Preparation method and application of mercury ion detection probe
A mercury ion and probe technology, which is applied in the field of preparation of mercury ion probes, can solve the problems of complex operation, high cost, and long time consumption, and achieve the effect of simple operation, low cost, and low energy consumption
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Embodiment 1
[0025] The present embodiment relates to a preparation method for detecting mercury ion probes, said method comprising the steps of:
[0026] Step 1, add 10ml of purified gold nanorods with a molar concentration of 0.1nM to 5ml of a hexadecylmethylammonium bromide solution with a molar concentration of 0.01M, stir vigorously at room temperature, and then add 1ml of gold nanorods with a molar concentration of 0.01 M ascorbic acid solution, 1ml silver nitrate solution with a molar concentration of 5mM, 1ml sodium hydroxide solution with a molar concentration of 0.01M, and continue to stir vigorously for 10 minutes; centrifuge the above solution at 5000-8000rpm for 5min, and use deionized water Disperse and configure a gold-silver core-shell nano-solution with a molar concentration of 0.05nM;
[0027] Step 2: Add 1 μL of isothiocyanate-malachite green Raman molecules with a molar concentration of 0.1 mM to 1 mL of a newly prepared gold-silver core-shell nano-solution with a molar...
Embodiment 2
[0034] This embodiment relates to a preparation method for detecting mercury ion probes, said method comprising the steps of:
[0035] Step 1, add 10ml of purified gold nanorods with a molar concentration of 0.5nM to 50ml of hexadecylmethylammonium bromide solution with a molar concentration of 0.1M, stir vigorously at room temperature, and then add 1ml of gold with a molar concentration of 0.5nM M of ascorbic acid solution, 3ml of silver nitrate solution with a molar concentration of 15mM, 3ml of a sodium hydroxide solution with a molar concentration of 0.5M, and continued to stir vigorously for 5 minutes; collected by centrifugation, dispersed with deionized water, and prepared gold with a molar concentration of 0.5nM Silver core-shell nano solution;
[0036] Step 2: Add 1 μL of isothiocyanate-malachite green Raman molecules with a molar concentration of 1 mM to 1 mL of a newly prepared gold-silver core-shell nano-solution with a molar concentration of 0.5 nM, vortex and mix...
Embodiment 3
[0042] This embodiment relates to a preparation method for detecting mercury ion probes, said method comprising the steps of:
[0043]Step 1, add 10ml of purified gold nanorods with a molar concentration of 0.25nM to 25ml of hexadecylmethylammonium bromide solution with a molar concentration of 0.04M, stir vigorously at room temperature, and then add 1ml of 0.2M ascorbic acid solution, 2ml silver nitrate solution with a molar concentration of 10mM, 2ml sodium hydroxide solution with a molar concentration of 0.3M, and continue to stir vigorously for 10 minutes; collect by centrifugation, disperse with deionized water, and prepare gold with a molar concentration of 0.25nM Silver core-shell nano solution;
[0044] Step 2: Add 1 μL of isothiocyanate-malachite green Raman molecules with a molar concentration of 1 mM to 1 mL of a newly prepared gold-silver core-shell nano-solution with a molar concentration of 0.25 nM, vortex and mix for 1 min to obtain gold-silver cores Shell nano...
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Abstract
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