Eucheuma compound for improving lung function and preparation method thereof

Abstract

The invention provides an eucheuma compound for improving lung function and a preparation method thereof. The eucheuma compound is prepared from the following raw materials in parts by weight: 25 to 35 parts of eucheuma extract, 13 to 17 parts of oyster peptide, 8 to 12 parts of fish collagen peptide, 4 to 6 parts of rhizoma polygonati, 3 to 5 parts of herba cistanche, 2 to 4 parts of semen euryales and 1 to 2 parts of longan. Secondly, the eucheuma extract rich in eucheuma polysaccharide, eucheuma polypeptide and the like is prepared through the technology. The components in the formula of the eucheuma compound have a synergistic effect, and the prepared eucheuma compound can effectively control the lung coefficient and increase of the hydroxyproline content in lung tissue and improve inflammatory infiltration and fibrohyperplasia of the lung. In addition, the compound also inhibits oxidative stress reaction and further inhibits pulmonary fibrosis; the lung lesion is effectively inhibited, the lung function is improved, and the lung health is promoted.

Description

Technical Field

[0001] The invention relates to the field of deep processing of plant extracts, and in particular to an Eucheuma complex for improving lung function and a preparation method thereof. Background Art

[0002] With the development of modern society and changes in lifestyle, lung health issues are receiving increasing attention. Factors such as air pollution, smoking, and occupational exposure have led to a significant increase in the incidence of lung-related diseases, especially chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, and asthma. These diseases not only seriously affect the quality of life of patients, but also bring huge economic burdens to society and the medical system. Existing drug treatments usually focus on relieving symptoms, but it is difficult to fundamentally improve lung function or restore damaged lung tissue.

[0003] In recent years, more and more studies have begun to focus on the potential of natural plant ingredients in promoting lung health. As a plant rich in multiple bioactive ingredients, Eucheuma has been shown to have multiple biological functions such as anti-oxidation, anti-inflammation, improving microcirculation and promoting cell regeneration. The flowers and fruits of Eucheuma are rich in nutrients such as flavonoids and organic acids.

[0004] Although studies have shown that Eucheuma extract has a certain positive effect on lung health, there is a relative lack of products that improve lung function, and consumers often find it difficult to find efficient and safe options. Therefore, a formulated Eucheuma complex was developed to achieve better results in improving lung function and promoting lung health. Summary of the invention

[0005] In view of this, the present invention proposes a Eucheuma complex for improving lung function and a preparation method thereof to solve the above problems.

[0006] The technical solution of the present invention is achieved as follows: a Eucheuma complex for improving lung function is prepared by including the following raw materials in parts by weight: 25-35 parts of Eucheuma extract, 13-17 parts of oyster peptide, 8-12 parts of fish collagen peptide, 4-6 parts of Polygonatum sibiricum, 3-5 parts of Cistanche deserticola, 2-4 parts of Euryale ferox, and 1-2 parts of longan.

[0007] The method for preparing the Eucheuma complex for improving lung function of the present invention comprises the following steps:

[0008] (1) taking polygonatum, cistanche deserticola, euryale ferox and longan, adding water at a material-liquid ratio of 1:14-16 kg / L and boiling, and collecting the decoction;

[0009] (2) Add the Eucheuma extract to the decoction, and finally add the oyster peptide and fish collagen peptide, homogenize, and sterilize.

[0010] A further technical solution is that it also contains Citrus aurantium and Ophiopogon japonicus, which is made from the following raw materials in parts by weight: 25-35 parts of Eurycoma longifolia extract, 13-17 parts of oyster peptide, 8-12 parts of fish collagen peptide, 2-4 parts of Polygonatum sibiricum, 3-5 parts of Cistanche deserticola, 4-6 parts of Euryale ferox, 1-2 parts of Longan, 0.9-1.2 parts of Citrus aurantium and 0.5-0.8 parts of Ophiopogon japonicus.

[0011] A further technical solution is that the Eucheuma complex is made from the following raw materials in parts by weight: 30 parts of Eucheuma extract, 15 parts of oyster peptide, 10 parts of fish collagen peptide, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 3.0 parts of Euryale ferox, 1.0 part of Longan, 1.0 part of Citrus aurantium, and 0.7 part of Ophiopogon japonicus.

