Method for producing ethanol from tobacco stems by fermentation

A technology for tobacco stalks and raw materials, which is applied in the field of ethanol production by using tobacco stalk raw materials by fermentation, can solve the problems of waste of agricultural residual resources and environmental pollution, and achieve the effects of rational development and utilization, reduction of environmental pollution and simple operation process.

Active Publication Date: 2015-07-01
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the harvesting of tobacco leaves in our country every year, a large amount of low-grade tobacco leaves, tobacco stalks, fireworks and tobacco roots and other wastes will be produced. These wastes are either discarded in the field or dried and burned, which not only causes serious pollution to the environment , but also a waste of agricultural surplus resources

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  • Method for producing ethanol from tobacco stems by fermentation
  • Method for producing ethanol from tobacco stems by fermentation

Examples

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Effect test

Embodiment 1

[0021] A method for producing ethanol by using tobacco stalk raw materials by fermentation includes the following steps:

[0022] ① Pretreatment of raw materials: crush the tobacco stalks to 10 meshes, and then subject the smashed tobacco stalks to steam explosion pretreatment. The burst pressure is 2.0 MP, the pressure holding time is 2.5 minutes, and then the pressure is released to normal pressure instantly;

[0023] ②Enzymatic hydrolysis and saccharification of materials: add water to the tobacco stalk pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of the tobacco stalk and the added water is 1:8 (kg / L); Adjust the pH value of the solid-liquid mixture with 10 mol / L sodium hydroxide to 4.8, then add cellulase and xylanase, hydrolyze the enzyme in a shaker with 120 r / min at 48°C for 48 hours, and filter to obtain the hydrolysate. ; The amount of cellulase added is 15FPU / g tobacco straw, and the amount of xylanase added i...

Embodiment 2

[0036] A method for producing ethanol by using tobacco stalk raw materials by fermentation includes the following steps:

[0037] ①Pretreatment of raw materials: crush the tobacco stalks to 50 meshes, and then subject the smashed tobacco stalks to steam explosion pretreatment. The burst pressure is 2.5MP, the pressure holding time is 1min, and then the pressure is released to normal pressure instantly;

[0038] ②Enzymatic hydrolysis and saccharification of materials: Add water to the tobacco stalk pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of the tobacco stalk and the added water is 1:5 (kg / L); Adjust the pH value of the solid-liquid mixture with 10 mol / L sodium hydroxide to 4.5, then add cellulase and xylanase, hydrolyze the enzyme for 36 hours at 45℃, shaker at 120 r / min, and filter to obtain the hydrolysate , Where the addition amount of cellulase is 50 FPU / g tobacco straw, and the addition amount of xylanase is 50...

Embodiment 3

[0045] A method for producing ethanol by using tobacco stalk raw materials by fermentation includes the following steps:

[0046] ①Pretreatment of raw materials: crush the tobacco stalks to 100 meshes, and then subject the smashed tobacco stalks to steam explosion pretreatment. The burst pressure is 1.5 MP, the pressure holding time is 5 minutes, and then the pressure is released to normal pressure instantly;

[0047] ②Enzymatic hydrolysis and saccharification of materials: Add water to the tobacco stalk pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of the tobacco stalk and the added water is 1:10 (kg / L); Adjust the pH value of the solid-liquid mixture with 10 mol / L sodium hydroxide to 4.5, then add cellulase and xylanase, hydrolyze the enzyme for 48 hours at 45℃, shaker at 120 r / min, and filter to obtain the hydrolysate ; Among them, the addition amount of cellulase is 30 FPU / g tobacco straw, and the addition amount of ...

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Abstract

The invention belongs to a method for producing ethanol from tobacco stems by fermentation; a fibrous raw material is processed under the action of high-temperature and high-pressure steam by adopting a steam explosion method; the component separation of the raw material is realized through a sudden depressurizing process; therefore, cellulose, hemicellulose and lignin in the material are degraded and dissolved out; cellulose is easily subjected to emzymolysis so as to be converted into sugar; and then, fermentation of cellulosic ethanol is carried out by selectively using an issatchenkia orientalis strain. The pre-processing method can be applied to various agricultural wastes, such as bagasse, wheat straws, rice straws, corn stalks and corncobs; the enzymatic hydrolysis ratio of cellulose can be obviously increased; ethanol is prepared by fermentation by taking tobacco wastes as the raw materials; therefore, the operation process is simple; the production cost is low; furthermore, the agricultural wastes can be changed into wealth; environmental pollution is reduced; and reasonable development and utilization of agricultural surplus resources are realized.

Description

Technical field [0001] The invention belongs to the technical field of agricultural biomass energy, and specifically relates to a method for producing ethanol by using tobacco straw raw materials to ferment. Background technique [0002] Tobacco is one of the important economic crops in my country and has special economic value. my country's tobacco planting area and output rank first in the world, and tobacco planting and processing play an important role in the national economy. However, every year after the harvest of tobacco leaves in our country, a large amount of wastes such as low-grade tobacco, tobacco stalks, fireworks and tobacco roots are generated. These wastes are either discarded in the field or dried and burned, which not only causes serious pollution to the environment , And it is also a waste of agricultural surplus resources. In recent years, the domestic comprehensive utilization of tobacco waste is mainly the analysis and extraction of nicotine, solanesol, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCC12P7/10Y02E50/10
Inventor 王风芹宋安东付晨青任天宝谢慧杨森张炎达
Owner HENAN AGRICULTURAL UNIVERSITY
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