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Immobilized biological agent and method for degrading bice green contamination of cultivation aquifer

A technology of immobilized microorganisms and preparations, applied in biological water/sewage treatment, immobilized on/in organic carriers, chemical instruments and methods, etc., can solve problems such as predation by aquatic organisms and difficulty in achieving satisfactory repair effects, etc. To achieve the effect of ease of use

Inactive Publication Date: 2008-03-05
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that when microorganisms are used to repair the polluted bottom mud of the water body, the free microorganisms that exist there are difficult to settle down and fully contact with the bottom mud, and at the same time, they are easy to be preyed on by aquatic organisms, and it is difficult to achieve a satisfactory repair effect.

Method used

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  • Immobilized biological agent and method for degrading bice green contamination of cultivation aquifer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Bacterial classification is inoculated in rich culture medium (medium composition is: in 1000ml: glucose 5g; KH 2 PO 4 1g;K 2 HPO 4 ;1g;NH 4 NO 3 1g; MgSO 4 ·7H 2 O 10mg; CaCl 2 0.5mg; yeast extract 5g; peptone 10g; adjust pH to 7.0-7.5; 121°C, 103kPa, 20min after damp heat sterilization), 35°C, 150rpm enrichment culture for 24h, centrifuge, wash with phosphate buffer, and concentrate Add the bacterial suspension into the aqueous solution containing sodium alginate, so that the final concentration of sodium alginate is 2%, and the bacterial content is 1.0 g / L. The mixture was added dropwise to 5% CaCl 2 solution, fixed at 4°C for 16h. Finally, after washing with phosphate buffer solution, the final immobilized microorganism preparation is obtained, the bacteria content is 0.188% (m / m), and the diameter of the ball is 2-3mm. The preparation is inserted into 2 mg / L malachite green aqueous solution according to 2% (m / m) inoculum amount, and after 6 days, the d...

Embodiment 2

[0025] The bacteria were inoculated in the enrichment medium (the composition of the medium was the same as in Example 1) for 24 h at 35° C. and 150 rpm for enrichment, centrifuged, washed with phosphate buffer, and the obtained concentrated bacterial suspension was added to an aqueous solution containing sodium alginate to make the final The concentration of sodium alginate is 2%, and the bacteria content is 1.5g / L. The mixture was added dropwise to 5% CaCl 2 solution, fixed at 4°C for 16h. Finally, after washing with phosphate buffer solution, the final immobilized microorganism preparation is obtained, the bacteria content is 0.282% (m / m), and the diameter of the ball is 2-3mm. The preparation is inserted into 2mg / L malachite green aqueous solution according to 2% (m / m) inoculum amount, and after 6 days, the degradation rate of malachite green is 89.2%.

Embodiment 3

[0027] The bacteria were inoculated in the enrichment medium (the composition of the medium was the same as in Example 1) for 24 h at 35° C. and 150 rpm for enrichment, centrifuged, washed with phosphate buffer, and the obtained concentrated bacterial suspension was added to an aqueous solution containing sodium alginate to make the final The concentration of sodium alginate is 2%, and the bacteria content is 2.0g / L. The mixture was added dropwise to 5% CaCl 2 solution, fixed at 4°C for 16h. Finally, after washing with phosphate buffer solution, the final immobilized microbial preparation is obtained, with a bacterial content of 0.375% (m / m), and a ball diameter of 2-3 mm. The preparation is inserted into 2 mg / L malachite green aqueous solution according to 2% (m / m) inoculum amount, and after 6 days, the degradation rate of malachite green is 88.7%. It can be seen that the preparation with 0.282% bacteria content has the best degradation effect on malachite green.

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Abstract

The present invention is one kind of immobilized microbial preparation and the method of degrading malachite green pollutant in aquiculture water therewith. The immobilized microbial preparation is prepared with Pantoea sp. M3 strain as effective component and through the following steps: 1. inoculating M3 seed to enrichment culture medium, culturing at 30-37 deg.c and 150 rpm for 18-30 hr, centrifuging, and washing with phosphate buffer to obtain concentrated bacterial suspension; 2. dissolving sodium alginate in water through heating, cooling to room temperature, mixing with the concentrated bacterial suspension; 3. dropping the mixture into 5 % concentration CaCl2 solution to form calcium alginate pellets and cross-linking to fix at 4-8 deg.c; and 4. washing the calcium alginate pellets with phosphate buffer. The present invention has high malachite green pollutant eliminating rate.

Description

technical field [0001] The invention relates to the immobilization technology of the Pantoea sp. M3 bacterial strain, and also relates to the microorganism restoration technology of malachite green polluted bottom mud. Background technique [0002] Malachite Green (Malachite Green, MG) is a synthetic triphenylmethane dye, which has been widely used as an insect repellent and fungicide to kill parasites, protozoa and molds in fish eggs outside of aquatic animals. wait. However, malachite green and its metabolite cryptomalachite green are easy to accumulate in organism tissues, are not easy to metabolize, and have high carcinogenic, teratogenic and mutagenic effects. Therefore, many countries such as the United States, Canada, Japan, and the European Union have listed it as a banned drug for aquaculture. my country also included malachite green in the "List of Veterinary Drugs and Their Compounds Prohibited for Food Animals" in May 2002. However, since malachite green is qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N1/20C02F3/34
Inventor 张彤晴潘建林吴光红林海周刚
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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