Preparation method and application of mercury ion probe

A mercury ion and probe technology, applied in the field of preparation of mercury ion probes, can solve the problems of complicated operation, long time-consuming and high cost, and achieve the effects of simple operation, low energy consumption and low cost

Active Publication Date: 2015-10-14
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these techniques can provide relatively sensitive detection, their cost is high, the operation is complicated, and it takes a long time; a simple, fast, selective, and highly sensitive mercury ion probe has not been reported.

Method used

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  • Preparation method and application of mercury ion probe
  • Preparation method and application of mercury ion probe
  • Preparation method and application of mercury ion probe

Examples

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Effect test

Embodiment 1

[0025] This embodiment relates to a method for preparing a probe for detecting mercury ions. The method includes the following steps:

[0026] Step 1. Add 10ml of purified gold nanorods with a molar concentration of 0.1nM to 5ml of a 0.01M cetylmethylammonium bromide solution, stir vigorously at room temperature, and add 1ml with a molar concentration of 0.01 M ascorbic acid solution, 1ml silver nitrate solution with a molar concentration of 5mM, 1ml sodium hydroxide solution with a molar concentration of 0.01M, continue to vigorously stir for 10 minutes; centrifuge the above solution at 5000-8000rpm for 5min, collect it with deionized water Disperse, configure a gold-silver core-shell nano solution with a molar concentration of 0.05 nM;

[0027] Step 2. Add 1 μL of isothiocyano-malachite green Raman molecules with a molar concentration of 0.1 mM to 1 mL of a newly prepared gold-silver core-shell nano solution with a molar concentration of 0.05 nM, vortex and mix for 1 min to obtai...

Embodiment 2

[0034] This embodiment relates to a method for preparing a probe for detecting mercury ions, and the method includes the following steps:

[0035] Step 1. Add 10ml of purified gold nanorods with a molar concentration of 0.5nM to 50ml of a 0.1M hexadecylmethylammonium bromide solution, stir vigorously at room temperature, and add 1ml with a molar concentration of 0.5. M ascorbic acid solution, 3ml silver nitrate solution with a molar concentration of 15mM, 3ml sodium hydroxide solution with a molar concentration of 0.5M, continue to vigorously stir for 5 minutes; collect by centrifugation, disperse with deionized water, and prepare gold with a molar concentration of 0.5nM Silver core shell nano solution;

[0036] Step 2. Add 1 μL of isothiocyanato-malachite green Raman molecules with a molar concentration of 1 mM to 1 mL of a newly prepared gold-silver core-shell nano solution with a molar concentration of 0.5 nM, vortex and mix for 1 min to obtain a gold-silver core Shell nanorod ...

Embodiment 3

[0042] This embodiment relates to a method for preparing a probe for detecting mercury ions, and the method includes the following steps:

[0043] Step 1. Add 10ml of purified gold nanorods with a molar concentration of 0.25nM to 25ml of a 0.04M hexadecylmethylammonium bromide solution with a molar concentration of 0.04M. Stir vigorously at room temperature, and add 1ml with a molar concentration of 0.2M ascorbic acid solution, 2ml silver nitrate solution with molar concentration of 10mM, 2ml sodium hydroxide solution with molar concentration of 0.3M, continue to vigorously stir for 10 minutes; collect by centrifugation, disperse with deionized water, and prepare gold with molar concentration of 0.25nM Silver core shell nano solution;

[0044] Step 2. Add 1 μL of isothiocyano-malachite green Raman molecules with a molar concentration of 1 mM to 1 mL of a newly prepared gold-silver core-shell nano solution with a molar concentration of 0.25 nM, vortex and mix for 1 min to obtain a g...

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Abstract

The invention discloses a preparation method and application of a mercury ion detection probe. The method comprises the following steps of: 1, adding purified gold nanorods into a cetyl methyl ammonium bromide solution, stirring, then orderly adding an ascorbic acid solution, a silver nitrate solution and a sodium hydroxide solution, stirring, and centrifuging to get a solution A; 2, adding isothiocyano-malachite green Raman molecule into the solution A, eddying and mixing uniformly to get a mixed liquid B; 3, incubating the mixed liquid B in a dark condition, until realizing adsorption equilibrium, therewith, obtaining the final product, namely the mercury ion detection probe. The invention also relates to the application of the mercury ion detection probe, the mercury ion detection probe can be used for detecting the solution containing mercury ions. The probe prepared by the method disclosed by the invention is high in sensitivity, high in accuracy, quick in detection speed, simple to operate, low in cost and low in energy consumption, and can be widely used in the fields of environment detection, food safety, diseases detection, and so on.

Description

Technical field [0001] The invention belongs to the field of chemical industry, and specifically relates to a preparation method and application of a mercury ion probe. Background technique [0002] The pollution of heavy metal ions, especially mercury ions, in the environment is becoming more and more harmful to human health. Therefore, the development of a fast and ultra-sensitive method for detecting mercury ions has become a focus of analysis and detection research. Currently, detection methods are usually used, such as the single-molecule fluorescence spectroscopy technique reported by M. Mivelle in the 2012 nano Lett magazine, and the inductively coupled plasma technique reported by S.M. Majedi in the 2012 Anal.Chem magazine. Although these technologies can provide relatively sensitive detection, they are costly, complicated to operate, and time-consuming; a simple, rapid, selective, and sensitive mercury ion probe has not been reported yet. Summary of the invention [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 陈守慧刘定斌王智华崔大祥
Owner SHANGHAI JIAOTONG UNIV
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