Lignocellulose pretreatment method for increasing enzyme hydrolysis rate by oxidation removal of lignin

A lignocellulose and pretreatment technology, applied in the direction of fermentation, etc., can solve the problems of less research literature, destroy the recalcitrant structure of biomass, destroy the crystal structure, etc., and achieve the improvement of xylose yield, simple and easy operation, clean and efficient separation Effect

Inactive Publication Date: 2017-09-15
TIANJIN UNIV OF SCI & TECH
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  • Application Information

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Problems solved by technology

[0004] At present, among the commonly used lignocellulose pretreatment methods (ionic liquid method, acid-base pretreatment method, steam explosion method, ozone pretreatment method, etc.), the combination of alkaline reagents and strong oxidants has been reported to effectively remove the The lignin in the material can destroy the hydrogen bond between hemicellulose and cellulose and the crystal structure of cellulose at the same time, thereby destroying the recalcitrant structure in biomass, but there are few related research literatures

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  • Lignocellulose pretreatment method for increasing enzyme hydrolysis rate by oxidation removal of lignin
  • Lignocellulose pretreatment method for increasing enzyme hydrolysis rate by oxidation removal of lignin

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Embodiment 1

[0015] A pretreatment method for increasing the enzymatic hydrolysis rate of corn stalk raw materials by oxidative delignification, comprising the following steps:

[0016] (1) Crush the corn stalks, pass through a 60-mesh sieve and dry at 90°C until constant weight.

[0017] (2) Take 10 g of crushed and dried corn stalk raw material, add 100 mL of 4% (w / v) sodium percarbonate solution, and treat at 60° C. for 4 h.

[0018] (3) Filter the pretreated corn stalks, wash with distilled water until the pH of the filtrate reaches neutral, and dry at 90° C. to constant weight.

[0019] (4) Take 2.0 g of the dried corn stalk residue (or corn stalk raw material without pretreatment) after the above-mentioned pretreatment, add 25 mL of 0.05 mol / L, pH to 4.8 citric acid buffer solution, add to the mixture Add commercial cellulase and xylanase derived from Trichoderma, wherein the loading of cellulase is 30FPU / g substrate, and the loading of xylanase is 8000U / g substrate, and the total v...

Embodiment 2

[0024] A pretreatment method for improving enzymatic hydrolysis rate of corn cob raw material by oxidative delignification, comprising the following steps:

[0025] (1) The corncob raw material was crushed, passed through an 80-mesh sieve, and then dried at 90°C to constant weight.

[0026] (2) Take 10 g of crushed and dried corn cob raw material, add 100 mL of 5% (w / v) sodium percarbonate solution, and treat at 50° C. for 6 h.

[0027] (3) Filter the pretreated corncobs, wash with distilled water until the pH of the filtrate reaches neutrality, and dry at 90° C. to constant weight.

[0028] (4) Take 2.0 g of the dried corncob residue (or corncob raw material without pretreatment) after the above-mentioned pretreatment, add 25mL of 0.05mol / L, pH to 4.8 citric acid buffer solution, add to the mixture Add commercial cellulase and xylanase derived from Trichoderma, wherein the loading amount of cellulase is 40FPU / g substrate, and the loading amount of xylanase is 10000U / g substr...

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Abstract

The invention relates to a lignocellulose pretreatment method for increasing an enzyme hydrolysis rate by oxidation removal of lignin. The method includes steps: grinding and drying lignocellulose raw materials, respectively controlling sodium percarbonate concentration, solid-liquid ratio, pretreatment temperature and time in appropriate ranges, filtering, washing to neutral, and drying to obtain pretreated lignocellulose residues; adding cellulase and xylanase, dissolved in citric acid buffer solution with pH in a range of 4.5-5.0, into the pretreated lignocellulose residues to obtain fermentable reducing sugar solution through enzymolysis. By the pretreatment method, lignin can be effectively removed while the retaining rate of cellulose and hemicellulose components is high, lignocellulose enzymolysis efficiency is effectively improved, and reducing sugar yield is increased. In addition, the method has advantages of environmental friendliness, simplicity in operation, low cost and the like.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of lignocellulosic biomass, and in particular relates to a pretreatment method for improving the enzymatic hydrolysis rate of lignocellulosic biomass by using a strong oxidant to oxidize lignification. Background technique [0002] Under the dual pressure of economic development and environmental protection, the development and utilization of renewable resources such as biomass is an effective measure for countries all over the world to establish sustainable energy systems. Lignocellulosic biomass is the largest and most potential renewable resource in nature, and its effective transformation and utilization is of inestimable value to the sustainable development of human beings. At present, the hydrolysis of lignocellulose into reducing sugars by biological methods and then microbial fermentation to obtain the required products has become a research hotspot and main research direction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14
CPCC12P19/02C12P19/14C12P2201/00
Inventor 马立娟杜丽平肖冬光马清郭高杰崔有志
Owner TIANJIN UNIV OF SCI & TECH
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