Immune globulin M detection reagent

A technology for immunoglobulin and detection reagents, which is applied in the field of reagents for determining serum components, can solve the problems of inability to analyze and detect batch samples, cumbersome operations, etc., and achieve the effects of high accuracy, good repeatability, and strong anti-interference ability

Inactive Publication Date: 2009-02-18
王贤理
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Known methods for measuring immunoglobulin M (IgM) include immunodiffusion method, immunoelectrophoresis method, and radioimmunoassay method. These methods are cumbersome to operate, require special equipment, and require pretreatment of samples. Batch sample analysis and analysis cannot be performed. Disadvantages such as not being able to be directly tested by a fully automatic biochemical analyzer

Method used

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  • Immune globulin M detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] a. An IgM reactant (or sample diluent) that fully exposes the IgM antigenic site in the sample and is conducive to fully combining with the anti-IgM antibody reagent, including 0.01%-1% preservative, 0.05% -1% stabilizer, 0.1-10% electrolyte, 2%-8% polymer accelerator, 0.1-10% surfactant and 0.1%-0.5% reaction accelerator (preservative, stabilizer, electrolyte, high The content ratio of molecular accelerator, surfactant and reaction accelerator is the weight percent with IgM reactant), also includes 5-200mmol / L buffer solution;

[0028] As the buffer, it can be phosphate buffer, tris buffer, PIPES buffer, HEPES buffer, TAPS buffer, glycine buffer, boric acid buffer, etc., the present invention preferably phosphate buffer, buffer The pH value is 5-10, preferably 7.4-8.1.

[0029] Can be nonionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant as surfactant, preferred nonionic surfactant here, have as example: Theist, Tween series, polyoxye...

Embodiment 1

[0050] 1. IgM reagent (R1)

[0051] Phosphate buffer 100mmol / L

[0052] Polyoxyethylene lauryl ether (surfactant) 5%

[0053] NaCl (electrolyte) 10%

[0054] PEG-6000 (polymer accelerator) 4%

[0055] Broad-spectrum fungicide (preservative) 0.1%

[0056] Hexadimethylamine bromide (reaction accelerator) 0.3%

[0057] Disodium EDTA (stabilizer) 0.05%

[0058] 2. Anti-IgM antibody reagent (R2)

[0059] Phosphate buffer 100mmol / L

[0060] Polyoxyethylene lauryl ether (surfactant) 5%

[0061] NaCl (electrolyte) 10%

[0062] PEG-6000 (polymer accelerator) 4%

[0063] Broad-spectrum fungicide (preservative) 0.4%

[0064] Disodium EDTA (stabilizer) 0.05%

[0065] Rabbit anti-human IgM antiserum 10%

[0066] Butylated hydroxyanisole (antioxidant) 0.01%

[0067] Mannitol (stabilizer) 5%

[0068] 3. Liquid serotype IgM constant value calibration solution

[0069] Buffer CAPSO 100mmol / L

[0070] Preservative Broad-spectrum fungicide 0.01%

[0071] Stabilizer Disodium edet...

Embodiment 2

[0077] 1. IgM reagent (R1)

[0078] Phosphate buffer 5mmol / L

[0079] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0080] NaCl (electrolyte) 0.1%

[0081] PEG-6000 (polymer accelerator) 7%

[0082] Broad-spectrum fungicide (preservative) 0.01%

[0083] Polybrene (reaction accelerator) 0.1%

[0084] Disodium EDTA (stabilizer) 1.0%

[0085] 2. Anti-IgM antibody reagent (R2)

[0086] Phosphate buffer 5mmol / L

[0087] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0088] NaCl (electrolyte) 0.1%

[0089] PEG-6000 (polymer accelerator) 7%

[0090] Broad-spectrum fungicide (preservative) 0.01%

[0091] Disodium EDTA (stabilizer) 2%

[0092] Goat anti-human IgM antiserum 45%

[0093] Butylated hydroxyanisole (antioxidant) 0.5%

[0094] Magnesium chloride (stabilizer) 1%

[0095] 3. Liquid serotype constant value IgM calibration solution

[0096] Buffer CAPSO 20mmol / L

[0097] Preservative Broad-spectrum fungicide 1%

[0098] Stabilizer Glycerol 1%

[0099] Stab...

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Abstract

The invention discloses an immunoglobulin M detection reagent, which is provided with simple operation, high accuracy and good reproducibility and strong anti-interference ability; the sample is free from dilution and the detection reagent can be applicable to the immunoglobulin M (IgM) detection reagents of various automatic biochemical analyzers. The immunoglobulin M detection reagent comprises an IgA reagent, an anti-IgA antibody reagent and a liquid serotype constant value calibration liquid; wherein, the IgA reagent enables the IgA antigenic sites in the sample to be fully exposed so as to facilitate the full combination with the anti-IgA antibody reagent; the anti-IgA antibody reagent has high idiosyncrasy with the IgA antigens in human serum; and the liquid serotype constant value calibration liquid is compared with the sample for result calculation.

Description

technical field [0001] The invention relates to a reagent for measuring serum components, in particular to a reagent for measuring immunoglobulin M (IgM) in serum, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] Immunoglobulins are a group of proteins with antibody activity, mainly found in plasma, but also in other body fluids, tissues and some secretions. Most of the immunoglobulins in human plasma exist in gamma globulin (γ-globulin). Can be divided into five classes, namely immunoglobulin G (IgG), immunoglobulin A (IgM), immunoglobulin M (IgM), immunoglobulin D (IgD) and immunoglobulin E (IgE). Immunoglobulin M (IgM) is the most molecular weight of the five classes of immunoglobulins. Its molecular weight is 900,000 Daltons, so it is also called macroglobulin. The average concentration in normal human serum is 60-180 mg%, accounting for about 6% of the total serum immunoglobulin. It mainly exists in blood v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 王贤理蒙凯蔡其浩
Owner 王贤理
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