Efficient combined preprocessing method for straws

A pretreatment and straw technology, applied in the direction of fermentation, etc., can solve the problems of hindering the enzymatic hydrolysis of cellulose, inability to remove lignin, unfavorable enzymatic hydrolysis efficiency, etc., to promote enzymatic hydrolysis, shorten ball milling time, and improve enzymatic hydrolysis efficiency Effect

Active Publication Date: 2014-11-05
JIANGNAN UNIV
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Problems solved by technology

However, this type of method is a high-energy pretreatment method and needs to be combined with other pretreatment methods to achieve energy saving and cost reduction.
Moreover, lignin cannot be removed by this type of method, and the existence of lignin will adsorb part of cellulase on the one hand, and hinder the enzymatic hydrolysis of cellulose by cellulase on the other hand as a physical barrier, which is not conducive to the further improvement of enzymatic hydrolysis efficiency. improve

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  • Efficient combined preprocessing method for straws
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  • Efficient combined preprocessing method for straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Combination Pretreatment Scheme 1 of Corn Stalk Ozone-Ball Milling

[0034] Coarsely pulverize the corn stalks with a pulverizer, pass the pulverized material through a 30-mesh sieve, and take the sieved material. Add 1 times (v / w) deionized water to the undersieve, mix well, and then pass ozone treatment, the ozone concentration is 60 mg / L, the ozone flow rate is 1.0 L / h, and the ozone treatment time is 2h; the ozone The treated straw was dried naturally, and then added to the ball mill tank, the mass ratio of straw to ball was 1:20, the speed of the ball mill was 1116 rpm, and the ball milled for 24 min.

[0035] Add 20 times (v / w) deionized water to the straw after combined treatment, mix well and let it stand for 0.5 h, then collect the solid by filtration to remove small molecular organic acids and other substances; in the straw after washing and removing acid Add 20 times (v / w) sodium citrate buffer at pH 4.8, pre-added complex cellulase in the buffer,...

Embodiment 2

[0038] Example 2: Combination Pretreatment Scheme 2 of Corn Stalk Ozone-Ball Milling

[0039]Coarsely pulverize the corn stalks with a pulverizer, pass the pulverized material through a 30-mesh sieve, and take the sieved material. Add 0.4 times (v / w) deionized water to the undersieve, mix well, and then pass ozone treatment, the ozone concentration is 20 mg / L, the ozone flow rate is 2.0 L / h, and the ozone treatment time is 0.5 h; The straw after ozone treatment was dried in vacuum, and then added to the ball mill tank, the mass ratio of straw to ball was 1:10, the speed of ball mill was 279 rpm, and the ball mill was milled for 1 min.

[0040] Add 5 times (v / w) deionized water to the straw after combined treatment, mix well and let it stand for 0.5 h, then collect the solid by suction filtration to remove small molecular organic acids and other substances; the straw after washing with water Add 5 times (v / w) sodium citrate buffer solution with pH 4.8 to the buffer, pre-added ...

Embodiment 3

[0043] Example 3: Combination Pretreatment Scheme 1 of Corn Stalk Milling-Ozone

[0044] Coarsely pulverize the corn stalks with a pulverizer, pass the pulverized material through a 30-mesh sieve, and take the undersieve; put the undersieve into the ball mill tank, the mass ratio of the straw to the ball is 1:20, the speed of the ball mill is 558 rpm, and the ball mill 2 min; take the ball-milled straw, add 1 times (v / w) deionized water, mix well, and then pass ozone treatment, the ozone concentration is 80 mg / L, the ozone flow rate is 0.5 L / h, the ozone treatment time for 1.5 h.

[0045] Add 15 times (v / w) deionized water to the straw after combined treatment, mix well and let it stand for 1 hour, then collect the solid by centrifugation to remove small molecular organic acids and other substances; in the straw after washing and removing acid Add 15 times (v / w) sodium citrate buffer solution with pH 4.8, pre-added compound cellulase in buffer, compound cellulase contains cel...

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Abstract

An efficient combined preprocessing method for straws employs an ozone processing method combined with a ball milling method to perform efficient combined pretreatment on straws, and concretely comprises two parts: (1) firstly performing ozone pretreatment on crushed straws, and then performing ball milling pretreatment; and (2) firstly performing ball milling pretreatment on crushed straws and then performing ozone pretreatment. After straws are subjected to combined pretreatment, water-washing acid removal and compound cellulase enzymolysis, the glycation rate is substantially improved.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for effectively combining ozone and ball milling to pretreat straw. Background technique [0002] Lignocellulose is the most abundant and cheap renewable resource. my country produces more than 700 million tons of crop straw every year, [0003] Among them, 200 million tons were incinerated on the spot, which not only caused a great waste of resources, but also seriously polluted the environment and caused fire hazards. Therefore, how to efficiently use these lignocelluloses has become a research hotspot. Relevant literature has reported that straw-based lignocellulose has great application prospects in the fields of bioenergy, biofeed, and biofertilizer. In terms of bioenergy, it can replace food crops as raw materials for the production of second-generation fuel ethanol, and can produce ethanol after enzymatic saccharification and fermentation of Saccharomyces c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 史锋李永富项和济
Owner JIANGNAN UNIV
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