western-Blot.
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Description

technical field

[0001] The invention belongs to the technical field of polyclonal antibodies, in particular to a preparation method and application of a biotin-labeled rabbit anti-tilapia IgM polyclonal antibody. Background technique

[0002] Immunoglobulin is an important molecule in the humoral immune response of animals, and the main immunoglobulin of teleosts as lower vertebrates is IgM. The molecule is a tetramer containing 2 heavy chains and 2 light chains per monomer. Highly purified fish serum IgM has important uses in fish immunology, such as preparing monoclonal and polyclonal antibodies, detecting fish pathogens and fish immune response levels; exploring the production and distribution of IgM, and can also be used to study immune System evolution.

[0003] Tilapia has been listed as a high-quality fish species for industrial development by the Ministry of Agriculture. In recent years, with the destruction of the breeding environment and the increase of breeding ...

Claims

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