[0012] A further technical solution is that the preparation method of the Eucheuma extract comprises the following steps:

[0013] S1. Take Eucheuma, wash it with water, dry it, soak it in a sodium chloride solution, and add a composite enzyme for enzymolysis, wherein the composite enzyme consists of cellulase, papain and pullulanase;

[0014] S2. After enzymatic hydrolysis, water is added to perform water extraction, the extract is collected, filtered, the filtrate is collected, alcohol precipitation is performed, suction filtration is performed, and drying is performed to obtain an Eucheuma extract.

[0015] A further technical solution is that in step S1, the complex enzyme is composed of cellulase, papain and pullulanase in a mass ratio of 6-8:3-5:0.8-1.2.

[0016] A further technical solution is that in step S1, the mass concentration of the sodium chloride solution is 10% to 30%, and the soaking time is 20 to 40 minutes.

[0017] A further technical solution is that in step S2, the amount of water added is 20 to 30 times that of Eucheuma.

[0018] A further technical solution is that in step S2, the extraction temperature is 85-90°C and the extraction time is 1-2h.

[0019] The present invention provides a method for preparing a Eucheuma complex for improving lung function, comprising the following steps:

[0020] (1) taking polygonatum, cistanche deserticola, euryale ferox and longan, adding water at a material-liquid ratio of 1:14-16 kg / L and boiling, and collecting the decoction;

[0021] (2) Add the Eucheuma extract to the decoction, and finally add the oyster peptide and fish collagen peptide, homogenize, and sterilize.

[0022] The present invention also provides another method for preparing a Eucheuma complex for improving lung function, comprising the following steps:

[0023] (1) Take Ophiopogon japonicus and Citrus aurantium, add 95% v / v ethanol solution according to the solid-liquid ratio of 1:8-12 kg / L, reflux extraction for 2-4 hours, repeat the extraction 2-3 times, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain a composite extract;

[0024] (2) taking Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, adding water at a material-liquid ratio of 1:14-16 kg / L, and boiling, and collecting the decoction;

[0025] (3) Add the composite extract and the Eucheuma extract to the decoction, and finally add the oyster peptide and the fish collagen peptide, homogenize, and sterilize.

[0026] A further technical solution is that in step (1), each reflux extraction time is 2-4 hours.

[0027] A further technical solution is that in step (2), the decoction time is 1-2 hours.

[0028] A further technical solution is that in step (3), the homogenization pressure is 25-35 MPa and the homogenization time is 5-10 min.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention uses Eucheuma extract as the main raw material, preferably oyster peptide, fish collagen peptide, Polygonatum, Cistanche deserticola, Euryale ferox, and Longan, and prepares them according to a certain ratio to achieve synergistic effect. The prepared Eucheuma complex can effectively control the increase of lung coefficient and hydroxyproline content in lung tissue, improve inflammatory infiltration and fibrosis in the lung. In addition, the complex also inhibits oxidative stress response and further inhibits pulmonary fibrosis; it not only effectively inhibits lung lesions, improves lung function, and promotes lung health.

[0031] (2) Adding Citrus aurantium and Radix Ophiopogonis to the formula of the present invention can further enhance and improve lung function, help reduce lung coefficient and hydroxyproline content in lung tissue, help dredge lung qi, moisturize the lungs, improve the body's immune function, and enhance the self-repair ability of the lungs.

[0032] (3) The Eucheuma extract prepared by the process of the present invention fully softens its cell wall, and is enzymatically hydrolyzed by using a certain proportion of cellulase, papain and pullulanase to fully release more active ingredients and improve the extraction efficiency. The prepared Eucheuma extract is rich in Eucheuma polysaccharides, Eucheuma polypeptides, etc., and effectively improves the bioavailability.

[0033] (4) The Eucheuma composite liquid prepared by the present invention is rich in various nutrients and helps to improve immune function. DETAILED DESCRIPTION

[0034] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0035] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0036] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0037] The Eucheuma used in the present invention is red algae Eucheuma gelatinae.

[0038] Preparation Example 1

[0039] The preparation method of Eucheuma extract comprises the following steps: taking red algae Eucheuma agarica, washing with water, drying, soaking in a 20%wt sodium chloride solution for 30 minutes, adding a composite enzyme for enzymolysis, wherein the composite enzyme is composed of neutral cellulase, papain and pullulanase in a mass ratio of 7:4:1, adding water after enzymolysis, the solid-liquid ratio of Eucheuma to water is 1:25 kg / L, the extraction temperature is 87°C±2°C, the extraction time is 1.5h, collecting the extract, filtering, collecting the filtrate, precipitating with alcohol, filtering by suction, and drying (removing ethanol) to obtain the Eucheuma extract.

