Colloidal gold test strip for fast detecting IgG and IgM antibodies of dengue fever virus

A technology of dengue virus and colloidal gold test paper, which is applied in the field of biological detection, can solve the problems of cumbersome detection process, unsuitable detection, complicated operation, etc., and achieve the effect of improving reaction rate, short reaction time and simple operation

Inactive Publication Date: 2012-10-03
蓝十字生物药业(北京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection techniques require special instruments and equipment, and require professional technicians to perform detection in a professional laboratory. The operation is complicated and time-consuming, and it is not suitable for on-site detection.
[0008] At present, there is no instrument in the prior art that can realize the simultaneous detection of dengue virus IgG and IgM antibodies with serotypes 1, 2, 3, and 4 and has the characteristics of simplicity, speed, and accuracy. Therefore, for the detection of dengue virus IgG and IgM antibodies Detection takes a long time and the detection process is cumbersome

Method used

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  • Colloidal gold test strip for fast detecting IgG and IgM antibodies of dengue fever virus
  • Colloidal gold test strip for fast detecting IgG and IgM antibodies of dengue fever virus
  • Colloidal gold test strip for fast detecting IgG and IgM antibodies of dengue fever virus

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0051] Experimental example 1. the preparation method of test strip of the present invention

[0052] Reagents: four antigens of four subtypes of dengue virus and polyclonal antibodies against dengue virus antigens were purchased from the Institute of Microbial Epidemiology, Academy of Military Medical Sciences of the Chinese People's Liberation Army; anti-μ chain monoclonal antibodies and anti-human IgG monoclonal antibodies were purchased from Imported from Artron BioResearch Inc., Canada.

[0053] The test strip preparation procedure is as follows

[0054] Coating of cellulose film

[0055] Step 1. Use anti-dengue virus antigen as control line C, anti-μ chain monoclonal antibody as detection line T1, and anti-human IgG monoclonal antibody as detection line T2. ), diluted with 20mM PBS.

[0056] Step 2. Coat the prepared above three reagents on the nitrocellulose membrane at positions C, T1 and T2 respectively, and then dry for 6 hours at a temperature of 30°C and a humidit...

Embodiment 2

[0070] Embodiment 2. Using method and methodological comparative experiment

[0071] How to use this product:

[0072] Take 1 μl of the sample to be tested, drop it on the sample fiber layer below the MAX line of the liquid end of the horizontal test strip, and then drop 2 drops (80-100 μl) of the sample diluent on the sample fiber layer.

[0073] Comparison 1: Product Comparison

[0074] The product of the present invention has the characteristics of rapidity, accuracy, specificity and convenience compared with the products of the prior art for rapid detection of dengue virus IgG and IgM antibodies, and can qualitatively detect four dengue viruses of serotypes 1, 2, 3 and 4 IgG, IgM antibodies, before and after the rapid detection of dengue virus IgG, IgM antibodies do not need to do the necessary instrument cleaning and maintenance work, and does not require professionals in the field to operate.

[0075] Control product 1: "Dengue virus IgM antibody diagnostic kit (enzyme...

Embodiment 3

[0099] Compared with a colloidal gold test strip for detecting dengue virus IgG and IgM antibodies disclosed in the patent application number 200510086853.2, the present invention has the following advantages:

[0100] 1. Sample size. Compared with the invention patent application number 200510086853.2, which discloses a colloidal gold detection test strip for detecting dengue virus IgG and IgM antibodies, the consumption is much less, saving resources and cost.

[0101] The comparison data are as follows:

[0102] project

Comparison product

Product of the present invention

sample size

150-200μl

1μl

[0103] 2. The present invention can be prepared by colloidal gold, and made into a single type of colloidal gold reagent or a mixed colloidal gold reagent of type 1-4, which can distinguish the serotype of the test sample, and the application number is 200510086853.2 invention patent publication A product of colloidal gold test strips for ...

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Abstract

The invention discloses a colloidal gold test strip for fast detecting IgG (immunoglobulin G) and IgM (immunoglobulin M) antibodies of dengue fever virus and belongs to medical test consumables. The colloidal gold test strip is characterized in that 1-4 kinds of antigens are enveloped on a gold labeled protein-adsorbed fiber combination pad; the antigens are selected from four subtype antigens of the dengue fever virus labeld by colloidal gold; a polyclonal antibody of the antigens for resisting the dengue fever virus is enveloped on a quality control line C; an anti-mu chain monoclonal antibody is enveloped on a detection line T1, and an anti-human IgG monoclonal antibody is enveloped on a detection line T2. The test strip disclosed by the invention can fast detect serotype 1, 2, 3 and 4 type IgG and IgM antibodies of the dengue fever virus by adopting an antibody capture method combined with an immune colloidal gold technique and has the advantages of simplicity, sensitivity, specificity and accuracy. The test strip disclosed by the invention has the advantages of small sample quantity and wide storage condition, can save resources and can judge the positive result of the IgG or IgM antibody of the dengue fever virus once at the same time.

Description

[0001] technology field, [0002] The invention belongs to the field of biological detection and relates to a medical detection consumable, in particular to a colloidal gold test strip for rapid detection of dengue virus IgG and IgM antibodies. Background technique [0003] Dengue fever is an acute infectious disease caused by dengue virus and transmitted by Aedes mosquitoes. The clinical features are sudden onset, high fever, muscle, bone marrow and arthralgia all over the body, extreme fatigue, and some patients have rash, bleeding tendency and enlarged lymph nodes. Dengue fever is the most serious mosquito-borne viral disease in the world. The virus is mainly transmitted by the Aedes aegypti mosquito. More than 100 countries have reported dengue fever outbreaks. There are 250 million people living in dengue high-infection areas. There will be 50 million to 100 million cases of dengue hemorrhagic fever every year, and 200,000-500,000 cases of dengue fever. Infected individ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCY02A50/30
Inventor 张莹秦成峰孙茜
Owner 蓝十字生物药业(北京)有限公司
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