Immune globulin M detection reagent
A technology for immunoglobulin and detection reagents, which is applied in the field of reagents for determining serum components, can solve the problems of inability to analyze and detect batch samples, cumbersome operations, etc., and achieve the effects of high accuracy, good repeatability, and strong anti-interference ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] a. An IgM reactant (or sample diluent) that fully exposes the IgM antigenic site in the sample and is conducive to fully combining with the anti-IgM antibody reagent, including 0.01%-1% preservative, 0.05% -1% stabilizer, 0.1-10% electrolyte, 2%-8% polymer accelerator, 0.1-10% surfactant and 0.1%-0.5% reaction accelerator (preservative, stabilizer, electrolyte, high The content ratio of molecular accelerator, surfactant and reaction accelerator is the weight percent with IgM reactant), also includes 5-200mmol / L buffer solution;
[0028] As the buffer, it can be phosphate buffer, tris buffer, PIPES buffer, HEPES buffer, TAPS buffer, glycine buffer, boric acid buffer, etc., the present invention preferably phosphate buffer, buffer The pH value is 5-10, preferably 7.4-8.1.
[0029] Can be nonionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant as surfactant, preferred nonionic surfactant here, have as example: Theist, Tween series, polyoxye...
Embodiment 1
[0050] 1. IgM reagent (R1)
[0051] Phosphate buffer 100mmol / L
[0052] Polyoxyethylene lauryl ether (surfactant) 5%
[0053] NaCl (electrolyte) 10%
[0054] PEG-6000 (polymer accelerator) 4%
[0055] Broad-spectrum fungicide (preservative) 0.1%
[0056] Hexadimethylamine bromide (reaction accelerator) 0.3%
[0057] Disodium EDTA (stabilizer) 0.05%
[0058] 2. Anti-IgM antibody reagent (R2)
[0059] Phosphate buffer 100mmol / L
[0060] Polyoxyethylene lauryl ether (surfactant) 5%
[0061] NaCl (electrolyte) 10%
[0062] PEG-6000 (polymer accelerator) 4%
[0063] Broad-spectrum fungicide (preservative) 0.4%
[0064] Disodium EDTA (stabilizer) 0.05%
[0065] Rabbit anti-human IgM antiserum 10%
[0066] Butylated hydroxyanisole (antioxidant) 0.01%
[0067] Mannitol (stabilizer) 5%
[0068] 3. Liquid serotype IgM constant value calibration solution
[0069] Buffer CAPSO 100mmol / L
[0070] Preservative Broad-spectrum fungicide 0.01%
[0071] Stabilizer Disodium edet...
Embodiment 2
[0077] 1. IgM reagent (R1)
[0078] Phosphate buffer 5mmol / L
[0079] Polyoxyethylene lauryl ether (surfactant) 0.1%
[0080] NaCl (electrolyte) 0.1%
[0081] PEG-6000 (polymer accelerator) 7%
[0082] Broad-spectrum fungicide (preservative) 0.01%
[0083] Polybrene (reaction accelerator) 0.1%
[0084] Disodium EDTA (stabilizer) 1.0%
[0085] 2. Anti-IgM antibody reagent (R2)
[0086] Phosphate buffer 5mmol / L
[0087] Polyoxyethylene lauryl ether (surfactant) 0.1%
[0088] NaCl (electrolyte) 0.1%
[0089] PEG-6000 (polymer accelerator) 7%
[0090] Broad-spectrum fungicide (preservative) 0.01%
[0091] Disodium EDTA (stabilizer) 2%
[0092] Goat anti-human IgM antiserum 45%
[0093] Butylated hydroxyanisole (antioxidant) 0.5%
[0094] Magnesium chloride (stabilizer) 1%
[0095] 3. Liquid serotype constant value IgM calibration solution
[0096] Buffer CAPSO 20mmol / L
[0097] Preservative Broad-spectrum fungicide 1%
[0098] Stabilizer Glycerol 1%
[0099] Stab...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com