[0040] The following examples all use the Eucheuma extract of Preparation Example 1.

[0041] Example 1

[0042] (I) A Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of the Eucheuma extract of Preparation Example 1, 15 parts of oyster peptide, 10 parts of fish collagen peptide, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 4.0 parts of Euryale ferox, and 2.0 parts of Longan.

[0043] (ii) A method for preparing the Eucheuma complex, comprising the following steps:

[0044] (1) Take Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, add water at a material-liquid ratio of 1:15 kg / L, and boil for 1.5 h, and collect the decoction;

[0045] (2) The Eucheuma extract was added to the decoction, and finally oyster peptide and fish collagen peptide were added, homogenized at 30 MPa for 8 min, and sterilized to obtain the Eucheuma composite solution.

[0046] The nutritional component test results of Eucheuma compound liquid (specification 100 ml / bottle) are shown in Table 1.

[0047] Testing unit: Guangzhou Jinzhi Testing Technology Co., Ltd.

[0048] Table 1 Nutritional composition

[0049]

[0050] The Eucheuma composite liquid prepared by the invention contains various nutrients such as protein, dietary fiber, calcium, magnesium and the like.

[0051] Example 2

[0052] On the basis of Example 1, add Citrus aurantium and Radix Ophiopogonis, and reduce the amount of Euryale ferox and Longan added.

[0053] (I) A Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of the Eucheuma extract of Preparation Example 1, 15 parts of oyster peptides, 10 parts of fish collagen peptides, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 3.0 parts of Euryale ferox, 1.0 part of Longan, 1.0 part of Citrus aurantium, and 0.7 part of Ophiopogon japonicus.

[0054] (ii) A method for preparing the Eucheuma complex, comprising the following steps:

[0055] (1) Take Ophiopogon japonicus and Citrus aurantium, add 95% v / v ethanol solution according to the solid-liquid ratio of kg / L of 1:10, reflux extraction at 60°C±5°C for 1.5h, repeat the extraction twice, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain a composite extract;

[0056] (2) Take Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, add water at a material-liquid ratio of 1:15 kg / L, and boil for 1.5 h, and collect the decoction;

[0057] (3) The composite extract and the Eucheuma extract were added to the decoction, and finally the oyster peptide and the fish collagen peptide were added. The mixture was homogenized at 30 MPa for 8 min and sterilized to obtain the Eucheuma composite solution.

[0058] Example 3

[0059] (I) A Eucheuma complex, comprising the following raw materials in parts by weight: 28 parts of the Eucheuma extract of Preparation Example 1, 16 parts of oyster peptides, 12 parts of fish collagen peptides, 4 parts of Polygonatum sibiricum, 5 parts of Cistanche deserticola, 2 parts of Euryale ferox, 1.3 parts of Longan, 0.9 parts of Ophiopogon japonicus, and 0.8 parts of Citrus aurantium.

[0060] (ii) A method for preparing the Eucheuma complex, comprising the following steps:

[0061] (1) Take Ophiopogon japonicus and Citrus aurantium, add 95% v / v ethanol solution according to the solid-liquid ratio of kg / L of 1:10, reflux extraction at 60°C±5°C for 1.5h, repeat the extraction twice, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain a composite extract;

[0062] (2) Take Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, add water at a material-liquid ratio of 1:15 kg / L, and boil for 1.5 h, and collect the decoction;

[0063] (3) The composite extract and the Eucheuma extract were added to the decoction, and finally the oyster peptide and the fish collagen peptide were added. The mixture was homogenized at 30 MPa for 8 min and sterilized to obtain the Eucheuma composite solution.

[0064] Example 4

[0065] (I) A Eucheuma complex, comprising the following raw materials in parts by weight: 32 parts of the Eucheuma extract of Preparation Example 1, 13 parts of oyster peptides, 9 parts of fish collagen peptides, 6 parts of Polygonatum sibiricum, 3 parts of Cistanche deserticola, 4 parts of Euryale ferox, 1.3 parts of Longan, 1.2 parts of Ophiopogon japonicus, and 0.5 parts of Citrus aurantium.

