Phellinus igniarius mycelium liquid fermentation method by utilizing fungal elicitor for improving yield of flavone of phellinus igniarius

A technology of fungal elicitor and Phellinus mycelium, which is applied in the field of bioengineering to achieve the effect of increasing yield, remarkable effect and simple process

Inactive Publication Date: 2010-05-12
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of fungal elicitors for Phellinus fermented culture to increase the production of Phellinus flavonoids and other secondary metabolites has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Strain: the fungal elicitor strain is Acremonium strictum, purchased from China General Microorganism Culture Collection and Management Center, and the strain number is CGMCC No.3.2058.

[0024] Phellinus spp. is a common cultivar (Phellinus igniarius), which was purchased from the China General Microorganism Culture Collection and Management Center, and the number is CGMCC No.51328.

[0025] A method for liquid fermentation of Phellinus mycelium using fungal elicitors to increase the output of Phellinus flavonoids, characterized in that it comprises the following steps:

[0026] (1) Preparation of fungal elicitor:

[0027] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 25°C for 5 days, and use a puncher to punch out a diameter of 0.3 at the edge of the colony. Inoculate 3 pieces of bacterial block of cm in PD liquid medium, fill each 500mL Erlenmeyer flask wit...

Embodiment 2

[0036] Strain: The fungal inducer strain is Trichodermaviride, a fungus of the genus Trichoderma, purchased from China General Microorganism Culture Collection and Management Center, and the strain number is CGMCC No.3.2196.

[0037] Phellinus spp. is a common cultivar (Phellinus igniarius), purchased from China General Microorganism Culture Collection Center, No. CGMCC No.51328.

[0038] A method for liquid fermentation of Phellinus mycelium using fungal elicitors to increase the output of Phellinus flavonoids, characterized in that it comprises the following steps:

[0039] (1) Preparation of fungal elicitor:

[0040] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 30°C for 3 days, and use a puncher to punch out a diameter of 0.6 at the edge of the colony. Inoculate 5 pieces of bacterial blocks of cm, inoculate in PD liquid medium, fill each 500mL Erlenmeyer bottle wit...

Embodiment 3

[0049] Strain: the fungal elicitor strain is Verticillium psalliotae, purchased from China General Microorganism Culture Collection and Management Center, and the strain number is CGMCC No.3.4423.

[0050] Phellinus spp. is a common cultivar (Phellinus igniarius), which was purchased from the China General Microorganism Culture Collection and Management Center, and the number is CGMCC No.51328.

[0051] A method for liquid fermentation of Phellinus mycelium using fungal elicitors to increase the output of Phellinus flavonoids, characterized in that it comprises the following steps:

[0052] (1) Preparation of fungal elicitor:

[0053] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 28°C for 4 days, and use a puncher to punch out a diameter of 0.4 at the edge of the colony. Inoculate 4 pieces of bacterial blocks of 1 cm in PD liquid medium (removed agar components for P...

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Abstract

The invention discloses a phellinus igniarius mycelium liquid fermentation method by utilizing a fungal elicitor for improving the yield of flavone of phellinus igniarius, which comprises the steps of: inoculating a fungal elicitor strain for activated culture, and then inoculating and culturing a fungal block to obtain a fungal elicitor mycelium through filtration; then drying, grinding and crushing the fungal elicitor mycelium, adding the crushed fungal elicitor mycelium into ethanol for soaking, filtering the obtained solution, collecting filter residue, orderly adding a mixed solution of chloroform and normal butanol, acetone and water into the filter residue for washing, adding trifluoroacetic acid into a dry substance after the air-drying, performing boiling water bath for 0.5 to 2 hours, then filtering the obtained solution, taking a filtrate for neutralization, standing the filtrate, using a microporous filtering film with the pore diameter between 0.4 and 5mu m to filter the filtrate for sterilization to obtain the fungal elicitor, and cryopreserving the fungal elicitor at the temperature of between 10 and 20 DEG C below zero; inoculating a phellinus igniarius strain for activated culture, and then inoculating a fungal block for fermentation culture; and adding the fungal elicitor into the phellinus igniarius to continue culturing to obtain a phellinus igniarius mycelium within 1 to 5 days of liquid fermentation culture of the phellinus igniarius. The method has simple process, low cost and remarkable effect, can greatly improve the content of the flavone of phellinus igniarius cells, and is a production method with industrialization prospect.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a liquid fermentation method for increasing the yield of Phellinus flavonoids by using fungal elicitors. Background technique: [0002] Phellinus igniarius is Basidiomycotina, Hymenomycetes, Polyporales, Hymenochaetacae, Phellinus fungus, is a A precious large medicinal fungus. Traditional Chinese medicine believes that Phellinus japonica is sweet in nature and bitter in taste, and is used to treat metrorrhagia, prolapse of the anus, leukorrhea, amenorrhea, and diarrhea due to spleen deficiency. Modern pharmacological studies have shown that Phellinus has anti-tumor, immune-enhancing, anti-hepatic fibrosis and other effects. The main active ingredients of Phellinus are polysaccharides, triterpenoids and flavonoids. Among them, flavonoids have anti-oxidation, blood vessel expansion, blood lipid lowering and anti-cancer effects, and are generally extracted fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 高兴喜图力古尔姚强程显好缪静杨树德李维焕刘宇黄立红
Owner LUDONG UNIVERSITY
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