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Method for detecting malachite green through electrochemiluminescence

A malachite green and luminescence detection technology, which is applied in the field of high-sensitivity electrochemiluminescence detection, can solve the problems of high probability of false positive results and complex matrix, and achieve the effects of wide detection linear range, low detection limit and high sensitivity

Inactive Publication Date: 2011-04-06
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the combination of antigen and antibody in the ELISA method has strong selectivity, there are many types of aquatic products and complex matrices, so the probability of false positive test results is high
However, there is no relevant report on the use of electrochemiluminescence for the detection of malachite green (colorless malachite green)

Method used

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  • Method for detecting malachite green through electrochemiluminescence
  • Method for detecting malachite green through electrochemiluminescence
  • Method for detecting malachite green through electrochemiluminescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Use the MPI-B type electrochemiluminescence analyzer, configure the concentration of ruthenium terpyridine with 0.1M borate buffer solution of pH=10 to be 10 -3 mol / L solution, the high voltage of the photomultiplier tube is set to 900V, the scanning voltage is 0-1.8V, and the photomultiplier tube adopts two-stage amplification. The working electrode used is an Au electrode, and the luminescence signal of the solution is detected without adding malachite green, and the measured electrochemiluminescence intensity is 70333. Add different concentrations of malachite green respectively, so that the concentration of malachite green after mixing is 1×10 -8 mol / L, 1×10 -7 mol / L, 1×10 -6 mol / L, 5×10 -6 mol / L, 1×10 -5 mol / L etc., the measured electrochemiluminescence intensities are 75867, 80833, 84633, 86623, 90167, 92267 respectively.

Embodiment 2

[0032] Use the MPI-B type electrochemiluminescence analyzer, configure the concentration of binuclear bipyridyl ruthenium (n=6) with 0.1M borate buffer solution of pH=10 to be 0.4×10 -3mol / L solution, the high voltage of the photomultiplier tube is set to 900V, the scanning voltage is 0-1.8V, and the photomultiplier tube adopts one-stage amplification. The working electrode used is an Au electrode, and the luminescence signal of the solution is detected without adding malachite green, and the measured electrochemiluminescence intensity is 893,000. Add different concentrations of malachite green respectively, so that the concentration of malachite green after mixing is 1×10 -12 mol / L, 1×10 -11 mol / L, 1×10 -10 mol / L, 1×10 -9 mol / L, 1×10 -8 mol / L etc., the measured electrochemiluminescent intensity is 1.45×10 6 , 1.59×10 6 , 1.73×10 6 , 1.85×10 6 , 1.95×10 6 .

Embodiment 3

[0034] Use the MPI-B type electrochemiluminescence analyzer, configure the concentration of ruthenium terpyridine with 0.1M borate buffer solution of pH=10 to be 10 -3 mol / L solution, the high voltage of the photomultiplier tube is set to 900V, the scanning voltage is 0-1.8V, and the photomultiplier tube adopts two-stage amplification. The working electrode used is an Au electrode, and the luminescence signal of the solution is detected without adding colorless malachite green, and the measured electrochemiluminescence intensity is 85580. Add different concentrations of colorless malachite green respectively, so that the concentration of colorless malachite green after mixing is 1×10 -8 mol / L, 1×10 -7 mol / L, 1×10 -6 mol / L, 5×10 -6 mol / L, 1×10 -5 mol / L etc., the measured electrochemiluminescence intensities are 88700, 92820, 95000, 97375, 102050 respectively.

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Abstract

The invention belongs to the field of high sensitivity electrochemiluminescence detection, and in particular relates to a method for detecting malachite green through electrochemiluminescence. The method comprises the following steps of: dissolving a certain amount of bipyridyl ruthenium complex and malachite green in phosphoric acid or boric acid buffer solution, and scanning in a potential range of 0 to 1.8V by an electrochemical method; and detecting the malachite green by using mononuclear terpyridyl ruthenium and binuclear terpyridyl ruthenium respectively, wherein the detection range of mononuclear ruthenium is 1*10<-8> to 1*10<-5>mol / L, and the lowest detection limit is 1*10<-8>mol / L; and the detection range of binuclear ruthenium is 1*10<-12> to 1*10<-8>mol / L, and the lowest detection limit is 1*10<-12>mol / L. By the method, the malachite green can be more effectively detected, the detection limit is lower, the detection linear range is wider, sensitivity and repeatability are high, and the test can be finished instantaneously.

Description

technical field [0001] The invention belongs to the field of high-sensitivity electrochemiluminescence detection, in particular to a method for electrochemiluminescence detection of malachite green (colorless malachite green). technical background [0002] Malachite green is a toxic triphenylmethane chemical that is both a dye and a fungicide. Malachite green can cause cancer and has been banned in many countries. Malachite green is cheap and effective in treating fish diseases, especially for saprolegniasis and melon worm disease, but there is no suitable alternative drug in the field of disease prevention. Therefore, fishermen are still fighting fish infections It is used for fungi, and is also used by transporters for disinfection to prolong the survival time of fish in long-distance trafficking. In addition, users do not have enough awareness of the hazards of malachite green, so malachite green is banned more than ever. Studies have shown that the metabolite of malach...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 刘凤玉孙世国杨雪
Owner DALIAN UNIV OF TECH
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