Method for preparing fumaric acid fermentation liquor by fermenting corncob cellulose

A corn cob and cellulose technology, applied in the field of fermentation, can solve the problems of low fermentation sugar yield and achieve the effects of loose cellulose structure, easy hydrolysis and improved purity

Inactive Publication Date: 2014-05-14
HEFEI UNIV OF TECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing fumaric acid fermentation liquid by fer...

Method used

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preparation example Construction

[0018] The preparation of cellulase refers to "Gao Xingxing, Pan Lijun, Yang Peizhou, Wang Xiuyang. Condition optimization of mixed fermentation of Trichoderma reesei and Aspergillus niger to produce cellulase [J]. Food Science, 2012, 33(19):193-198 ; " method, concrete preparation process is as follows:

[0019] (1) Strains: Trichoderma reesei (Trichoderma ressei CGMCC3.03711) was purchased from China General Microorganism Culture Collection and Management Center, and Aspergillus niger CICC41254 was purchased from China Industrial Microbiology Culture Collection and Management Center.

[0020] Raw materials: corn stalks, purchased from Feidong County, Hefei City, Anhui Province, crushed to 40 mesh, soaked in alkaline hydrogen peroxide solution for 30 hours, washed with tap water until neutral, dried in an oven, and used as a substrate for fermentation enzyme production.

[0021] (2) Strain preservation slant medium: potato medium (PDA).

[0022] (3) Mandel’s mycelium culture...

Embodiment 1

[0031] The specific operation steps of using corncob prehydrolysis and synchronous fermentation to produce fumaric acid at the scale of 10L fermenter are as follows:

[0032] (1) After crushing 1.5kg of sun-dried corn cobs to 40 meshes, add them to a semi-automatic heating filter tank with 15kg of 3% hydrogen peroxide and 1.5mol / L NaOH alkaline hydrogen peroxide solution and mix evenly. Adjust the temperature to 90°C for 18 hours for pretreatment. After treatment, alkaline hydrogen peroxide is recovered through a filter device, and the filter residue is washed to neutrality, and dried to obtain pretreated corncob cellulose.

[0033] (2) Weigh 1kg of the pretreated corncob cellulose, add it into a 10L fermenter, and sterilize it at 121°C for 15min. When cooled to 50°C, add 820mL of cellulase solution and 8.18L of sterile water into the fermenter, control the temperature at 50°C, stir at 120r / min, and pre-hydrolyze for 16h.

[0034] (3) After the pre-hydrolysis is completed, l...

Embodiment 2

[0036] The specific operation steps of using corncob prehydrolysis and synchronous fermentation to produce fumaric acid in a 500L scale fermentation tank are as follows:

[0037] (1) Crush 75kg of sun-dried corn cobs to 40 meshes, add them to a semi-automatic heating filter barrel filled with 750kg of 3% hydrogen peroxide and 1.5mol / L NaOH alkaline hydrogen peroxide solution, and mix well, and adjust the temperature to 90°C , the time is 18h, carry out pretreatment. After treatment, alkaline hydrogen peroxide is recovered through a filter device, and the filter residue is washed to neutrality, and dried to obtain pretreated corncob cellulose.

[0038] (2) Weigh 50kg of corncob cellulose, put it into a 500L fermenter, and sterilize it at 121°C for 15min. When cooled to 50°C, add 41L of cellulase solution and 409L of sterile water to the fermenter, control the temperature at 50°C, stir at 120r / min, and pre-hydrolyze for 16h.

[0039] (3) After the pre-hydrolysis is completed, ...

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Abstract

The invention provides a method for preparing fumaric acid fermentation liquor by fermenting corncob cellulose. After corncob is pretreated by using alkaline hydrogen peroxide, lignin and hemicellulose in the corncob can be removed effectively, the purity of the cellulose is increased, the cellulose is loose in structure and easier to hydrolyze, and the loss ratio of the corncob is reduced to 26.85-27.16 percent. On the basis, the pretreated corncob raw material is subjected to pre-enzymolysis, a part of the cellulose in the raw material is converted into small-molecular sugar, a rhizopus delemar seed solution is introduced, enzymolysis and fermentation are performed synchronously, glucose is converted into fumaric acid continuously in an enzymolysis process, the feedback inhibition action of a large amount accumulated glucose on enzyme in a subsequent enzymolysis process is relieved, and the cellulose conversion rate and the fumaric acid yield are increased. According to the method, the corncob is taken as a raw material, so that a novel way is opened up for the production of the fumaric acid, and the economical significance of industrial microorganism production is great.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for preparing fumaric acid fermentation liquid by fermenting corn cob cellulose. Background technique [0002] The main components of cellulosic resources are fiber, hemicellulose and lignin. Among them, cellulose and hemicellulose are the sources of fermentable sugars, and their content accounts for 66-75% of lignocellulose (the dry weight of cellulosic raw materials). The structure of the cellulosic raw material is very complex and must undergo certain treatment to degrade it into small molecular sugars, which are then fermented into fumaric acid by Rhizopus dell's. [0003] The initial preparation of organic acids by enzymatic hydrolysis and fermentation of cellulose is carried out in steps of enzymatic hydrolysis and fermentation. When the natural lignocellulose raw materials are directly subjected to enzymatic hydrolysis after pretreatment, due to ...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/845
Inventor 李兴江李硕姜绍通潘丽军吴悦刘亚孙婷
Owner HEFEI UNIV OF TECH
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