HSV-I and HSV-II IgM antibody joint inspection kit and preparation method thereof

A herpes simplex virus and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation process, inconvenient and fast detection, etc., and achieve the effect of simplified operation process, rapid response and simple operation

Inactive Publication Date: 2011-06-29
北京库尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although separate detection of herpes simplex virus type I and type II IgM and comprehensive analysis of the test results have no effect on the diagnosis of the disease, the operation process is cumbersome and the detection is not convenient and quick enough

Method used

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  • HSV-I and HSV-II IgM antibody joint inspection kit and preparation method thereof
  • HSV-I and HSV-II IgM antibody joint inspection kit and preparation method thereof
  • HSV-I and HSV-II IgM antibody joint inspection kit and preparation method thereof

Examples

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Embodiment 1

[0034] Example 1: Preparation of Herpes Simplex Virus Type I, Type II IgM Antibody Joint Detection Kit

[0035] 1. Preparation of mouse anti-human IgM monoclonal antibody

[0036] Using monoclonal antibody preparation method, hybridoma cell lines were routinely cultured and passaged in vitro, injected with human IgM into the peritoneal cavity of BALB / C mice, collected ascites, and purified mouse anti-human IgM monoclonal antibody by affinity chromatography.

[0037] 2. Preparation of Herpes Simplex Virus Type I Antigen and Herpes Simplex Virus Type II Antigen

[0038] Culture human embryonic diploid cells to a single layer, inoculate the standard strain of herpes simplex virus type I, wait for 2-3 days after the inoculation of the type I strain of herpes simplex virus, 100% of the cells are pathological, collect the culture medium and / pathological cells, PBS Wash 3 times and add glycine-sodium hydroxide buffer, pH 9.0. After freezing and thawing for 3 times, sonicate, take t...

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Abstract

The invention relates to a herpes simplex virus I and II (HSV-I and HSV-II) immunoglobulin M (IgM) antibody joint inspection kit and a preparation method thereof. The kit comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample absorption pad (4) and a bottom plate (5), wherein the colloidal gold pad is a colloidal gold-labeled mouse anti-human IgM monoclonal antibody glass fiber or a non-woven fabric; and the nitrocellulose membrane is coated with an HSV-I antigen and an HSV-II antigen serving as detection lines, and a goat anti-mouse IgM antibody serving as a quality control line respectively. HSV-I and HSV-II IgM antibodies are detected by a colloidal gold immunochromatography technological principle and can be jointly detected through one-time operation, so that the operation process is simplified, and the kit has the characteristics of quick response, high sensitivity and the like, is easy to operate and is economical and practical.

Description

technical field [0001] The invention belongs to the technical field of biological applications, in particular to a herpes simplex virus type I and type II IgM antibody joint detection kit and a preparation method thereof. Background technique [0002] Herpes simplex virus (herpes simplex virus) belongs to the herpesviridae A virus subfamily, and the size of the virus plasmid is about 180nm. According to the difference in antigenicity, human herpes simplex virus is currently divided into two types, namely, herpes simplex virus type I (HSV-I) and herpes simplex virus type II (HSV-II). Type I herpes disease is mainly transmitted through close contact with the respiratory tract, skin and mucous membranes, and infects the skin mucous membranes and organs above the waist. Such as causing inflammation and herpes of the mucous membranes of the lips, nasal vestibule, conjunctiva, throat, and herpes around the mouth and mouth, 99% are caused by type I herpes virus infection. Type II...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571
Inventor 李峰陈立柱杨利
Owner 北京库尔科技有限公司
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