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Composite enzymolysis reagent for keratin enzymolysis and application method thereof

A keratinase and compound enzymolysis technology, applied in the field of proteolysis, can solve the problems of low dissolution efficiency of reagents, difficulty in reuse, great health risks, etc., so as to avoid the generation of free radicals and toxic substances, and increase the content of protein powder. , the effect of mild reaction conditions

Active Publication Date: 2015-06-03
湖州珍贝羊绒制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods mainly have the following problems: a large amount of chemical reagents are added in the process of hair dissolution, the dissolution efficiency of these reagents is not high, it is difficult to reuse and cause certain pollution to the environment; in addition, the traditional cutin hydrolysis conditions, the temperature is 85 ° C Above, pH close to 12, such conditions are the source of a large number of free radicals and toxic substances, the resulting keratin products often contain more bacteria and toxic substances, if directly applied to food, there will be a large health risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The method for preparing protein powder by enzymolysis of keratin comprises the following steps:

[0056] 1) Degreasing

[0057] Using mohair as raw material, the cleaned mohair is first degreased;

[0058] 2) Enzymolysis

[0059] Take 3.5 g / L of the above composite enzymatic hydrolysis reagent A, adjust the pH value to 4, add defatted mohair to it, pretreat at room temperature and use helium to cut off oxygen for 25 minutes, then adjust the pH value to 6.5, and control the temperature to 60°C. Enzymolysis for 12 hours to obtain an enzymolysis solution;

[0060] 3) The enzymatic solution is desalted by ceramic micro-pore microfiltration;

[0061] 4) Sterilize and dry.

[0062] After testing, the degradation rate of keratin is 94.5%, and the protein content in protein powder is 94.1%.

Embodiment 2

[0064] 1) Degreasing

[0065] Using wool as raw material, the cleaned wool is first degreased;

[0066] 2) Enzymolysis

[0067] Take 5.2 g / L of the above compound enzymolysis reagent B, adjust the pH value to 5, add degreased wool to it, pretreat at room temperature and use helium to isolate oxygen for 25 minutes, then adjust the pH value to 7.5, and control the temperature to 50°C. Enzymolysis for 24 hours to obtain an enzymolysis solution;

[0068] 3) The enzymatic solution is desalted by ceramic micro-pore microfiltration;

[0069] 4) Sterilize and dry.

[0070] After testing, the degradation rate of keratin was 94.2%, and the protein content in protein powder was 93.0%.

Embodiment 3

[0072] 1) Degreasing

[0073] Using light-colored alpaca as raw material, the cleaned light-colored alpaca is first degreased;

[0074] 2) Enzymolysis

[0075] Use the above compound enzymolysis reagent C6.3 g / L, adjust the pH value to 4.5, add defatted light-colored alpaca hair to it, pretreat at room temperature and use helium to isolate oxygen for 20 minutes, then adjust the pH value to 7, and control the temperature 55°C, enzymatic hydrolysis for 20 hours to obtain the enzymatic hydrolysis solution;

[0076] 3) The enzymatic solution is desalted by ceramic micro-pore microfiltration;

[0077] 4) Sterilize and dry.

[0078] After testing, the degradation rate of keratin is 95.1%, and the protein content in protein powder is 93.8%.

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Abstract

The invention discloses a composite enzymolysis reagent for keratin enzymolysis and an application method thereof. The composite enzymolysis reagent comprises streptomycete cutin protease, yeast enzyme and a reducing agent. The application method comprises the following steps: firstly, performing ungrease treatment on a natural raw material with cornifin, a yeast enzyme and a reducing agent; adding the natural raw material with the cornifin after ungrease treatment in the step 1) into a combined enzymolysis agent liquid, pretreating for 15-25 minutes at the room temperature without oxygen, further adjusting the pH value of the combined enzymolysis agent liquid to be 7.0-7.5, controlling the temperature to be 50-60 DEG C, and performing enzymolysis for 12-24 hours, thereby obtaining a keratinase liquid; decoloring and sterilizing the keratinase liquid; and performing vacuum drying, thereby obtaining protein powder. The composite enzymolysis reagent is rapid in enzymolysis and high in keratin degradation rate, and generation of toxic substances can be effectively prevented, so that the prepared protein powder is safe and non-toxic and high in nutrition component.

Description

technical field [0001] The invention belongs to the technical field of proteolysis, and in particular relates to a compound enzymolysis reagent for enzymolysis of keratin and an application method. Background technique [0002] Humans have a long history of processing and utilizing natural keratin, among which the earliest plastic products are made of keratin. Biodegradable plastics and biomedical materials are getting more and more attention. One of the research hotspots is the extraction of keratin from hair and poultry feathers. There are two main types of wool keratin processing and extraction methods: mechanical and chemical. The mechanical method of preparing keratin generally uses heating (100-200°C) and pressure (0.3-1.0Mpa) to destroy the disulfide bonds and peptide chains inside the keratin, making it into a soluble and easily digestible polypeptide Mixture, mostly used in feed industry. The chemical method is more mature hair hydrolysis method, mainly includin...

Claims

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Application Information

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IPC IPC(8): C12N9/52A23J3/34A23J3/04
CPCA23J3/04A23J3/341C12N9/52
Inventor 武达机邱金元龚熠姚春梅
Owner 湖州珍贝羊绒制品有限公司
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