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Maize oligopeptide and method for preparing same
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A technology of corn oligopeptide and corn, applied in the biological field, can solve the problem of not explaining the molecular weight distribution data of corn peptide crude protein content, etc.
Active Publication Date: 2009-09-16
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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Abstract
Description
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Examples
Experimental program
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Effect test
Embodiment 1
[0016] The first step is to take 150g of corn gluten powder, add 2.5Kg of water, stir and mix evenly;
[0017] The second step is to adjust the feed liquid to pH8 with NaOH solution, heat the feed liquid to 60°C, and keep stirring for 60 minutes;
[0018] The third step is to centrifuge the feed liquid, discard the clear night, and keep the slag;
[0019] The fourth step is to add 2.5Kg of water to the slag, stir and mix evenly, and keep stirring at 60°C for 60 minutes;
[0020] The fifth step is to centrifuge the feed liquid, discard the clear liquid, and keep the slag;
[0021] The sixth step is to add 2Kg of water to the slag, stir and mix evenly;
[0022] The seventh step is to adjust the feed liquid to pH8 with NaOH solution, heat the feed liquid to 60°C, then add 1g of alkaline protease, and enzymatically hydrolyze for 3 hours;
[0023] The eighth step is to adjust the feed liquid to pH 7 with HCL solution, lower the temperature of the feed liquid to 45°C, then add 1 ...
Embodiment 2
[0030] The first step is to take 1500g of corn gluten powder, add 15Kg of water, stir and mix evenly;
[0031] The second step is to adjust the feed liquid to pH 10 with NaOH solution, heat the feed liquid to 80°C, and keep stirring for 40 minutes;
[0032] The third step is to centrifuge the feed liquid, discard the clear night, and keep the slag;
[0033] The fourth step is to add 15Kg water to the slag material, keep stirring at 80°C for 40min;
[0034] The fifth step is to centrifuge the feed liquid, discard the clear night, and collect the slag;
[0035] The sixth step is to add 25Kg of water to the slag, stir and mix evenly;
[0036] The seventh step is to adjust the feed liquid to pH8.5 with NaOH solution, heat the feed liquid to 55°C, then add 15g of alkaline protease, and enzymatically hydrolyze for 4 hours;
[0037] The eighth step is to adjust the feed liquid to pH 7.5 with HCL solution, lower the temperature of the feed liquid to 50°C, then add 15g of neutral pr...
Embodiment 3
[0044] The corn oligopeptide powder that makes in example one, two is analyzed and detected, and its crude protein content 87.3%, fat content 1.5%, molecular weight distribution is as follows:
[0045] Molecular weight rangePeak Area Percentage Number average molecular weight Weight average molecular weight >3000 0.27 3455 3511 3000～2000 2.88 2280 2304 2000～1000 16.80 1288 1337 1000～500 41.91 707 736 500～130 29.54 269 312 <130
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Abstract
The invention relates to a maize oligopeptide and a method for preparing same, belonging to the field of biological technology. The method takes the byproduct, namely corn proteinpowder (also called maize yellow powder containing about 60% of protein) in corn starch production as a raw material, removes the impurities such as starch, grease and the like by the treatment of alkaline water and obtains high-purity corn proteinslag; furthermore, the corn protein slag is taken as enzymatic hydrolysis substrate and treated by the following process of pre-treatment, enzymatic hydrolysis, multi-step separation and purification, concentrating, drying and the like so as to prepare the maize oligopeptide powders with edible value and various health-care functions. The method for preparing maize oligopeptide has the advantages of easily obtaining raw material, simple process and higher industrial feasibility. The prepared maize oligopeptide sample has high crude protein content and small molecular weight, has various physiological activities of prompting immunity adjustment, anti-fatigue and the like, and can be used as new functional nutritional compounding and widely applied to the development and production of products such as foods, health-care foods and the like.
Description
technical field [0001] The invention relates to using corn glutenpowder as a raw material to prepare high-purity corn oligopeptide powder through protein purification, enzymatic hydrolysis, separation and refining, and belongs to the field of biotechnology. Background technique [0002] As one of the three major food crops, corn occupies an important position in the world's food structure. At present, China's annual corn production is about 110 million tons, accounting for 20% of the world's total production, of which nearly 4 million tons are used to produce starch, starchsugar and alcohol. Corn gluten powder, commonly known as yellow powder, is a by-product of corn starch. The protein contained in it lacks essential amino acids such as lysine and tryptophan, so its biological value is low and has not been well utilized. [0003] Corn gluten powder is the main by-product of corn wet processing starch. It is made from coarse starch milk obtained by wet milling process, th...
Claims
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Application Information
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IPC IPC(8): C07K1/14C07K2/00C12P21/06
Inventor 蔡木易易维学谷瑞增马勇董哲郭晖马永庆
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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