[0066] (ii) A method for preparing the Eucheuma complex, comprising the following steps:

[0067] (1) Take Ophiopogon japonicus and Citrus aurantium, add 95% v / v ethanol solution according to the solid-liquid ratio of kg / L of 1:10, reflux extraction at 60°C±5°C for 1.5h, repeat the extraction twice, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain a composite extract;

[0068] (2) Take Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, add water at a material-liquid ratio of 1:15 kg / L, and boil for 1.5 h, and collect the decoction;

[0069] (3) The composite extract and the Eucheuma extract were added to the decoction, and finally the oyster peptide and the fish collagen peptide were added. The mixture was homogenized at 30 MPa for 8 min and sterilized to obtain the Eucheuma composite solution.

[0070] Comparative Example 1

[0071] (I) Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of Spirulina extract, 15 parts of oyster peptide, 10 parts of fish collagen peptide, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 4.0 parts of Euryale ferox, and 2.0 parts of Longan. The main difference from Example 1 is that Eucheuma extract is not added to the formula, but the amount of Spirulina extract (food grade, commercially available) is increased by an equal amount;

[0072] (II) The preparation method of the Eucheuma complex is the same as that in Example 1.

[0073] Comparative Example 2

[0074] (I) Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of Eucheuma extract of Preparation Example 1, 25 parts of fish collagen peptide, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 4.0 parts of Euryale ferox, and 2.0 parts of Longan. The main difference from Example 1 is that oyster peptide is not added to the formula, but the amount of fish collagen peptide added is increased by an equal amount;

[0075] (II) The preparation method of the Eucheuma complex is the same as that in Example 1.

[0076] Comparative Example 3

[0077] (I) Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of Eucheuma extract of Preparation Example 1, 25 parts of oyster peptide, 5.0 parts of Polygonatum sibiricum, 4.0 parts of Cistanche deserticola, 4.0 parts of Euryale ferox, and 2.0 parts of Longan. The main difference from Example 1 is that fish collagen peptide is not added to the formula, but the amount of oyster peptide added is increased by the same amount;

[0078] (II) The preparation method of the Eucheuma complex is the same as that in Example 1.

[0079] Comparative Example 4

[0080] The main difference from Example 1 is the preparation of the Eucheuma extract:

[0081] (1) Eucheuma complex, comprising the following raw materials in parts by weight: 30 parts of Eucheuma extract, 15 parts of oyster peptide, 10 parts of fish collagen peptide, 5.0 parts of Polygonatum, 4.0 parts of Cistanche deserticola, 4.0 parts of Euryale ferox, and 2.0 parts of Longan. The preparation method of the above-mentioned Eucheuma extract comprises the following steps: taking Eucheuma, washing it with water, drying it, soaking it in a 20%wt sodium chloride solution for 30 minutes, adding a composite enzyme for enzymolysis, wherein the composite enzyme is composed of cellulase and papain in a mass ratio of 7:5, adding water after enzymolysis, the solid-liquid ratio of Eucheuma to water is 1:25 kg / L, the extraction temperature is 87°C±2°C, the extraction time is 1.5h, collecting the extract, filtering, collecting the filtrate, precipitating with alcohol, filtering by suction, and drying (removing ethanol) to obtain the Eucheuma extract. The main difference from Preparation Example 1 is that pullulanase is not added in the enzymolysis, but the amount of papain added is increased by an equal amount;

[0082] (II) The preparation method of the Eucheuma complex is the same as that in Example 1.

[0083] Test Example 1-Toxicity Test

[0084] 1 Experimental method: 30 SPF KM mice were selected and randomly divided into 3 groups according to gender and weight, namely, Eucheuma complex groups 1-2 (corresponding to Example 1 and Example 2, respectively) and a vehicle control group, with 10 mice in each group, half of which were male and half were female. The mice in Eucheuma complex groups 1-2 were intragastrically administered with the test substance twice within 24 hours, with an interval of about 4 hours between the two administrations, a total dose of 5000 mg / kg for the two administrations, and a volume of 40 mL / kg for each administration. The vehicle control group was administered with the same volume of 0.5% CMC-Na solution.

