Malachite green fast detecting method and fast detecting kit

A detection kit, malachite green technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of less malachite green, restricting research and development, complex matrix components, etc., and achieve strong applicability and the effect of inhibiting fat dissolution

Inactive Publication Date: 2007-11-07
ZHEJIANG UNIV +1
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  • Abstract
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  • Claims
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Problems solved by technology

As far as the former is concerned, most manufacturers only produce and sell solid-phase extraction columns for detecting malachite green in water bodies, and aquatic products have become a difficult point restricting research and development due to their complex matrix components and high requirements for pre-treatment processes.
At present, there are few manufacturers that conduct malachite green detection for aquatic products, and the operation steps are cumbersome

Method used

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  • Malachite green fast detecting method and fast detecting kit

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Embodiment Construction

[0027] Production of chromatography column

[0028] The diameter of the cylinder is the same as that of a 5mL pipette tip, and it can be made into the shape of a syringe. A sieve plate (sieve plate 1) is installed at the bottom to prevent the filled adsorbent from flowing down. Add 0.5 g of silica gel for column chromatography on the sieve plate 1, fill it in and tap it firmly with a suction ear ball. The sieve plate 2 is added, and the seal between the sieve plate and the cylinder is required to prevent the derivatizing agent powder on it from flowing into the adsorbent layer with the sample liquid, which will interfere with the final observation and color development. Add 0.3 g of a derivatizing agent (lead dioxide: diatomaceous earth = 1:1) to the sieve plate 2, and tap it lightly. Then, add sieve plate 3, and add 0.3g of medium-sized alumina on it, and finally add sieve plate 4 to prevent the filler in the column from being scattered and lost during transportation and pl...

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Abstract

The invention discloses a fast testing process of malachite green, which settles after mixed the measured sample and withdraw medicinal preparation, take the supernatant into the chromatography column, observes most lower level absorbent color change in the chromatography column, if forms the green clitellum, might judges that malachite green contains in the sample; the withdraw medicinal preparation is the mixture of acetonitrile and ethyl acetate solution and the acidic aluminum oxide, of which the volume ratio of the acetonitrile and ethyl acetate is 1: 15-30, the quality of the states measured sample and the mixture solution of acetonitrile and ethyl acetate volume ratio is 1:3; the quality ratio of the measured sample and the acidic aluminum oxide is 2:1. The invention used acetonitrile-ethyl acetate solution to withdraw the malachite green more completely and remarkably reduce the toxicity which is used in the reagent, also had stronger service ability to a fat product with higher content.

Description

technical field [0001] The invention relates to a rapid detection method of malachite green and a rapid detection kit. Background technique [0002] At present, the detection of malachite green mostly adopts high performance liquid chromatography, liquid chromatography tandem mass spectrometry and gas chromatography. The liquid phase method is cumbersome and takes a long time. Relatively speaking, the gas chromatography method is relatively simple, but the equipment used in both is relatively expensive, and the technical requirements for the experimenters are very high. It is not suitable for on-site detection, and the reagents used (Acetonitrile) is very toxic. [0003] There are two types of rapid detection methods currently on the market: malachite green solid-phase extraction columns, enzyme-linked immunosorbent assay kits, and test strips. As far as the former is concerned, most manufacturers only produce and sell solid-phase extraction columns for malachite green det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 沈建福张岳李蕾蕾陈秋平
Owner ZHEJIANG UNIV
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