Method for breaking walls of cells of bran by wet grinding

A technology of wet pulverization and cell wall, applied to plant cells, etc., can solve the problems of insufficient dissolution of cytoplasm, low cell wall breaking rate, high temperature rise, etc., and achieve the goal of eliminating cellulose crystal structure, improving enzymatic hydrolysis efficiency, and reducing energy consumption Effect

Inactive Publication Date: 2010-12-08
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The ultrafine pulverization technology in the prior art mainly uses high-speed airflow pulverization and grinding to break the cell wall, but the disadvantages of this pulverization method are high energy consumption and low efficiency; the pulverization process has a high temperature rise and has a great impact on the active ingredients ; The cell wall breaking rate is low, and the cytoplasmic dissolution is not sufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water twice the mass of the material, add 2 u / g of cellulase, and put it in a planetary ball mill (XQM-20 L, Nanjing Science and Technology Co., Ltd. The milling speed was 500 rpm, and the temperature was controlled not to exceed 50°C during the pulverization process. After pulverization for 5 hours, the wheat bran and agate balls were separated without drying to obtain the wheat bran wall-broken powder. The measured median diameter was 4.75 μm, and the cell wall breaking rate was 99.2%.

Embodiment 2

[0024] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water 5 times the mass of the material, add hemicellulase 10 u / g, and put it in a planetary ball mill (XQM-20 L, Nanjing Keyan Experimental Instrument Research Institute) medium ball milling, the ball milling speed is 425 rpm, the temperature is controlled not to exceed 50°C during the pulverization process, after pulverization for 3 hours, the wheat bran and agate balls are separated without drying, and the wheat bran broken wall powder is obtained . The measured median diameter was 3.55 μm, and the cell wall breaking rate was 99.2%.

Embodiment 3

[0026] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water 9 times the mass of the material, add a compound enzyme mixed with cellulose and hemicellulase, cellulase and hemicellulase The amount of each was 10 u / g, and they were ball-milled in a planetary ball mill (XQM-20 L, Nanjing Keyan Experimental Instrument Research Institute), the milling speed was 350 rpm, the temperature was controlled not to exceed 50°C during the pulverization process, and after 1 h of pulverization, , after the wheat bran is separated from the agate balls, no drying is required to prepare the wheat bran wall breaking powder. The median diameter was 3.05 μm, and the cell wall breaking rate was 99.7%.

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Abstract

The invention relates to a method for breaking walls of cells of bran by wet grinding, and belongs to the technical field of industrial biochemistry. The invention adopts the technical key point that: bran, water, and enzyme for degrading walls of cells of the bran are mixed, and subjected to wet ball-milling grinding to form superfine wall-breaking powder. By combining an ultramicro-pulverization method, the water and the enzyme for degrading the walls of cells of the bran, the cells of the bran are subjected to wet ball milling for wall-breaking treatment; compared with single ultramicro pulverization, the method can greatly reduce the energy consumption, is simple and has low requirement on operating equipment; and compared with single enzymatic degradation, the method combines wet ball milling, combines chemical action with mechanical force action, and has the advantages of greatly saving energy required by high temperature treatment, avoiding the defect of enzyme inactivation under high temperature treatment, eliminating crystal structures of cellulose and improving enzymolysis efficiency.

Description

technical field [0001] The invention relates to a method for wet crushing and wall breaking of bran cells, which belongs to the technical field of industrial biochemical industry. Background technique [0002] Bran contains natural phytoestrogens such as xylem and isoflavones. These natural phytoestrogens can suppress cancer and lower cholesterol, and phytoestrogens in bran can be "reprocessed" by intestinal bacteria to make plants The total amount of estrogen can be increased by about 10 times. Therefore, it is of great significance to carry out research on the deep processing of bran and the effect of phytoestrogens. However, the nutrients in the bran are mainly concentrated in the bran cell wall, and the bran cell wall is highly fibrous and very tough. Therefore, in order to utilize the above nutrients in the bran, the structure of the bran cell wall must be fully decomposed first. [0003] The main components of plant cell walls are cellulose, hemicellulose and pectin....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 吴琴燕杨敬辉庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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