[0085] After administration, animals were observed clinically in general, including appearance, behavior, response to stimulation, secretions and excretions. Symptoms after administration of the test substance, the onset of symptoms, death and time of death were recorded. Close observation was performed within 3 hours after administration. The animals were observed twice a day (on the 1st and afternoon) for the first 3 days after administration, and then once a day for 14 days. The animals were weighed and their food intake was measured on D1, D4, D8, D1, and D15 of administration. All animals were euthanized with CO2 after the experiment, and gross dissection was performed. Changes in organs and tissues were observed with the naked eye and recorded. Histopathological examination was performed when changes in volume, color, texture, etc. were found in tissues and organs.

[0086] 2Test results:

[0087] 2.1 General observations: After administration of the Eucheuma complex, the mice were observed for 14 consecutive days. The behavioral activities and mental state of the mice were normal. No animal deaths or abnormal clinical manifestations were observed in Eucheuma complex groups 1-2 and the vehicle control group.

[0088] 2.2 Body weight: After 14 days of continuous observation after administration of the Eucheuma complex, the body weight of the two groups of animals showed an overall increasing trend. Compared with the vehicle control group, there were no significant differences in body weight change and weight gain in Eucheuma complex groups 1-2 on D1, 4, 8, 11, and 15 (P>0.05).

[0089] 2.3 Food intake: From D1 to D4 after administration of the Eucheuma complex, the average daily food intake of each animal in the Eucheuma complex group 1-2 was significantly lower than that in the vehicle control group. This may be because the test substance (Echeuma complex) can provide a certain amount of energy requirements, and the mice feel full after oral administration, so the average daily food intake is reduced. From D8 to D15, the average daily food intake of the Eucheuma complex group 1-2 was slightly lower than that in the vehicle control group, and the difference between the two groups did not exceed 0.5g / day. During this period, there was no significant difference in the body weight of the two groups of mice, which was considered to be a fluctuation within the normal food intake range and had nothing to do with the effect of the test substance.

[0090] 2.4 Gross anatomical examination: All animals in Eucheuma complex groups 1-2 and vehicle control group were euthanized as planned at the end of the experiment. Macroscopic observation of the dissection showed that their body surfaces were intact, their fur was smooth, there were no external injuries or ulcers, no subcutaneous spots or pigmentation, and no obvious lesions or abnormalities were found in the volume, color and texture of various organs and tissues.

[0091] 3 Conclusion: Under the experimental conditions, the Eucheuma complex prepared in Example 1-2 showed no toxic reaction to KM mice.

[0092] Test Example 2-Efficacy Test

[0093] Bleomycin-induced pulmonary fibrosis mice were used as a model. 110 SPF C57 mice, weighing 20-25g, female were selected. After one week of adaptive feeding, they were randomly divided into a blank control group, a model group, a test group 1-8, and a positive control group (pirfenidone), with 10 mice in each group. In addition to the blank control group, 0.30%wt sodium pentobarbital was anesthetized by intraperitoneal injection at a dose of 50mg / kg, and then the mice were fixed on the operating table in a supine position. Use tweezers to gently pull out the tongue to expose the trachea, and with the assistance of a cold light source magnifying glass, slowly inject 5.00mg / kg of bleomycin solution. After the injection is completed, the mouse plate is quickly erected and rotated for 3 minutes to ensure that the solution is evenly distributed in the lungs. The blank control group also uses the above method to inject an equal volume of 0.9% sodium chloride solution. The day of atomization was recorded as the first day, and each group of mice was gavaged with different reagents. Test groups 1-8 were given the Eucheuma complex prepared by Examples 1-4 and Comparative Examples 1-4, respectively, at a daily dose of 5g / kg. The blank control group and the model group were given the same amount of normal saline. The positive control group was given pirfenidone at a daily dose of 300 mg / kg.

[0094] Mouse samples were collected on the 28th day. Sample collection method: After killing the mice, they were dissected along the midline of the abdomen, the lungs were removed, the lungs were rinsed with saline, the surface moisture was absorbed and then weighed. The upper sample of the left lung was placed in a 4% paraformaldehyde solution for fixation, and the lower part of the left lung was stored in a -80°C refrigerator for later use. The remaining lung tissue and pre-cooled PBS buffer were placed in a glass homogenizer at a ratio of mass (g) to volume (mL) of 1:9, and manually ground up and down for 5 minutes. Centrifuge at 6000rpm and 4°C for 10 minutes, and remove the upper layer of lung tissue homogenate clear liquid. The clear liquid will be used for subsequent detection of hydroxyproline (HYP).

[0095] The lung coefficient was calculated, and the hydroxyproline content in lung tissue was determined using a hydroxyproline kit.

