A method for producing ethanol by fermenting raw materials of tobacco stalks

A tobacco stalk and raw material technology, applied in the field of ethanol production by fermenting tobacco stalk raw materials, can solve the problems of waste of agricultural surplus resources and environmental pollution, and achieve the effects of rational development and utilization, reduction of environmental pollution, and simple operation process

Active Publication Date: 2018-02-16
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the harvesting of tobacco leaves in our country every year, a large amount of low-grade tobacco leaves, tobacco stalks, fireworks and tobacco roots and other wastes will be produced. These wastes are either discarded in the field or dried and burned, which not only causes serious pollution to the environment , but also a waste of agricultural surplus resources

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  • A method for producing ethanol by fermenting raw materials of tobacco stalks
  • A method for producing ethanol by fermenting raw materials of tobacco stalks

Examples

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Effect test

Embodiment 1

[0021] A method for producing ethanol by fermenting raw materials of tobacco stalks, comprising the following steps:

[0022] ① Pretreatment of raw materials: crush the tobacco stalks to 10 meshes, and then perform steam explosion pretreatment on the crushed tobacco stalks. The explosion pressure is 2.0 MP, the pressure holding time is 2.5 minutes, and then the pressure is released to normal pressure instantly;

[0023] ② Enzymatic hydrolysis and saccharification of materials: add water to the tobacco stalks pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of tobacco stalks and added water is 1:8 (kg / L); 10 mol / L sodium hydroxide was used to adjust the pH value of the solid-liquid mixture to 4.8, then cellulase and xylanase were added, and at 48°C, 120 r / min shaker was used for enzymolysis for 48 hours, and then filtered to obtain the enzymolysis solution ; Wherein the addition amount of cellulase is 15 FPU / g tobacco s...

Embodiment 2

[0036] A method for producing ethanol by fermenting raw materials of tobacco stalks, comprising the following steps:

[0037] ①Pretreatment of raw materials: crush the tobacco stalks to 50 meshes, and then perform steam explosion pretreatment on the crushed tobacco stalks. The blasting pressure is 2.5MP, the pressure holding time is 1min, and then the pressure is released to normal pressure instantly;

[0038] ② Enzymatic hydrolysis and saccharification of materials: add water to the tobacco stalks pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of tobacco stalks and added water is 1:5 (kg / L); 10 mol / L sodium hydroxide was used to adjust the pH value of the solid-liquid mixture to 4.5, then cellulase and xylanase were added, and at 45°C, 120 r / min shaker was shaken for 36 hours, and filtered to obtain the enzymolysis solution , wherein, the addition amount of cellulase is 50 FPU / g tobacco stalk, and the addition amoun...

Embodiment 3

[0045] A method for producing ethanol by fermenting raw materials of tobacco stalks, comprising the following steps:

[0046] ①Pretreatment of raw materials: crush the tobacco stalks to 100 mesh, and then perform steam explosion pretreatment on the crushed tobacco stalks, the blasting pressure is 1.5 MP, the pressure holding time is 5 minutes, and then the pressure is released to normal pressure instantly;

[0047] ② Enzymatic hydrolysis and saccharification of materials: add water to the tobacco stalks pretreated by steam explosion, mix, and stir to form a uniform solid-liquid mixture. The solid-liquid ratio of tobacco stalks and added water is 1:10 (kg / L); 10 mol / L sodium hydroxide was used to adjust the pH value of the solid-liquid mixture to 4.5, then cellulase and xylanase were added, and at 45°C, 120 r / min shaker was used for enzymolysis for 48 hours, and then filtered to obtain the enzymolysis solution ; Wherein, the addition amount of cellulase is 30 FPU / g tobacco stal...

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Abstract

The invention belongs to a method for producing ethanol from tobacco stems by fermentation; a fibrous raw material is processed under the action of high-temperature and high-pressure steam by adopting a steam explosion method; the component separation of the raw material is realized through a sudden depressurizing process; therefore, cellulose, hemicellulose and lignin in the material are degraded and dissolved out; cellulose is easily subjected to emzymolysis so as to be converted into sugar; and then, fermentation of cellulosic ethanol is carried out by selectively using an issatchenkia orientalis strain. The pre-processing method can be applied to various agricultural wastes, such as bagasse, wheat straws, rice straws, corn stalks and corncobs; the enzymatic hydrolysis ratio of cellulose can be obviously increased; ethanol is prepared by fermentation by taking tobacco wastes as the raw materials; therefore, the operation process is simple; the production cost is low; furthermore, the agricultural wastes can be changed into wealth; environmental pollution is reduced; and reasonable development and utilization of agricultural surplus resources are realized.

Description

technical field [0001] The invention belongs to the technical field of agricultural biomass energy, and in particular relates to a method for producing ethanol by fermenting tobacco stalk raw materials. Background technique [0002] Tobacco is one of the important economic crops in my country and has special economic value. my country's tobacco planting area and output rank first in the world, and tobacco planting and processing play an important role in the national economy. However, after the harvesting of tobacco leaves in our country every year, a large amount of low-grade tobacco leaves, tobacco stalks, fireworks and tobacco roots and other wastes will be produced. These wastes are either discarded in the field or dried and burned, which not only causes serious pollution to the environment , but also a waste of agricultural surplus resources. In recent years, the domestic comprehensive utilization of tobacco waste is mainly the analysis and extraction of nicotine, sol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10
CPCC12P7/10Y02E50/10
Inventor 王风芹宋安东付晨青任天宝谢慧杨森张炎达
Owner HENAN AGRICULTURAL UNIVERSITY
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