[0096] Lung coefficient = (lung tissue weight / mouse body weight) * 100%

[0097] The results are shown in Table 2 below.

[0098] Table 2 Results of determination of mouse lung coefficient and lung tissue hydroxyproline content

[0099]

[0100]

[0101] The above results show that for mice with bleomycin-induced pulmonary fibrosis, the Eucheuma complex prepared in Examples 1-4 can not only effectively control the increase of the lung coefficient, but also significantly inhibit the increase of the hydroxyproline content in the lung tissue, effectively inhibit lung lesions, and effectively improve lung function, among which the Eucheuma complex prepared in Example 2 has a better effect.

[0102] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A Eucheuma complex for improving lung function, characterized in that: The invention comprises the following raw materials in parts by weight: 25-35 parts of Eucheuma extract, 13-17 parts of oyster peptide, 8-12 parts of fish collagen peptide, 4-6 parts of Polygonatum sibiricum, 3-5 parts of Cistanche deserticola, 2-4 parts of Euryale ferox and 1-2 parts of Longan.

2. The Eucheuma complex for improving lung function according to claim 1, characterized in that The invention also contains tangerine peel and ophiopogon japonicus, and is made from the following raw materials in parts by weight: 25-35 parts of Eucheuma extract, 13-17 parts of oyster peptide, 8-12 parts of fish collagen peptide, 2-4 parts of polygonatum, 3-5 parts of cistanche, 4-6 parts of euryale, 1-2 parts of longan, 0.9-1.2 parts of tangerine peel and 0.5-0.8 parts of ophiopogon japonicus.

3. The Eucheuma complex for improving lung function according to claim 2, characterized in that The invention is prepared by the following raw materials in parts by weight: 30 parts of Eucheuma extract, 15 parts of oyster peptide, 10 parts of fish collagen peptide, 5 parts of Polygonatum sibiricum, 4 parts of Cistanche deserticola, 3 parts of Euryale ferox, 1 part of Longan, 1 part of Citrus aurantium, and 0.7 parts of Ophiopogon japonicus.

4. The Eucheuma complex for improving lung function according to any one of claims 1 to 3, characterized in that The preparation method of the Eucheuma extract comprises the following steps: S1. Take Eucheuma, wash it with water, dry it, soak it in a sodium chloride solution, and add a composite enzyme for enzymolysis, wherein the composite enzyme consists of cellulase, papain and pullulanase; S2. After enzymatic hydrolysis, water is added to perform water extraction, the extract is collected, filtered, the filtrate is collected, alcohol precipitation is performed, suction filtration is performed, and drying is performed to obtain an Eucheuma extract.

5. The Eucheuma complex for improving lung function according to claim 4, characterized in that In step S1, the complex enzyme is composed of cellulase, papain and pullulanase in a mass ratio of 6-8:3-5:0.8-1.

2.

6. The Eucheuma complex for improving lung function according to claim 4, characterized in that In step S1, the mass concentration of the sodium chloride solution is 10% to 30%, and the soaking time is 20 to 40 minutes.

7. The Eucheuma complex for improving lung function according to claim 4, characterized in that In step S2, the amount of water added is 20 to 30 times that of Eucheuma.

8. The Eucheuma complex for improving lung function according to claim 4, characterized in that In step S2, the extraction temperature is 85-90°C, and the extraction time is 1-2h.

9. The method for preparing the Eucheuma complex for improving lung function according to claim 2, characterized in that: The following steps are involved: (1) Take Ophiopogon japonicus and Citrus aurantium, add 95% v / v ethanol solution according to the solid-liquid ratio of 1:8-12 kg / L, reflux extraction for 2-4 hours, repeat the extraction 2-3 times, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain a composite extract; (2) taking Polygonatum sibiricum, Cistanche deserticola, Euryale ferox, and Longan, adding water at a material-liquid ratio of 1:14-16 kg / L, and boiling, and collecting the decoction; (3) Add the composite extract and the Eucheuma extract to the decoction, and finally add the oyster peptide and the fish collagen peptide, homogenize, and sterilize.

10. The method for preparing the Eucheuma complex for improving lung function according to claim 9, characterized in that: Step (1), each reflux extraction time is 2-4h; Step (2), the decoction time is 1-2h; In step (3), the homogenization pressure is 25-35 MPa and the homogenization time is 5-10 min.

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